The golden lingzhi strain Toc1 (Tomophagus cattienensis)is a new strain that was discovered in the Cat Tien National Park in Đong Nai provine and it was identified to be a precious mushroom species. In this study, mycelial of strain Toc1 were incubated in 4 temperature levels (20 ± 1C, 25 ± 1C, 30 ± 1Cand 35 ± 1C); culture medium supplemented 5 different carbon sources (glucose, fructose, saccharose, maltose and lactose), 5 different nitrogen sources (yeast extract, peptone, ammonium clorua, ammonium nitrate and ammonium sulfate), 6 mineral salt sources (kali cloride, kali nitrate, natri nitrate, dikali phosphat, kali dihydrophosphat and magie sulfate). Beside that, mycelial of this strain were cultivated in 4 substrates added different wheat bran ratio (0%, 5%, 10% and15%). This study aims to figue out the optimal temperature, the suitable carbon, nitrogen and mineral salt source for mycelial growth of golden lingzhi mushroom strain Toc1 and also evaluating effect of the wheat bran ratio, which was supplemented on substrate, on formation and fruiting body development of this strain. The results showed that the optimal temperature for mycelial growth is 25C±1. Glucose and yeast extract were considered the most suitable carbon and nitrogen sources for mycelial growth. This strain grew very well on culture supplemented K2HPO4. The substrate containing 89% sawdust, 5% corn powder, 1% CaCO3and 5% wheat bran performed the highest BE, reached 15,97%.