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Evaluation of Apparent Nutrient Digestibility of Diets UsingFish Meal and Catfish By- Product Meal for Guinea Fowls

Nguyen Dong Hai , Nguyen Thi Kim Dong

Received: 15-03-2016 / Accepted: 17-08-2016
The experiment was carried out to evaluate the apparent nutrient digestibility of Guinea fowls at 8 and 10 weeks of age fed diets containing twodifferent protein feed sources and fourcrude protein (CP) levels. The trial used a completely randomized design with two factors and 3 replications. The first factor was two protein feed sources including fish meal and catfish by- product meal and the second factorcomposed offour dietary crude protein (CP) levels of 16, 18, 20 and 22%. The results showed that the apparent EE, NDF and ADF digestibilities of the diet with fish meal were higher than those of catfish by- product meal diet (P< 0.05). The diets contained 20% CP and 18% CP fed Guinea fowls at 8 and 10 weeks of age had the higher DM, OM, EE, CF, NDF, ADF digestibility coefficients and better N retention (P< 0,05).
State Management of Rural Clean Water at Tien Lang District, Hai Phong City

Nguyen Thi Thu Quynh, Nguyen Tat Thang, Nguyen Quoc Oanh

Received: 07-04-2017 / Accepted: 17-08-2017
Clean water is essential for the livelihood of all people, especially for the poor in the rural areas. A case study using rapid rural appraisal in Tien Lang district showed that 48.2% of people have accessed to the standard 02/BYT clean water. However, there existed several problems in State management of rural clean water at Tien Lang district with regard to effectiveness, equality and sustainability. As a consequence, the proportion of tap water that meets the standard of clean water was still low (approximate 49.7%), the rate of poor people accessing to the standard clean water was only 38.9%, and the percentage of sustainable water supply systems was only 20%. It is, therefore, needed to take clean water supply into consideration towards the goal that 70% people can access to the standard clean water by 2020.
Selection of Endophytic Microorganism from Thionic Fluvisols in Hai Phong

Nguyen Thi Minh

Received: 05-05-2017 / Accepted: 14-06-2017
This research describes the result of isolation and selection of endophytic bacteria which is considered useful for plant growth from the Thionic Fluvisols in Kien Thuy district, Hai Phong for applying in agricultural production and environmental protection. The endophytic microorganisms were selected by assessment of IAA production, inorganic phosphorus solubilizing, cellulase and amylase synthesis and pH and temperature adaptability. Of the 120 strains isolated from plant at Thionic Fluvisols region, 8 endophytic bacteria strains were selected including 2 trains belongs to Pseudomonasgenus, 3 strains of Azospirillum, 2 strains of Burkholderiaand 1 strain of Klebsiellawith strong hydrolysis enzymes and phosphorus solubilizing,high ability in plant growth production (IAA), wide pH and temperature adaption. These microorganisms will be promising solutionsfor using and reclamation ofThionic Fluvisols.
Physiological Responses of Some Groundnut Cultivars to Salinity

Nguyen Thi Thanh Hai, Bui The Khuynh, Bui Xuan Suu, Vu Dinh Chinh, Ninh Thi Phip, Dinh Thai Hoang

Received: 21-03-2013 / Accepted: 24-06-2013
The pot experiment was conducted in net house to evaluate the effects of two salinity levels (NaCl 2‰ and 4‰) on the growth and yield of 6 local groundnut cultivars. The six groundnut cultivars are Sẻ Quảng Ngãi, Lạc, Lạc Quảng Trị, Mỏ két Tây Ninh, Giấy Tây Ninh, Đỏ Thái Bình. Results showed that salinity affected significantly growth and yield of groundnut. Increase in NaCl concentration decreased main stem height, dry matter weight, CO2 assimilation rate and triggered proline production in leaves of all cultivars. Besides, increase in level of salinity resulted in decrease of yield and yield components of groundnut. Among cultivars, Mo ket Tay Ninh obtained the highest pod yield at all salinity levels. In NaCl 4‰ treatment, the highest pod yield was found at Mỏ Két Tây Ninh (3.12g/plant), followed by Lạc Quảng Trị (3.04g/plant), Giấy Tây Ninh (3.03g/plant), Đỏ Thái Bình (2.79g/plant), Lạc (2.75g/plant) and Sẻ Quảng Ngãi (2.73g/plant), respectively. Among 6 varieties studied, Mo Ket Tay Ninh and Lac Quang Tri were believed to have good growth and yield performance and can be used as parental lines for salinity tolerance breeding in groundnut.
Establishment of Plant Regeneration Protocol for Cassava (Manihot esculenta Crantz) via Somatic Embryogenesis from Immature Leaves

Do Hai Lan, Le Tran Binh, Le Van Son, Chu Hoang Ha

Received: 25-07-2014 / Accepted: 08-11-2016
The present study aimed at regenerating cassava(Manihot esculenta Crantz) via somatic embryogenesis. The explants used were immature leaves harvestedfrom 2-3-week old in vitroplantsderived from two cassava cultivars, KM94 andKM140. Somatic embryogenesis was achieved with high frequencies usingMS medium supplemented with a wide range of picloram concentrations. The results showed that the highest rate of somatic embryo formation for both cultivars was obtained when cultured in MS mediumcontaining 12 mg/l picloram. Although the number of embryos per explant was similar between two cultivars at4 weeksof culture, KM94 cultivar gave a higher rate (81,4 ± 1,7%) than KM140 cultivar (70,4 ± 2,9%). Somatic embryos were subsequently transferred to MS medium supplemented with 0.3 mg/l BAP for plantlet regeneration. All shoots with 1.0-1.5 cmin length transferredto - hormone-free MS medium formed roots in 2 weeks. Rooted plantlets were transplanted toa mixture of rice husk (40%) and sandy soil (60%) in greenhouse. This protocol required 16 to 18 weeks and was entirely appropriate for mass production of various cassava genotypes and further genetic transformation experiments.
Biological Characteristics of The Stem Borer Bactra venosana Zeller (Lepidoptera: Tortricidae) on Sedge

Nguyen Pham Hung, Nguyen Van Chi, Do Xuan Dat, Nguyen Nam Hai, The Thanh Nam, Pham Thi Vuong, Ho Thi Thu Giang

Received: 22-07-2015 / Accepted: 01-12-2015
The sedge stem borer Bactra venosanaZeller isconsidered as one of themajor pestsonsedge. The studywas carried out to examine the biological characteristics of B. venosanaunder semi-natural conditions (green house)int Nga Son, Thanh Hoa. Themean developmental time was from 55.82 ± 0.90 days, 44.57 ± 0.87 days and 33.07 ± 0.82 days at 21.83 ± 0.74oC, 25.89 ± 0.89oC and 29.83 ± 0.71oC, respectively.The number of eggs laid by a female was 69.13 eggs at 25.4oC and 91.93%RH. The egg hatching percentage was high 84,1-90,1%. Male adult longevity was significantly shorter than thefemales.
A STUDY ON FACTORS AFFECTING JOB SATISFACTION OF WORKERS AT CAM BINH SHOES COMPANY

Chu Thi Kim Loan, Dang Trang Linh

Received: 31-07-2016 / Accepted: 08-10-2016
This study focused on the factors that influence job satisfaction level of the workers working at Cam Binh Shoes Company. Theresearch usedthe primary data collected from thesurvey of 180 workers in 2015. The descriptive statistics, exploratory factor analysis (EFA) and multiple regression were employed. The study showed that about 64.4% of the surveyed workers felt satisfied with their current job. Their high evaluation wasinclinedtowardsthe training and promotion opportunities, working condition, co-worker relationship, and work nature. The average mark of bonus - welfare was the lowest. All of seven variables formulated after EFA had statistically significant effect on job satisfaction of the workers.
Isolation and Evaluation of Antibiotic Activity of Endophytic Actinobacteria on May Chang Tree (Litsea cubeba) against Pathogenic Bacteria Causing Diseases on Common Carp and Tilapia

Trinh Thi Trang, Kim Van Van, Truong Dinh Hoai, Nguyen Thanh Hai, Nguyen Van Giang, Nguyen Ngoc Tuan

Received: 02-11-2016 / Accepted: 28-12-2016
Tilapia and common carp are two main cultured species with high annual production annually in freshwater aquaculture in northern Vietnam. , hHowever, there are serious problems caused by bacterial infection. The use of antibiotics is not sufficient to mitigate outbreaks of the diseases due to antibiotic resistance of the causal agentsrate are increasing. Therefore, antimicrobial compounds are potential as a new approach to overcome the challenges of antibiotic resistance. The aim of this study is to isolate and evaluate antibiotic activity of endophytic actinobacteria from May Chang tree (Litsea cubeba) against three pathogenic bacterial species Aeromonas hydrophila GL14, Aeromonas caviaeHD60 và S. agalactiaeHY10. The results showed that 9/32 (28,2%) endophytic actinobacteria isolates could inhibit at least one target pathogenic bacteria. Three isolates MTR711, MTR622 and MTL121 showed the highest antibacterial response with minimium inhibitory concentration (MIC) ranged from 93,3 to 300,0 µl/mL, amongst these the lowest value is for MTR711 and MTR622 without significant difference. When combining three individual actinobacteria mentioned above for fractional inhibitory concentration (FIC) test, the synergistic effect was found for the pair of MTR711-MTR622 against three pathogenic bacteria chosen in this study with ∑FIC ≤0,5. The combination of two actinobacteria MTR711 and MTR622 improved bacterial inhibitory effect at least 4 times compared to individual treatment. The results are motivating enough to conduct further studies on use of endophytic actinobacteria for treating pathogenic bacteria in aquatic animals.
Effects of Replacement of Elephant Grass (Pennisetum purpureum) with Velvet Bean Hay (Mucuna pruriens) in Diets on Feed Intake, Digestibility and Nitrogen Metabolism of Growing Goats

Ngo Thi Thuy, Bui Huy Doanh, Bui Quang Tuan, Dang Thai Hai, Nguyen Thi Mai

Received: 24-09-2015 / Accepted: 09-12-2015
A feeding experiment aimed at investigating the effectof replacement of elephant grass (Pennisetum purpureum) with velvet bean hay (Mucuna pruriens) at increasing levels in the diets on feedintake, apperent digestibility, nitrogen balance and purine derivatives was conducted. A total of 8 male crossbred (Jumnapari x Saanen) goats were randomly located in a 4x4 double Latin Square design and were housed in individual metabolic cages. The goats were given ad libitum one of four experimental diets. The basal diet composed of 200g corn powder and elephant grass, elephant grass was replaced with different levels (0%, 25%, 35% and 45%) of velvet bean hay harvested at 3-4 months of age. The intake and apparent digestibility of dry matter, organic matter and NDF increased significantlyin animals fed diets replaced with velvet bean hay (P> 0,05). However, these figures were similar in animals given 35 and 45% velvet bean hay (P> 0,05). Increased levels of legume resulted in increased intake of nitrogen and nitrogen balance. The total excretion of urinary purine derivatives increased with increasing level of replacement (P> 0,05) and varied from 3.06 to 7.59 mmol/day. There was no significant difference in urinary purine derivatives between groups fed 35% and 45% velvet bean hay in diet (P> 0,05). It is suggested that elephant grass can be replaced by 35% velvet bean hay to improve nutritional values of a high fibre diet for growing goats.
Ảnh hưởng của phân bón hữu cơ và vô cơ đến sinh trưởng và chất lượng của cây hoa Thu Hải Đường và Dạ Yến Thảo trồng chậu

Vu Ngoc Thang, Dong Huy Gioi, Li Hua Tang, Nguyen Thi Ngoc

Received: 12-06-2015 / Accepted: 02-12-2015
Nghiên cứu được tiến hành nhằm đánh giá ảnh hưởng của phân bón vô cơ và hữu cơ đến sinh trưởng và chất lượng của hoa trồng trong chậu. Ba loại phân bón bao gồm phân hữu cơ (COF), vô cơ (CIF) và dung dịch dinh dưỡng tổng hợp (FSMN) đã được bố trí thí nghiệm trên 2 loại hoa trồng chậu (Thu Hải Đường - Begonia semperflorens và Dạ Yến Thảo - Petunia hybrid Vilm). Kết quả nghiên cứu cho thấy, xử lý phân bón giúp tăng sinh trưởng, quang hợp và chất lượng của hai loại hoa trồng chậu. Trong đó, phân bón COF có ảnh hưởng tốt nhất đối với các chỉ tiêu sinh trưởng và chất lượng hoa chậu so với các phân bón còn lại. Đặc biệt COF kích thích sự phân nhánh, tăng số lá, tăng hàm lượng diệp lục và hiệu suất quang hợp của 2 loài hoa trồng chậu. Giá trị cao nhất về đường kính thân, số hoa/cây, độ bền của hoa đều được quan sát ở công thức phân bón COF. Ngoài ra, Thu hải đường và Dạ yến thảo đều nở hoa sớm nhất trong công thức phân bón COF so với các công thức phân bón còn lại trong thí nghiệm.
Biochemical Characteristics of Streptococcus spp. Isolated from Tilapia with Hemorrhagic Ddiseasein Some Northern Provinces of Vietnam

Huynh Thi My Le, Pham Hong Quan, Ho Thu Thuy, Nguyen Huu Vu , Le Van Khoa

Received: 20-05-2013 / Accepted: 16-08-2013
The aim of this study was determining biochemical characteristics of Streptococcusspp isolated from tilapia with hemorrhagic diseasein some northern provinces of Vietnam. 60 samples of tilapia with hemorrhagic diseasewere collected from 4 provinces in Hanoi, Hai Duong, Hai Phong and Quang Ninh. Five bacteria species such asAeromonasspp., Pseudomonasspp., Staphylococcusspp., Flavobacteriumspp. and Streptococcusspp.were isolated. The prevalance of Streptococcusspp. was 86.67%. All Streptococcusspp isolates had typical biochemical characteristics, belong to biotype 2 and identified as S.agalactiaeby API 20 Strep system. The antibiotics tests showed that Streptococcusspp. were the strongest sensitive with enrofroxacine and doxycyline.
Assessing Genetic Diversity Tomato and Yellow Leaf Curl Virus (TYLCV) Resistance Gene ofTomato Varieties by DNAMarkers

Doan Xuan Canh, Tong Van Hai, Nguyen Hong Minh, Doan Thi Thanh Thuy

Received: 15-08-2014 / Accepted: 22-01-2015
Tomato is apopularly important vegetable in the world and Vietnam. Assessing genetic diversity and detcetion of genes for disease resistance of the existing germplasm of tomato are usefull for development ofnew varieties with high yield, good qualityand disease resistance. In this study,10 SSR markersand four markers related to TYLCV resistance genes (Ty1, Ty2and Ty3) were usedto analyse genetic diversityand identify the TYLCV genes, respectively, of26 of tomatoes accessions available at food crops research institute. The results showed, that only 5 marker showedpolymorphism.26 accessions were grouped into 5 clusters with similarity coefficients threshold of 0.7 by using NTSYSpc 2.1 software. The information found in this study is very important fornew tomato hybrid breeding programs.Only four of 26 accessions carriedTy1andthe genesTy2 and Ty3 were not detected
Study on Genetic Diversity in Taro (Colocasia esculenta) by DNA Markers

Nguyen Van Giang, Vu Ngoc Lan, Tong Van Hai

Received: 08-12-2012 / Accepted: 23-01-2013
In this study, we used DNA markers (5 RAPD markersand 5 SSR markers) to screen genetic diversity in 60 samples of taro(Colocasia esculenta)that collected. Total 67 alleleswere amplified by RAPD markers, in that has 47 polymorphic alleles. 20 alleleswere amplified by SSRmarkersand 9 alleles were polymorphic. From the electrophoresis PCR products of these two directives, 60 taro accessions were grouped into 12 clusters with similarity coefficients of 0.8 by NTSYSpc 2.1 software. The information found in this study may be used for taro conservation and breeding programs.
In VitroAnti-Bacterial Activity of Herbal Extracts against Staphylococcusspp. and Streptococcus spp. Isolated from the Metritis in Cows

Nguyen Thi Thanh Ha, Nguyen Thanh Hai, Nguyen Nam Phuong, Nguyen Van Thanh

Received: 18-04-2017 / Accepted: 26-07-2017
The use of antibioticsfor the cow metritis treatment has many advantages, however, raises concerns about antibiotic residues in the meat and dairy products as well as increasing antibiotic resistance of pathogenic bacteria. Herbs have been increasingly demonstrated their significant role in the pharmaceutical industry as a biosafety solutionandan alternative to synthetic chemical drugs. The present study aimed to examine the anti-bacterial activity of herbal extracts(Polyalthia longifolia var.Pendula, Lignum Caesalpiniae sappari, Excoecaria cochinchinensis Lour.,Clerodendron fragrans Vent, Wedelia calendulaceae Less, Cudrania tricuspidata, Pseuderanthemum (Ness)Radik, Lactuca indica L)against Staphylococcusspp. and Streptococcusspp.isolated from cow metritis. The resultsshowed that the extractionefficiency varied from 9.60% (Lignum Caesalpiniae sappari) to 14.32% (Wedelia calendulaceae Less). At the concentration of 100 mg/ml, all the extracts showed good anti-bacterial activity against Staphylococcusspp. and Streptococcusspp. For Streptococcusspp., the bacterial inhibition zone varied from 11.80 mm (Cudrania tricuspidata(Carr) to 25.00 mm (Clerodendron fragrans Vent). The diameter of the bacterial inhibition zone of Staphylococcusspp. varied from 10.28 mm (Cudrania tricuspidata(Carr)) to 25.30 mm (Wedelia calendulaceae Less). The extract solution of Clerodendron fragransVent and Wedelia calendulaceaeLess showed the highest anti-bacterial activity. This extract remained its anti-bacterial activity against Staphylococcus spp. and Streptococcusspp. at concentration of 0.20mg/ml.
Cow - calf Reproduction Systems and Effect of Concentrate Supplement at Pre- and Post-partum on Reproductive Performance of Cross-bred Brahman CowsRaised in Households in Binh Dinh Province

Nguyen Xuan Ba, Dinh Van Dung, Nguyen Thi Mui, Nguyen Huu Van, Hoang Thi Mai, TranThanh Hai, David Parsons, Rowan Smith, Jeff Corfield, Laurie Bonney

Received: 18-04-2017 / Accepted: 26-07-2017
The objective of this study was to evaluate(1) cow - calf reproduction system and (2) effects of concentrate supplement at pre - and post partrum on reproduction performance of Brahman cows. Total 66 cattle raising households (10%) in Tay Giang commune, Tay Son District, Binh Dinh province) were surveyed. In addition, 255 Cross-bredBrahman cows were evaluatedforreproductiveperformance, and 163 calves were weighted from birth to 6 months of age. On - farm experiment and demonstration of the effect of pre- and post-partum supplementary feeding on cow-calf performance were conducted from January 2014 to December 2016. The survey results showed that the cow - calf system in the communes has been moving from extensive to semi-intensiveone. Cows accounted for 50% of cattle surveyed, of which 90% were Zebu crossbred. Cow feeding systems were mostly grazing and stall feeding (97%). A long calving interval (15.9 months), lack of nutrition for cows at pre - and post - partum, and poor feeding management were identified. The experiment showed that improved supplementation for cows at pre - and post - partum could shorten calving to conception interval from 212 to 176 days (P < 0.05). The on - farm demonstration confirmed that post - partum supplementation can reduce calving interval and the techniques were well accepted by farmers in the region.
Sustainable Livelihood Development of Forest-dependent People in the Highland of BacKan Province

Nguyen Hai Nui, Nguyen Quoc Chinh, Do Quang Giam, Nguyen Thanh Lam

Received: 05-01-2018 / Accepted: 12-02-2018
The aim of this study was to assess sustainable livelihood development of forest-dependent people in the highland areas of Bac Can province. Data were collected through direct interviews of265 households living near forests in the districts of Ba Be and Na Ri. Descriptive statistics, comparisonsusing student T-test, Chi-square and Likert scale were used. Results revealed ahigh degree of the househods’ forest-dependence, limited income and medium sustainable livelihood development. High level of forest dependence was evidenced withthe fact that the higher forest-dependent strategy the lower level of sustainable development of livelihood. To enforce the policy of sustainable livelihood development, the government should adopt measures to reduce reliance on the forest, improve income for the households, and maintain social relationships and ecological environment.
Developing Agritourism in Thach That District, Hanoi City

Thai Thi Nhung, Tran Manh Hai, Doan Bich Hanh

Received: 27-03-2024 / Accepted: 02-07-2024
The study was conducted to evaluate the current status of agritourism development in Thach That district, Hanoi City. Primary data were collected from 150 tourists, 100 local farmer households, 05 tourism management officers at district and commune levels and 03 tourism experts. The research results indicate that agricultural tourism was developing fragmentarily and facing with many difficulties in land mechanisms, monotony of tourism products, lack of connection with travel agencies, lack of tourism technical facilities and human resources for agritourism. To promote the development of agritourism, Thach That district needs to have specific mechanisms and policies on land, raising awareness and tourism skills for the community, diversify and improve the quality of products, and strengthen promotion and advertising for agritourism.
Factors Affecting Application of Management Accounting at Small and Medium - Sized Enterprises in the Trade and Service Sector in Hanoi City

Tran Nguyen Thi Yen, Phan Le Trang, Phi Thi Diem Hong, Nguyen Thi Hai Binh

Received: 28-11-2023 / Accepted: 12-06-2024
The purpose of this research was to analyze the factors affecting application of management accouting at Small and Medium- Sized Enterprises in the trade and service sector in Hanoi city. The data were collected from the seveys of 153 small amd medium-sized enterprises (SMEs) and then assessed using Cronbach’s Alpha method, Exploratory Factor Analysis and regression analysis. The research result shows 7 key groups of factors that affected the application of management accouting at SMEs in Hanoi city, where in the most influential factor was the awareness of managers or directors. Other factors include enterprise size and,accounting staff qualifications, enterprise culture, application cost, information technology and market competition level.
Screening Herbs and Determining Their Mixing Ratio to Create a Product for Preventing Diarrhea andImproving Weight Gain for Pigs and Chickens

Pham Hai Son, Nguyen Van Toan, Nguyen Thi Lieu, Bui Le Kha Tu, Nguyen Thi Dung

Received: 04-07-2023 / Accepted: 23-05-2024
The purpose of this study was to investigate the mixing ratio to create aherbal product from six types of extracts: Syzygium aromaticum, Glycyrrhiza uralensis, Andrographis paniculata, Zingiber officinale, Phyllanthus urinaria, andPsidium guajavato support weight gain and prevent diarrhea in pigs and chickens. The antibacterial activity was evaluated using the MIC method, screened by the Plackett-Burman matrix, and the efficacy of the herbal product on pigs and chickens was assessed.The mixing ratio of herbs was screened by Plackett-Burman matrix. The results showed that the mixture of three extracts Syzygium aromaticum, Glycyrrhiza uralensisand Andrographis paniculatamixed in a 1:1:1 ratio exhibited good antibacterial using MIC. The MIC was obtained at 2.083 mg/ml on E. colivàS. typhimurium. This herbal product formulation yielded better weight gain and disease prevention efficiency in both pigs and chickens. Results on disease preventive efficiency showd that the survival rate in pigs and chickens after 6 weeks was 100% and 98.44%, respectively. This indicates that the herbal product had significant effect on supporting the resistance of pigs and chickens and on improving weight gain for pigs and chickens due to the biologically active ingredients of herbs combined in the product.
Influence of Dietary Peptidoglycan and Probiotics Supplementattion on Growth Performance, Feed Utilization,Survivaland Hematological Parameters in Nile Tilapia(Oreochromis niloticus)

Nguyen Thi Mai, Litpaya Nettavongsa, Le Thi Cam Van, Ho Hai Yen

Received: 16-03-2023 / Accepted: 23-05-2024
The present study was conducted to evaluate the effects of dietary co-supplementation of peptidoglycan and probiotics on growth performance, survival, feed utilization, and hematological parameters in Nile tilapia. Commercial feed specialized for tilapia were supplemented with peptidoglycan and probiotics at various concentration (g/kg of diet): 0; 3+3; 5+3; 5+5; 3+5 corresponding to experimental treatments: NT1, NT2, NT3, NT4, NT5. Nile tilapia juveniles were fed on the experimental feed for 4 weeks at a ration of 5% body weight; the feed amount was daily recorded. During the feeding period, fish were weekly weighed to monitor the fish growth. After a 4-week trial, blood samples were collected for hematological analysis; the fish number and weight were used to determine the survival rate and fish growth. Results show that the co-supplementation of probiotics and peptidoglycan induced positive effects on the fish survival, growth, and feed conversion ratio, in which fish fed 3g peptidoglycan + 3g probiotics displayed the best results.
DevelopingDirect Dry LAMP Method forField Diagnosis of African Swine Fever

Mai Thi Ngan, Souriya Viliddeth, Tran Thi Huong Giang, Dong Van Hieu, Nguyen Thi Huong Giang, Dang Huu Anh

Received: 06-11-2023 / Accepted: 07-03-2024
African swine fever (ASF) is caused by the ASF virus (ASFV) of the family Asfarviridae with a mortality rate of up to 100%. Currently, studies on the LAMP method (Loop-mediated isothermal amplification) for ASF diagnosis are based onwet LAMP, which is not feasible for field application due to the requirement of cold conditions. This study aimedto evaluate the effectiveness of dry LAMP methods to support early field diagnosis of ASF. The gold standard real-time PCR method was used to evaluate the sensitivity and specificity of the direct dry LAMP method. The results show that the two-step dry LAMP method wasmore effective in DNA amplification than the one-step dry LAMP method. Both dry LAMP methods showed good DNA amplification efficiency in both storage conditions after 2 months.The sensitivity and specificity of the two-step dry LAMP method after 2.5 months at 4C were 88.1% and 100%. Thus, the dry LAMP method is promising as an early diagnosis tool for ASF, helping to support the screening of infected individuals at the field and to improve the effectiveness of prevention and control of ASF.
DevelopingDirect Dry LAMP Method forField Diagnosis of African Swine Fever

Mai Thi Ngan, Souriya Viliddeth, Tran Thi Huong Giang, Dong Van Hieu, Nguyen Thi Huong Giang, Dang Huu Anh

Received: 06-11-2023 / Accepted: 07-03-2024
African swine fever (ASF) is caused by the ASF virus (ASFV) of the family Asfarviridae with a mortality rate of up to 100%. Currently, studies on the LAMP method (Loop-mediated isothermal amplification) for ASF diagnosis are based onwet LAMP, which is not feasible for field application due to the requirement of cold conditions. This study aimedto evaluate the effectiveness of dry LAMP methods to support early field diagnosis of ASF. The gold standard real-time PCR method was used to evaluate the sensitivity and specificity of the direct dry LAMP method. The results show that the two-step dry LAMP method wasmore effective in DNA amplification than the one-step dry LAMP method. Both dry LAMP methods showed good DNA amplification efficiency in both storage conditions after 2 months.The sensitivity and specificity of the two-step dry LAMP method after 2.5 months at 4C were 88.1% and 100%. Thus, the dry LAMP method is promising as an early diagnosis tool for ASF, helping to support the screening of infected individuals at the field and to improve the effectiveness of prevention and control of ASF.
Evaluation of Growth, Biomass Potential and Seed Yield of Introduced Sunflower Accessions

Dinh Thai Hoang, Nguyen Thi Thanh Hai

Received: 07-11-2023 / Accepted: 26-01-2024
The study aimed to evaluate the growth, potentials of biomass, and seed yields of 40 s introduced sunflower accessions. The experiment was conducted in the Spring cropping season of 2023 at Gia Lam, Hanoi in a non-replicated field experiment. The result showed that the potential accessions for biomass yield belonged to medium and late maturity groups. Biomass yield had a positive correlation with leaf area. Seed yield correlated negatively with the number of heads/plant, and positively with head diameter, total seed numbers/head, and 1000-seed weight. The accessions “CA sunflower” (87.5 tons/ha), “Red Sun” (83.1 tons/ha), and “Roshia” (77.3 tons/ha) had higher biomass yield compared to the control “Aguara” 6 (76.3 tons/ha). “Sono” accession had a high biomass yield (73.9 tons/ha) and the highest seed yield (6.54 tons/ha).
Current Situation and Solutions to Promote Lifetime Learning of Students of the Faculty of Agronomy, Vietnam National University of Agriculture

Tran Thanh Hai, Tran Thi Van Anh, Nguyen Thi Nham

Received: 09-10-2023 / Accepted: 05-01-2024
The study was conducted to evaluate the current situation and propose solutions to promote lifelong learning activities of students of the AgronomyFaculty, Vietnam National Universityof Agriculture. Survey results of students, alumni and lecturers of the faculty of agronomy showed thatthe courses in the training program and extracurricular activities are fully equipped with the necessary knowledge and skills so that students can research and self-study.It is necessary to strengthen the application of digital transformation in teaching and learning, organize a variety of exchange activities, cultural exchanges, extracurricular activities, community activities, and motivate and encourage students to participate in business exchanges, increase practice time, organize more training classes on active learning methods, self-study, self-improvement, and research for students to further develop students' life skills capacity.
Morphological, Flowering and Fruiting Characteristics of some Accessions of Angelica acutilobaSieb. et Zucc Kitagawa Grown in Sapa Lao Cai

Ninh Thi Phip, Nguyen Huy Van, Nguyen Thi Thanh Hai, Dao Xuan Quang, Bui Thi Hong Anh, Nguyen Mai Thom

Received: 11-09-2023 / Accepted: 05-01-2024
Angelica acutiloba Sieb. et Zucc Kitagawa is a valuable medicinal plant for women that is widely used in traditional medicine. This plant was introduced to Vietnam, for growing in cool climates and propagated by seed. Research on flowering and fruiting characteristics of A. acutiloba Kitagawa contributes to developing varieties suitable for Vietnamese conditions. 5 accessions of A. acutiloba Kitagawa were collected in several provinces and planted in Sapa, Lao Cai from 2018 to 2020. The experiment was designed by RCBD with 3 replications. The results showed a flowering rate of 5-10% in the first year after sowing. In the second year, 589-600 days after planting, the flowering rate was about 90-95%. A. acutiloba Kitagawa bloomed in order of branch levels. The time from flowering to seed maturity for all accessions was about 2 months. The VT-ĐQ02 accession, collected in Sapa, had the lowest rate of flowering plants in the first year after sowing, high accumulation of dry matter in stem, leaves, and root, high seeding ability, and 1,000-seed weight reaching 4.1g. Individual and actual seed yields were 64.57 g/plant and 129.14 kg/ha, respectively.

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