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Characterization of An Actinomycete Strain with Bioactivity against Vibrio parahaemolyticusCausing Disease on Shrimp

Nguyen Xuan Canh, Ho Tu Cuong, Nguyen Thi Dinh, Pham Thi Hieu

Received: 12-07-2016 / Accepted: 20-11-2016
An experiment was conducted to screen and identify the actinomycete trains that are capable of antagonizing Vibrio parahaemolyticuscausing disease on shrimp. Among of 96 isolated strains, three strains were obtained as capable of antagonizing Vibrio parahaemolyticusby agar diffusion plate method. The strain number 25.2 had strongest activity with a diameter of 15 mm clear zone of bacteria. The 25.2 strain showed white colonies but white colonies with grey borders after three and seven days of incubation, respectively. The strain did not produce soluble pigments on Gause-1 medium but grew well at temperatures between 30 - 45°C and neutral pH, and was tolerant to high salt concentration medium. The 25.2 strain was able to utilize several sources of carbon and nitrogen. Results of 16S rRNA sequence analysis showed that the strain 25.2 had a similarity of 96% compared with Streptomyces aureofaciens. Based on morphological characteristics, culture, physiological and biochemical characteristics and molecular biological analyses, the strain 25.2 was identified as Streptomyces aureofaciens.
Effect of Drying Temperature on Cochinchin GourdSeed Aril Powder Quality

Vu Thi Hang, Vu Thi Kim Oanh, Nguyen Xuan Bac, Pham Mai Huong, Nguyen Thi Hoan

Received: 07-11-2014 / Accepted: 22-07-2015
New products from Gac (Momordica cochinchinensis Spreng.), an indigenous fruit of Viet Nam have been recently found in the world wide markets as the seed aril part of the fruit contains a high content of lycopene and beta-carotene. These carotenoids are currently in special demand as they are natural antioxidants with potential to prevent cancers. The present study aimed at developing processing and conservation methods for the production of powder from Gac arils. The process development focused on the selection of drying temperatures to minimize the degradation of the carotenoid after processing. The result showed that 600C is the most suitable temperature. Gac powder dried at 600C had bright red color, typical odor, and slight loss of carotenoids. Gac powders dried at higher temperaturers (70, 80, 900C) had darker colors and considerable losses of carotenoids. The ones dried at 80 and 900C lost the typical odor but a burnt smell. In addition, the final moisture content of gac powder was increased along with the increase of drying temperature.
Developing Database and Online Seraching Sytemof Popular Rice Varieties in Viet Nam

Vu Thi Luu, Tran Thi Thu Huyen, Phan Thi Thu Hong, Nguyen Thi Thao, Nguyen Thi Thuy

Received: 17-03-2015 / Accepted: 30-07-2015
Rice is the most popular food crop in Viet Nam. However, information about rice varieties and their cultivation are mostly available in textbooks and printed documents. There has been no digitalized database of rice information and no database management system (DBMS) for rice data that users can search and retrieve the information online. This paper presents a new approach for searching and retrieving rice information from a rice database and from a system on the Internet. We have collected information from reliable data sources to build a database of popular rice varieties in some regions of Viet Nam. From that, we design a database management system that allows users to perform searching, retrieving, and making report in detail about rice varieties in a convenient and efficient way. The database can be shared to many users and the online DBMS allows one to do searching anywhere with computer and Internet connection. The success in building the database and the DBMS give users an efficient way to access to information quickly and reliably, especially for agricultural researchers, rice grower and rice consumers. Up to date, the software is the first database and the first online tool for rice information searching and management in Viet Nam.
Developing Craft Villages in BacNinhProvincein the New Rural DevelopmentContext

Le Xuan Tam, Nguyen Tat Thang

Received: 09-10-2013 / Accepted: 31-12-2013
The purpose of this study wasto discernthe statusandfactors influencing new rural development in the province of Bac Ninh, and thereby to propose policy recommendations for the development of craft villagesin theprovince.Descriptive statistical and comparative methodswereused to analyze secondary and survey data from four representativecraft villages in the study area. The resultsshowedthat productionhouseholdsaccount for the highest number among all production types in study area. Besides, manual production presenteda big constraint in selling craft products in Bac Ninh. The main influencing factors identified wereincomplete/inharmonicdevelopment policiesfor craft village, lack oftrained workers and lack of market information. Increasing trained labors, developingraw materials sourcesandsupporting advanced technology applications can be recommended as key solutions.
Preliminary Environmental Risks Assessment OF Caused by Pesticide S Residues AL POIN Tin Nam Linh Commune, Nam Dan District, Nghe An Province

Nguyen Thi Thu Ha, Tran Quoc Viet, Dinh Tien Dung, Nguyen Truong Son

Received: 08-09-2016 / Accepted: 20-12-2016
Residual point in 4 villageThe pesticide store in,Nam Linh commune, Nam Dan district, Nghe An province, which was putting in usedue to the impact of pesticide warehouses was usedfrom during the period 1968 to 1978, has undergone several major disturbancesby war and human activities. At present, the Ppesticidesleftover was mainlycurrently exists –DDT as a consequence is weak due to processof landfill after 1978. The study was conducted to assess the environmental risks of the pesticide residuesalpoint by methods recommended by Vietnam Environment Administration (MONRE) and US EPA.for residual pesticides.The results showed that the area of landunder floorarearemaining warehouse platformof the store contained highDDT(totals) withconcentrations(up to2973 mg/kgsoil), (over hundreds times higher than NTR 54: 2013/MONREstandard). From the original point DDT from the formerly landfill was spread horizontally and vertically transported but not more than 7m in depth. DDT intruded, inroadsinto surface water of ponds in the vicinity (28.175mg/l)and enteredin the near pond), inplants (0.607 mg/kg banana stembody) butthere wasno signs of DDT contamination in the groundwater. The risks of chemicalsexposure of the pesticideslandfill, heavily polluted land, and polluted surface water presentarethe immediate risk of serious impactthat,need to be controlled. Other effects caused by exposure of to polluted sediment, groundwater,and DDT- contaminatedfoodshave lower values Ddare of lower riskTbut negative effects of plant pesticides alsoshould take into considerationbe controlled.
Review: Bacteriocins - Classification, Antibacterial Activities and Applications

Ninh Thi Thao, Nong Thi Hue, Dinh Truong Son, Nguyen Xuan Canh

Received: 01-06-2022 / Accepted: 27-09-2022
Bacteriocins produced bybacteria are ribosomally synthesized peptides or proteins with antimicrobial activity that either kill or inhibit the growth of closely-related or non-related bacterial strains. Bacteriocins appear to be a weapon against microorganisms due to their specific properties of natural origin, high structural and functional diversity and heat stability. Recent studies have identified bacteriocins for application in food technology as natural bio-preservatives or in agriculture as biological control agents. Bacteriocins also present a potential approach for the treatment of pathogenic microbial infections and cancer diseases in humans and a promising alternative for replacing conventional antibiotics to treat multidrug-resistant bacteria. However, the majority of application-oriented studies were mainly conducted in laboratory conditions and focused on bacteriocins from Gram-positive bacteria. In this review, we summarized the classification and antibacterial activities of bacteriocins as well as discussed current achievements and potential applications of bacteriocins in the food preservationand inagricultural and pharmaceutical fields. Our paper provides an overview of bacteriocins, thus, orients the application of bacteriocins in Vietnam.
Isolation and Characterization ofBiofilm-FormingBacteriafor ApplicationPotential in Wastewater Treatment

Tran Thi Dao, Nguyen Thi Thanh Phuong, Nguyen Thanh Trung, Nguyen Huy Thuan, Do Thi Hanh, Nguyen Xuan Canh

Received: 23-06-2020 / Accepted: 24-02-2021
Biofilm is a complex structure that is formed by the adherence of living-organisms on their surfaces. The biofilm has been well studied and effectively applied in various-different fields, particularly in solving polluted environmental issues. This research aimed to find and identify the biofilm-forming bacteria which can be used in wastewater treatment in trade villages. Total 23 strains of formed biofilm bacterium were isolated from 15 wastewater samples collected from Khac Niem village (Bac Ninh province) where produced rice noodles by spreading on the agar LB medium. There were strains, viz.B22.5, B22.8, B22.9, B22.10, B22.11, B22.18, and B22.20, were not only composed of the highest ability to form biofilm but also resisted to four or five pathogenic bacterial strains. Additionally, 04strains (B22.5, B22.9, B22.10, and B22.18) had a high emulsifying activity of biosurfactants with an emulsification index above 60%. The complementary study of identifying the biological characteristics of representative strain B22.9 showed that this strain belongs to the Bacillus spp.,which is gram-positive and having the ability to form a biofilm at high temperatures. The optimum conditions for this strain to form biofilm are on LB medium adding glucose or fructose as a carbon source at 37C, pH 7, respectively.
Effect of Some Technological Conditions on The Extraction of Phenolic Antioxidant Compounds from Seedy Banana

Lai Thi Ngoc Ha, Tran Thi Hoai, Phan Van Hieu, Ngo Thi Huyen Trang

Received: 12-04-2017 / Accepted: 29-05-2017
Appropriate conditions for the extraction of phenolic compounds and 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity of seedy banana (Musa balbisiana)were determined using the conventional liquid-solid method. Total phenolic content and antioxidant activity were influenced by type and concentration of solvent, solid/liquid ratio, temperature, and extraction time. The optimal conditions for the antioxidant phenolic extraction were as follows: acetone concentration 60% (v/v); solid/liquid ratio1/30; at 40oC in 60 minutes. Seedy banana presented total phenolic content and DPPH scavenging activity of 15.47 ± 0.45 mg Acid gallic Equivalents per g of dry matter and 103.38 ± 3.65 mol trolox equivalents per g of dry matter, respectively. This study showed that seedy banana may be considered as a potential source of nutraceuticals applicable in food and drug technologies in the future.
Application of Membrane Filtration to Harvest Microalgae Cultivated from Piggery Wastewater

Do Khac Uan, Doan Thi Thai Yen, Nguyen Tien Thanh

Received: 24-02-2016 / Accepted: 20-11-2016
Currently, many methods such as filtration, centrifugation, flotation, and flocculation for harvesting algae for biofuel production development have been studied. In this study, application of membrane filtration to harvest microalgae was evaluated in a lab-scale system. The working volume of the system was 1 l. A hollow fiber membrane (with the surface of 0.065 m2, pore size of 0.2 µm) was submerged in the system. During the experiment, the air was supplied to prevent the membrane fouling due to the attachment of the micoralgae on the membrane surface. As a result, when the air intensity increased from 0 to 0.315 l/cm2.min, the flux increased and the transmembrane pressure decreased. When the air intensity was lower than 0.189 l/cm2.min, the flux decreased and the transmembrane pressure increased rapidly. When the air intensity was maintained higher than 0.189 l/cm2.min, the flux and the transmembrane pressure were relatively constant. Operational mode of suction pump should be maintained at 5 min on: 5 min off to keep the flux and the transmembrane pressure stable. During opertation, microalgae concentration significantly affected the flux and the transmembrane pressure. In conclusion, membrane filtration could be applied to harvest the microalgae. During operation, the air intensity should be maintained higher than 0.189 L/cm2.min to help the filtration longer. Membrane filtration could be an effective method for harvesting microalgae.
The Preliminary Investigationon Cultivation Technology of Orange Caterpillar Fungus Cordyceps militaris L.ex Fr. in VietNam

Le Van Ve, Tran Thu Ha, Nguyen Thi Bich Thuy, Ngo Xuan Nghien

Received: 18-12-2014 / Accepted: 21-04-2015
Four fruiting media(MT-1, MT-2, MT-3 and MT4) were used to cultivate Cordycepsmilitaris(orange caterpillar fungus). Results showed that MT-4 had highest biological productivity(11.63± 1.34%).Among four basal substrates including three brown rice typesA, B, C and paddy riceD were assessed. With biological productivity of 10.92± 1.96%, type B brown rice considered asthe most suitable substrateto cultivate orange caterpillar fungus. However, fifth subculturestarted to showy degenerationof fungal stock as evidenced by the change offruting-body colour, the mycelial density,the number of fruiting-body primordia andbiological productivity.
Evaluation on Capacity of Professional Colleges in the Red River Delta Region at Periods of Integration

Dong Thi Van Hong, Ngo Thi Thuan

Received: 08-04-2014 / Accepted: 13-05-2014
Despite the remarkable contribution to the training progress of skilled labour for economic sectors, the professional colleges face with challenges of educational qualities and competitiveness under the globalization and economic integration. In this study, the analysis regarding the capacity of those colleges were categorized in 3 aspects as such, organization and management, education quality, and infrastructure and teaching facilities according to the requirements of the Ministry of Education and Training. The results indicated that the State-owned colleges had higher capacity in comparison with those under management of private enterprises. Regarding colleges’ capacity evaluation, the number of colleges that satisfied the requirement referred to organization and management capacity showed the highest of percentage (86.4%), while the figures for education and training qualities and the infrastructure and teaching facilities were 80,8 percent and 79,6 percent, respectively. 93.2 percent of the colleges in the province met the requirements for the first criterion on training goals and missions, while only 61.7 percent satisfied the criterion on library quality. Among 50 evaluation criteria, some criteria reached rather low value: 20% for the teaching materials, 40% for teaching material quality, 40.3 percent for financial budgeting, 45% for education transfer program and 55% for international cooperation and accommodation. These findings can serve as practical basis to find suitable solutions for improving capacity of professional colleges in professional education.
A Computerized Vision - Based Method for Automatic Detection of Tea Shoot Tips

Nguyen Thi Thuy, Vu Hai, Nguyen Thi Huyen, Pham Thi LanAnh

Received: 22-07-2015 / Accepted: 03-09-2015
For tea producers and tea researchers, counting tender shoots in a samplingarea is usually done to evaluate productivity, recordthe growth of teaor to decide the appropriate time for harvest. However, it is a tedious and time consuming task. In this paper, we proposeda computerized vision - based method for automatically detecting and counting the number of tea shoottips in an image acquired from a tea field. First, we builta parametric model of a tea – shoottipscolor distribution in order to roughly separate Regions - of - Interest (ROIs) from a complicated background. For each ROI, we then extractedsupportive (local) features with expectations that these features will only appear around an apical bud of tea shoots thanks to two measurements: the density of edge pixels and thestatistic of gradient directions. Consequently, the extracted features were put into a mean shift cluster to locate the position of tea shoottips. The proposed method wasevaluated on a set of testing images at different sitesof tea fields and different plant ages. The results showed that the system could recognize tea shoot tips with86% accuracy. It is, therefore, possibleto designacounting-assistedtool for supportingtea producers or tea researchers.
WILLINGNESS TO PAY FOR WATER QUALITY IMPROVEMENTIN HANDICRAFT VILLAGES OF BACNINH PROVINCE, VIETNAM

Le Thi Phuong Dung, Nguyen Huu Dat

Received: 15-09-2016 / Accepted: 02-11-2016
The study was conducted to survey one thousand households(HH)from 5 out of 62 villages in Bac Ninh provinceto estimate people’s willingness to pay(WTP)for water quality improvement, to identify the factors affecting people’s willingness to pay. Respondents are the heads of household. The Turnbull techniquewas usedto estimate expected willingness to payfor water quality improvement. Results of this research show thatthe average WTP is 485.273 VNDper per HHper per year. The potential fundsobtainedwould beapproximately 6 billion VNDper year (268,251USDper year) and the real fundswould havea value of 3.45 billion VNDper year (154,475 USDper year). For Bac Ninh province, the total fundingthat would really be achieved would be 100.2 billion VND per year (4.48 million USD). Potential fundinghas a value of 155.4 billion VND per year (6.9 million USD). Several factors positively impact the WTP of HH with HH type, trade-offbetween economic growth and environmental quality, using tap water, and income of HH beingsignificant statistic variables with positive coefficients. These coefficients indicate that the heads of handicraft households who have high incomes, agree to sacrifice environmental quality to get fast economic growth, andwho use tap water aremore likely to say “yes” to the given bid. The factors of village and bidhadnegative coefficients and high statistical significance.
Molecular Characterization of Mycosphaerella berkeleyiCausing Late Leaf Spot of Groundnut in Nghe An

Ngo Thi Mai Vi, Ha Giang, Tran Thi Nhu Hoa, Nguyen Van Vien

Received: 05-09-2016 / Accepted: 30-10-2016
This study identified the ITS (Internal Transcribed Spacer) sequence of 11 groundnut late leaf spot fungal isolates collected from Vietnam, mainly from Nghe An province in 2013. The ITS sequences of the 11 isolates were identical among them and highly identical (99.7 and 100%) with two available isolates of Mycosphaerella berkeleyi onthe GenBank. Rep-PCR technique (Repetitive sequence primed PCR) with 3 sets of primers designed on the repetitive sequences of bacterial genome including the repetitive extragenic palindromic (REP) sequence, enterobacterial repetitive intergenic consensus (ERIC) and BOX element, was used to investigate the genetic diversity of 33 M. berkeleyi isolates.PCR using BOX primers (BOX-PCR) produced polymorphic bands from tested fungal isolates. Rep-PCR analysis proved that fungal populations in Nghe An were of low genetic diversity. There was no correlation between Rep-PCR defined fugal groups with the origin of the isolates (location of sampling, groundnut cultivars) and morphological characteristics of the isolates (color and size of cultures)
Development of Colorimetric Sensor Using Gold Nanoparticle and Thymine-Single Stranded DNA for Rapid and Selective Detection of Mercury Ions in Water

Dong Huy Gioi, Bui Thi Thu Huong, Phi Thi Cam Mien, Nguyen Thi Thuy Hanh, Do Duc Nam

Received: 21-12-2015 / Accepted: 11-03-2016
In this study, we have developeda colorimetric sensor for rapid and selective detection of mercury ions (Hg2 + ) in water by using the gold nanoparticle solution (AuNPs) and thymine-single stranded DNA (T-ssDNA). AuNPs is used as a sensing element based on their unique surface plasmon resonance propertiesand the T-ssDNA isself-assembled on gold nanoparticles to produce the AuNPs-T-ssDNA complex. Single-stranded DNA (T-ssDNA) could enhance the AuNPs against NaNO3-induced aggregation. However, the presence of mercury ions in the complex of AuNPs-T-ssDNA will reduce the stability of AuNPs due to the formation of Hg2 + mediated T-Hg2 + -T base pairs accompanied with the AuNPs color change from red to purple or even to dark blue. As a result, Hg2 + can be detected qualitatively or quantitatively by the naked eye or by UV-vis spectral measurement. The lowest detectable concentration of mercury ions by naked eye and by the UV-vis spectral measurement was 0.06µMand1nM, respectively.
Development of Kit for Fast Identification of Streptococcus suistype2 in Pig

Le Quoc Viet, Dinh Thi Bich Lan, Le Duc Thao, Bui Tran Anh Dao, Phung Thang Long

Received: 18-04-2017 / Accepted: 12-02-2018
To develop immunochromatographic strips for direct detection of the Streptococcus suistype 2 (S. suis2) antigen,1,5x108CFU of inactivated S. suis2 mixed well with Freund’s adjuvant was injected to Swiss albino mice to produce polyclonal antibodies. Then, the S. suis 2 polyclonal antibody (360 µg/ml) was mixed with gold colloid solution (pH 8.0) to create colloidal gold probe. Results indicated that the optimal concentration of capture antibodies in test lane was 2 mg/ml. The Kit had high sensitivity and speciﬁcity and no cross-reaction with E. coli ATCC,P. aeruginosaATCC, S. aureus ATCCor other strains ofS. suis. The detection sensitivity was found to be as high as 5x105CFU. In conclusion, the Kit is easy for the use and highlyefficient forS. Suistype 2 detection.
Optimizationof Cultural Conditions for Rhizobacterium Bacillus subtilisRB.CJ41 Against Fusariumsp.

Trinh Thi Huyen Trang, Nguyen Minh Trung, Dinh Lan Trinh, Tran Thi Phuong Hanh

Received: 03-10-2023 / Accepted: 07-03-2024
Rhizobacterium Bacillus subtilisRB.CJ41 isolated from black pepper hasantifungal activity against Fusariumsp. under in vitroand ex vitroconditions. The objective of the study was to select the appropriate culture conditions of the strain B. subtilisRB.CJ41. The single effect of environmental factors including C/N source, carbon sources, (NH4)2SO4and MgSO4sourceswas investigated on the density and Fusariumsp antifungal activity of the strain RB.CJ41. The results showed that bean residue, mannitol and MgSO4had the greatest impact on density and antifungal activity of strain RB.CJ41. Using Response Surface Methodology (RSM) - Central Composite Design (CCD), a quadratic equation describing the dependence of Fusariumsp. antifungal activity with three survey variableswas determined in the form “Antifungal activity (%) = 11.66 – 0.9452A + 1.00B + 0.2713C – 0.5625AB – 0.5625AC – 0.0625BC – 2.28A2 – 2.73B2– 1.84C2.Analytical results showed theoptimal range forhigh antifungal activity of rhizobacteriumB. subtilisRB.CJ41 was7 g/lto 8 g/lfor bean residue, 6g/lto 7g/lfor mannitol and 4 g/lto 5 g/lfor MgSO4.
Optimizationof Cultural Conditions for Rhizobacterium Bacillus subtilisRB.CJ41 Against Fusariumsp.

Trinh Thi Huyen Trang, Nguyen Minh Trung, Dinh Lan Trinh, Tran Thi Phuong Hanh

Received: 03-10-2023 / Accepted: 07-03-2024
Rhizobacterium Bacillus subtilisRB.CJ41 isolated from black pepper hasantifungal activity against Fusariumsp. under in vitroand ex vitroconditions. The objective of the study was to select the appropriate culture conditions of the strain B. subtilisRB.CJ41. The single effect of environmental factors including C/N source, carbon sources, (NH4)2SO4and MgSO4sourceswas investigated on the density and Fusariumsp antifungal activity of the strain RB.CJ41. The results showed that bean residue, mannitol and MgSO4had the greatest impact on density and antifungal activity of strain RB.CJ41. Using Response Surface Methodology (RSM) - Central Composite Design (CCD), a quadratic equation describing the dependence of Fusariumsp. antifungal activity with three survey variableswas determined in the form “Antifungal activity (%) = 11.66 – 0.9452A + 1.00B + 0.2713C – 0.5625AB – 0.5625AC – 0.0625BC – 2.28A2 – 2.73B2– 1.84C2.Analytical results showed theoptimal range forhigh antifungal activity of rhizobacteriumB. subtilisRB.CJ41 was7 g/lto 8 g/lfor bean residue, 6g/lto 7g/lfor mannitol and 4 g/lto 5 g/lfor MgSO4.
Selecting Strains and Experimental Cultivation of Hymenopellis Radicata(Rooting Shank) on Common Local Source of Substrate in Da Nang City

Tran Thi Thu Thuy, Vo Tran Khanh Huyen, Vu Thuy Duong, Pham Chau Huynh

Received: 03-10-2023 / Accepted: 25-12-2023
Rooting shank or black root mushroom, Hymenopellis radicata(Xerula radicata, Oudemansiella radicata),is a wild edible mushroom known for its high nutritional and medicinal value and, thus, significant potential for the food and pharmaceutical industries. The objective of this study was to select Hymenopellis radicatafrom five different sources and compared under controlled growing conditions in Da Nang city, Vietnam. The substrates consisted of 80.0 w% rubber tree sawdust, 10.0 w% rice bran, 9.0 w% cornmeal, and 1.0 w% CaCO3, with 30 bags per experiment, replicated three times. The mushrooms were evaluated for their growth, biological efficiency, and fruiting body quality. Data analysis identified a superior genetic source (designated as M2), which was recommended for cultivation in Da Nang city. Mycelial growth rate on the substrate was 6.26±0,24mm/day, biological efficiency of 12.06±0.38%, total protein content in the fruiting body of 31.2 w% dry weight, lipid content of <1.0 w% dry weight, and absence of E.coliand Salmonella. Notably, Hymenopellis radicataexhibited a high cadmium uptake capacity (~3.7 mg/kg dry weight), surpassing the permissible limit of 0.2 mg/kg according to QCVN 8-2:2011/BYT, while showing no affinity for lead.
Application of HighTechnology in Agricultural Production of Households in Tam Duong District, Vinh Phuc Province

Dang Nam Phuong, Tran Huong Giang, Vu Thi Thu Huong

Received: 25-10-2022 / Accepted: 27-03-2023
This study focused on the current status of agricultural production and analyzing the factors affecting the application of high technology in the agricultural production of households in Tam Duong district, Vinh Phuc province in 2019-2021 period. The probit model was used to evaluate the factors affecting the adoption of high technology by farmers. The results of the survey and assessment from 90 households engaged in agricultural production in the area show that the organization of production and planning of high-tech agricultural production areas had a direct impact on agricultural production associated high technology of households. In addition, facilities for production were also one of the decisive factors in participating in high-tech agricultural production, i.e. with modernized and concreted intra-field transportation system, the rate ofhouseholds applying high technology in production was up to 90%. The variables of demographics., arable land, and income from agriculture were also statistically significant in this study.
Effect of Postharvest Treatment Using Anti-Browning Chemicals on Quality and Shelf-Life in Cool Storage of Lao CaiPeach Fruits

Vu Thi Kim Oanh, Vu Thi Hang

Received: 19-05-2015 / Accepted: 09-10-2015
Browning isa major problem in fresh peach preservation. In this study, ascorbic acid and citric acid were used as anti-browning agents in pre-storage treatment in order to prevent the browning and prolong storage life of peach fruit harvested from Laocai Province. The research results showed that ascorbic acid and citric acid slowed down browning process and decay as well as physiological changes in the storage fruit. Different concentrations of acids were also tested and ascorbic 1,0% was proved to be the best option among the test combinations. Peach fruit which pre-storage treated with 1.0% ascorbic acid can be preserved up to 28 days.
Effects of Culture Conditons on IAA Synthetic Ability of Endophytic Bacterial Strains HY9 and TT3

Pham Hong Hien, Tran Thi Dao, Ta Ha Trang, Hoang Thi Lieu, Nguyen Van Giang

Received: 24-06-2022 / Accepted: 22-11-2022
Endophytic bacteria living in the plant tissues support plant growth by synthesizing IAA and siderophore, inorganic soluble phosphate, and increasing disease resistance of the host plants. This study aimed to evaluate the effects of carbon and nitrogen sources, pH, temperature, and time of incubation on the concentration of IAA synthesized by two endophytic bacterial strains, HY9 and TT3, isolated from Lonicera japonica. Under various suitable culture conditions, maximum IAA of HY9 and TT3 strains was produced at pH=7 (42.9 µg/ml and 49.07 µg/ml, respectively), 35C (53.49 µg/ml and 56.73 µg/ml), and 4 days of incubation (51.47 µg/ml and 53.76 µg/ml). The suitable carbon and nitrogen sources were saccharose and NH4Cl. Comparing the nucleotide sequence of 16S rRNA of TT3 strain with nucleotide sequences of 16S rRNA of other bacterial strains on GenBank revealedthat TT3 strain had a very closed relationship with Klebsiella variicola strain 1, thus,strain TT3 was named Klebsiella variicola TT3.
Enhancing the Antimicrobial Activity of Pectin-Alginate Biofilm and its Application in Preservation of Fresh Passion Fruits (Passiflora edulisSims)

Nguyen Trong Thang, Nguyen Thi Thu Nga, Hoang Thi Minh Nguyet, Nguyen Thi Bich Thuy

Received: 28-03-2022 / Accepted: 21-10-2022
The present study was aimed to enhance the antimicrobial activity of Pec-Alg film-forming solution. Nanobubbles (NBs), cinnamon essential oil (Cinna) and chlorine (Chlo) were three potential substances added to Pec-Alg solutions. The microbial inhibitory effect was evaluated in vitro, and the Pec-Alg solutions were directly applied to passion fruits to evaluate the antimicrobial effect, the rate of disease transmission during storage, and the quality changes of passion fruit through physiological, mechanical and biochemical parameters. The results showed that the solutions of Pec-Alg supplemented with NBs or Cinna or Chlo all had high inhibitory effect in vitro conditions with 86.7%, 74.1% and 93.6%, respectively. Moreover, they had a positive inhibitory effect on microorganisms on passion fruits in the following order: Pec-Alg-Chlo > Pec-Alg-NBs > Pec-Alg-Cinna, of which Pec-Alg-Chlo solution had the highest inhibitory effect, reaching 84.4% at 1 hour after coating and 43.6% after 30 days of storage.They were also significantly effective to limit the rate of disease transmission during storage, contributing to prolong the shelf life of passion fruits up to 33 days, which means an increase of 15 days compared to the control and an increase of 9 days compared to Pec-Alg coating without addition ofany antimicrobial agents.
Economic Efficiency of High-Tech Application in Production and Business at Fruit Growing Cooperatives in Mai Son District, Son La Province

Vu Thi Hai, Phi Thi Diem Hong, Tran Quang Trung, Le Thanh Ha, Bui Thi Mai Linh

Received: 12-04-2022 / Accepted: 27-09-2022
This study focused on assessing the current situation and economic efficiency of high technology application of fruit-growing cooperatives in Mai Son district, Son La province. The level of high technology application of 50 cooperatives was determined based on the actual situation of high technology adoption in five main stages of production. Research results showed that the level of high technology application at the cooperatives was low, focusing mainly on input and production stages. The study also showed that the economic efficiency of cooperatives applying high technology at different levels wasignificantly different in total revenue and cost of profit. The cooperatives applying high technology at levels 3 and 4 obtained higher economical efficiencies than those applying at levels 1 and 2. Based on the results from the research, a number of solutions in terms of capital, market, infrastructure, land, and capacity were proposed in order to improve the economic efficiency inhigh technology adoption at fruit growing cooperatives in Mai Son district, Son La province.
Factors Influencing on the Decision of using Information Technology for Accounting Work at Small and Medium-Sized Enterprises in Hanoi City

Phan Le Trang, Phi Thi Diem Hong, Tran Nguyen Thi Yen, Nguyen Thi Hai Binh

Received: 14-10-2021 / Accepted: 05-04-2022
The aim of the study was to identify the factors influencing the decision of using information technology for accounting work at small and medium-sized enterprises in Hanoi city. Applying the model of theory of planned behaviour (TPB) and technology acceptance model (TAM), the study developed the research framework for explanation of reason and scope in survey. The study used 186 small and medium-sized enterprises in Hanoi with 23 criteriawere analyzed by structural equation modelling (SEM).The research results showed 4 key groups of factors that affect the decision of using information technology for accounting work; among them, the most influential factor belonged to perceived behavioural control, followed by the groups of perceived usefulness, subjective norm and perceived ease of use.

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