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Survey on the PresenceofCanine Circovirus in Dogs in Hanoi

Nguyen Manh Tuong, Nguyen Thi Thanh Ha, Nguyen Thanh Trung

Received: 27-12-2022 / Accepted: 05-10-2023
This study was conducted to identify canine circovirus in dogs raised in Hanoi capital. A total of 180 swab samples were collected from dogs with non-clinical symptoms (healthy group) and dogs with abnormal respiratory, digestive, and urinary-reproductive manifestations (ill group) from animal clinics for detecting of circovirus by PCR assay. The results showed that 2/90 (2.2%) of dogs in healthy group were positive with canine circovirus, while 8 of 90 (8.9%) dogs in ill group also were positive with canine circovirus. In the ill dog group, dogs showing diarrhea symptom had the highest rate of detection for canine circovirus (about 16.7%), followed by the dogs showing respiratory and reproductive problems (6.7% and 3.3%, respectively). The results obtained in this study confirmed that canine circovirus was present in healthy and ill dogs in Vietnam.
Incidence of Otodectes cynotisInfestation in Domestic Dogs Examined at the Veterinary Hospital of Vietnam National University of Agriculture and Some Otoacariosis Treatments

Nguyen Van Phuong, Bui Khanh Linh, Nguyen Thi Hoang Yen, Nguyen Thi Hong Chien, Nguyen Thi Ngoc, Tran Van Nen

Received: 01-06-2021 / Accepted: 10-01-2022
The present paper reported the incidence of ear mite infestation caused by Otodectes cynotisin dogs examined at the Veterinary Hospital of Vietnam National University of Agriculture. The dog earwax was examined under microscope to determine mite infestation status. The mite-infected dogs were divided in two groups and treated experimentally with Dexoryl and Frontline Plus. Examination of ear wax samples using microscopics showed that the incidence of ear mite infestation caused by Otodectes cynotiswas 31.03%. In O. cynotisinfected dogs, the group aged 0 - 6 months infected with the highest rate, accounting for 46.97%. Foreign dogs were infected with the rate of 38.41%, higher than those of local dogs, 20.21%. According to style of living, free range dogs were infected with the highest rate, 57.89%. Dexoryl and Frontline Plus were both effective in the treatment of otodectes ear mite infestation in dogs with 96.55% and 100%, respectively.
Biochemical and Haematological Parameters of Female Dogs Infected with Pyometra

Su Thanh Long, Nguyen Thi Hoa, Vu NhuQuan, Trinh Dinh Thau

Received: 03-07-2017 / Accepted: 27-09-2017
Pyometra is a common reproductive disease in female dogs, which negatively affects the reproductive performance and results in high mortality in the case of delayed diagnosis and treatment. In this study, biochemical and the haematological parameters were assessed in healthy and diseased female dogs. The dogs consisted of two groups, group 1 (control) with 10 healthy dogs and group 2 with 10 pyometra-infected dogs. The results revealed that infected dogs showed a decrease in red blood cell count and haemoglobin and mean corpuscular volume while white blood cell count (neutrophil, lymphocyte and monocyte) increased. In addition, the liver enzyme value like GOT, GPT remained constant in both healthy and diseased dogs but the parameters related to kidney function such as urine and creatine production moderately increased..
Some Physiological Parameters in Sevoflurane-Anesthetized DogsDuring Sterilization

Hoang Thi Ngoc Huyen, Hoang Phuong Nguyen

Received: 20-12-2022 / Accepted: 29-06-2023
This study aimed to examine some physiological parameters including heart rate, respiratory rate, body temperature, blood pressure, and oxygen saturation in dogs that were anesthetized with sevoflurane for sterilization and to examine the associations between these parameters at different times of anesthesia. Thirty-four (34) dogs with body weights between 1.7 and 25kg were pre-anesthetized with zolazepam, tiletamine, medetomidine hydrochloride, and butorphanol, and then they were maintained with sevoflurane. Physiological parameters were recorded at different times during anesthesia. During surgical anesthesia, the heart rates and body temperatures of most dogs were in the physiological range. The respiratory rate, blood pressure, and oxygen saturation varied widely. At the time of the Sevoflurane connection, the heart rate was directly proportional to the breathing rate, and systolic blood pressure was directly proportional to temperature and heart rate. However, these associations were no longer present at 30 minutes and 60 minutes after the sevoflurane connection. At 60 minutes, oxygen saturation was inversely proportional to diastolic though this association was not present at the time of the Sevoflurane connection and at 30 minutes. The results show that sevoflurane maintenance of anesthesia in dogs was safe. Because physiological parameters could vary widely within each dog and during anesthesia, all dogs have to be monitored carefully throughout the surgical process to handle complications that might occur. On the other hand, Sevofulrane might have effects at different degrees on different physiological parameters and this potential characteristic should be investigated in future research.
Nutrient Requirements of Dogs and Cats: AReview

Le Duc Ngoan, Dinh Van Dung

Received: 08-10-2021 / Accepted: 21-01-2022
Dogs and cats are two types of domesticated animals that have been close to humans since ancient times. They are kept for house keeping, hunting, and entertainment (racing) purposes and are increasingly popular as pets. Viet Nam currently has more than 5 million families raising more than 7.5 million cats and dogs, equal to the number of largeruminants in the country. As pets, so their owners pay more attention to optimize nutrients to ensure good health (ideal body condition, neither obese nor rather thin) rather than maximize nutrients. The objective of this paper was to review the basic nutrient requirements of cats and dogs based on recommendations of NRC (2006), AAFCO (2014) and FEDIAF (2017) and some other studies.Dogs and cats are both carnivores, their energy requirements are higher than that of other livestock (4,000 kcal metabolizable energy/kg feed).The crudeprotein requirements of dogs are lower (18-23%) than those of cats (20-33%), but these are also higher than those of cattle and pigsor poultry, and the requirement for some nutrients is also very different from that of traditional livestock.
Evaluation of Effectivenessof Fecal Examination Techniques for Parasite Eggs and Oocystsin Dogs

Nguyen Thi Hoang Yen, Nguyen Than Thien, Dang Thi Phuong Thao, Nguyen Duyen Tung, Ha Quoc Viet

Received: 18-04-2019 / Accepted: 28-08-2019
The aim of this study was to evaluate the effectiveness of three fecal examination techniques: sugar floatation (SG: 1.27), saturated salt flotation (SG: 1.18) and formol-ether concentration, to determine the parasite eggs and oocysts in dogs. Examination of 270 dog fecal samples in some areas of Vinh Phuc, Thai Binh, Phu Tho and Thai Nguyen provinces, the result showed that the overall prevalence of gastrointestinal parasites in dogs is 75.18%. Twelve kinds of parasites were detected, consisting of one intestinal fluke (Heterophyids), six roundworms (Toxocara canis, Toxascaris leonina, Trichuris vulpis, Capillariasp., Strongyloides canisand hookworm), four tapeworms (Dipyllidium caninum, Diphyllobothrium latum, Spirometrasp., and Taeniasp.) and one coccidia (Cystoisosporasp.). Hookworm (58.81%) is the most prevalent in dogs, following by and Toxocara canis(31.11%). Sugar floatation technique showed the most effective for detection of roundworm eggs and coccidia oocysts, following by saturated salt floatation technique. Formol-Ether concentration one has more effective for detection of tapeworm egss and larva-containing roundworm eggs compared to the other techniques, but it cannot detect coccidia oocysts. In conclusions, the result of study showed that the choice of which fecal examination technique depending on the purpose of each study.
Epidemiology Characteristics of Canine Distemper Diseases in Dogs in Hanoi

Nguyen Thi Huyen, Pham Ngoc Thach, Nguyen Vu Son, Nguyen Huu Nam

Received: 14-03-2019 / Accepted: 10-06-2019
This study aimedto determine the prevalence of Canine distemper disease in Hanoi as an important information for disease prevention. An epidemiological study on 18244 dogs was conducted in some veterinary clinics in Hanoi from 2013 to 2018. The clinical diagnosiswas based on the specific clinical signs. Treatment and clinical outcome were followed after confirming by CDV Ag test kit.The study found that575 (3,15%) dogswereinfected with Canine distemper virus (CDV) in 14 popular dog breeds with the typiclcal gastrointestinal, respiratory and neurological symptoms. Mong Coc breed hadthe highest positive proportion (6.32%), followed by Becgiebreed (5.56%), Corgi breed (4.28%), Alaska (4.27%). The lowest positive percentage (1.62%) was observed in Rottweiler breed. The infection and successful treatment percentage significantly differred from one year to another (P <0.05). Among age groups, dogsaged 2-6 months old hadthe highest CDV positive rate (60.35%), followed by group of 6-12 months old (15.13%), under 2 months old (12,35%) and lowest in over 12 months old dogs(12.17%). The prevalence of CDV among seasonsof winter, spring and autumn were 39.13%, 35.82% and 11.83%, respectively. The infected CDV dogs also had statistically significant difference in age and season of the year (P <0.05).
Molecular Characterization of Canine Parvovirus Type 2Isolated from Dogs in Hanoi

Vo Van Hai, Nguyen Thi Yen, Dao Le Anh, Hoang Minh, Bui Tran Anh Dao, Bui Thi To Nga, Nguyen Thi Lan, Le Van Phan

Received: 27-08-2018 / Accepted: 10-05-2019
Canine Parvovirus type 2 (CPV-2) has caused a highly contagious and fatal disease characterized by vomiting and hemorrhagic gastroenteritis in dogs of all age.In this study, four diarrheal stool samples collected from Parvovirus-infected dogs were used for VP2 gene sequencing. The results of VP2 gene sequencing and analysis of present CPV strains showed high nucleotide (nt) and amino acid (aa) identities when compared with those of other reference worldwide strains, sharing 98.4 - 100% nt and 97.7 - 100% aa. The results of the phylogenetic tree showed that the Parvovirus type 2 in this study was genetically classified into two distinct groups of Feline Panleukopenia Virus (FPV) and CPV. Three strains VNUA-CP31, VNUA-CP32 and VNUA-CP35 belonged to the branch of FPV and close to the FPV in China. Parvovirus type 2 VNUA-CP17 belongsto the branch of CPV and is close to Parvovirus vaccines of the Pfizer and South Korea.
The Prevalence of H3N2 Canine Influenza Virus in Hanoi

Nguyen Thanh Trung, Nguyen Van Giap, Le Van Phan, Dao Cong Duan, Dam Van Phai, Pham Ngoc Thach

Received: 26-02-2016 / Accepted: 29-04-2016
A total of 150 nasal swab specimens collected from H3N2 canine influenza -suspected cases in Hanoi from May 2015 to February 2016werediagnosed by the RT-PCR method. The diagnostic results showed that6% of swab samples were positive for H3N2 canine influenza.Dogs infected with canine influenzavirus(CIV)showed typical clinical signs such as coughing, sneezing, difficulty to breath and fever. However, some cases occasionally developedsecondarycomplications, such as severe pneumonia. The rate of infected dogs was highest at 2-6 monthsold. The proportion of infected dogs with H3N2 in winter-spring was higher than in summer-autumn.
Laboratory Evaluation of Efficacy of Inactivated Vaccine Using TheCanine Distemper CDV-VNUA-768 Strain

Tran Van Nen, Nguyen Thi Lan, Nguyen Thi Hoa, Nguyen Huu Nam

Received: 03-11-2015 / Accepted: 14-01-2016
Canine distemper inactivated vaccines produced from CDV-VNUA-768 strain virus were evaluated for theirprotected capacity dogs by inoculating with CDV-HUA-04H strain virus. Six female Beagle dogs (six weeks old) were injected with the vaccine produced from CDV-VNUA-768 strain virus and inoculating with CDV-HUA-04H strain virus after 3 week post injection. Antibody response in dogs after vaccination against Canine distemper virus was examined by ELISA. Antibody contentsreached above critical level after 21days of vaccination andmaximum value during 35-42thday (titer mean of antibody was 1.54) after vaccination. Between from 42thday to 49thday after vaccination, antibody contents decreasedbut maintained above the critical level at49thday (titer meanof1.35). After 21 day post inoculation with CDV-HUA-04H strain virus, the vaccine groups produced specific antibody for canine distemper virus with titer mean above critical level (0.69). Result of this study showed that dogs were protected with 100 percent when they were injected with inactivated vaccine produced from CDV-VNUA-768.
Current Situation of Rickets in Dogs in Some Northern Provinces and Identification of Effective Treatment

Pham Thi Lan Huong, Nguyen Van Thanh, Pham Ngoc Thach

Received: 31-03-2017 / Accepted: 06-07-2017
The study aims to evaluate rickets in the population of 856 dogs in some northern provinces including Hanoi, Lang Son, Hai Duong, Hung Yen, Thai Binh, Hai Phong, and Quang Ninh and to identify effective treatment regimens. The results showed that the disease rate varied among 4 different breed groups and 4 age ranges, higher in exotic dog populations in that Béc giêbreed had the highest rate (14.91%). Dogs in the age range from 2 to 4 months had highest prevalence (17.20%) followed by the age range of from 4 to 6, under 2, and above 6 months of age (13.30%; 6.49% and 4.16%, respectively). Intrevenous injection with calcium had high treatment effect (recovery rate 100% after 10 days of treatment). In addition, nursery and nutrition cares played an important role in recovery process.
Pathological and Immunohistochemical Characterization of Testicular Tumor (Leydig Cell) in Dogs in Hanoi

Nguyen Vu Son, Nguyen Huu Nam, Nguyen Thi Lan, Bui Tran Anh Dao, Bui Thi To Nga, Nguyen Thi Huong Giang

Received: 21-07-2017 / Accepted: 02-07-2018
Leydig cell tumor is one of the most common testicular tumors in dogs. The objective of the present study was to examine the clinicopathologic and immunohistochemical features of canine Leydig cell tumor. Testicular tumors (Leydig cells) were found in the testicles of six dogs in veterinary clinics in Hanoi. Clinical symptoms included swelling, hyperpigmentation on the scrotal skin and/or hematuria, perineal hernia, and squatting to urinate. The tumors were macroscopically small, yellow to brown, soft and sharply demarcated with hemorrhage or cysts on cross-section. Microscopically, all cases show a similar histological appearance, being composed of round to polygonal cells with (or without) fine vacuoles in pale, abundant eosinophilic cytoplasm, a small and round eccentric nucleus, and infrequent mitotic figures. Neoplastic cells, in solid sheets (100%), angiomatoid (66.67%) or pseudo-adenomatous (50%) pattern, frequently contained testicular degeneration, lipid accumulation, hemorrhage and cystic structure. Immunohistochemically, tumor cells in all cases were positive for vimentin, inhibin-, melan-A and E-cadherin, but negative for neuron-specific enolase (NSE). These results help in the education and diagnosis of canine Leydig cell tumor and support future studies of tumor in animals.
Application of Ultrasound Method for Diagnosis of Canine Pyometritis in Hanoi and ItsTreatment

Su Thanh Long, Tran Le Thu Hang

Received: 20-06-2014 / Accepted: 30-08-2014
People’s living standard nowadays are being improved, hence, the number of people raising a pet increase dramatically. Together with cultural exchange and social and economic improvement, foreigners coming to Vietnam are increasing in number. They come to Vietnam for work or study and most of them brought their pet with. The risk of disease stemming from dog is related to dog number. Facing this reality,pet clinic units are increasing in number with modern equipment to accurately diagnose canine diseases. Ultrasound method was applied for diagnosis, especially, in case of dog pyometritis. By this method, the detecting rate of the disease was higher than clinical examination (P<0.05). The pyometritis rate in local dog breeds was lower than exotic breeds (P<0.05). The older dogs and non pregnancy dogs exhibited higher risk of carrying pyometritis than normal dogs. The recovering rate was 88.88% by surgical method and was higher than conservation treatment (65.21%; P<0.05).
Pathological Characteristics of the DogsCaused by CareVirus (CDV-768)

Nguyen Thi Lan, Bounheuang SIHOUNGVANH, Nguyen Thi Yen, Nguyen Huu Nam

Received: 20-10-2014 / Accepted: 29-11-2014
Canine distemper (CD)is an acute infectious disease caused by virusin dogs. Results of infecting virus strain Care (CDV-768) for three Berger doghybrids at2 months of age adose 106TCID50/25µlthrough the eyes, mouth,and nebulizationshowed thatinfected dogs showedsymptoms of moodiness, fatigue, appetite loss, fever, vomiting, diarrhea, and bloody diarrhea with sketchy notes on the skin, thickening/keratinizing of the soles of the feet. The macroscopic pathological lesions were found mainly in the lungs (pulmonary sectional epidemic, lung incarnate), intestinal hyperemia phenomena, hemorrhage, brain hemorrhage, swollen lymph nodes, liver degeneration, and swollen gallbladder. The microscopicpathological signs includedred blood cells inside alveoli, alveolar walls rupture, parenchymal cell degeneration, torn crushed villi,and inflammatory cell infiltration in the brain. Virus concentrated mainly in the lungs, lymph nodesandintestines. The experimental results showedthat virus strain Care (CDV-768)was highly virulentand potentially pathogenic for dogs.
Pathological Characteristics inPhu Quoc Dog Infected with Canine Distemper and Application of Immunofluorescencefor Diagnosis

Nguyen Thi Lan, Khao KEONAM

Received: 12-05-2012 / Accepted: 29-10-2012
The clinical symptoms and pathological characteristics of Phu Quoc dogs infected with Canine Distemper Virus (CDV) were studied. The first clinical signs of infected dogs were high fever, loss of appetite or not eating, vomiting or coughing, sketchy notes in abdominal skin, diarrhea and neurologic signs - walking in a circle. The gross changes were mainly found in lung and intestine including congestion and hemorrhage in lung and intestine, congestion in brain. The other gross changes were enlarged spleen, lymph nodes, swollen gall bladder, and degenerated liver. The microscopic findings were mainly in lung, intestine and brain. There were lots of red blood cells in alveoli, inflammatory brain cells, alveoli and damaged villi of intestine. In the present research, the distribution of CDV in different organs of dogs was identified by using immunofluorescent test.
A Survey on Estrogen and Progesterone Levels in Pyometra-infected Female Dogs at Ninh Kieu District, Can Tho City

Dang Thanh Son, Truong Phuc Vinh, Diep Truong Khang

Received: 18-04-2017 / Accepted: 26-08-2017
The research was conducted on 42 female dogs/bitches infected with pyometra. The results showed that the symptom appearing as abdominal enlargementwas (66.6%), followed by vaginal discharge (57.1%), fever (47.6%), poor feed ingestion (42.8%), polyuria (38.1%), and polydipsia (28.6%). The estrogen and progesterone levels in the diseased bitches were 12.47 ±8.95 pg/ml and 1.98 ±1.18 ng/ml,respectively. Thebitches in the anestrus had estrogen concentrations at 7.96 ±2.75 pg/ml and progesterone at 0.90 ±0.29 ng/mlwhereas thebitches at 8 - 10 week after parturition had estrogen and progesterone levels in average of 7.58 ±2.48 pg/ml and 1.10 ±0.54 ng/ml, respectively. Imported bitches often had pyometra higher infection (54.8%) than domestic bitches (45.2%). Bitches that have experienced a contraceptive injection (61.9%) had a higher infectionrate than those without contraception (38.1%). The rate of pyometra increased with age and bitches which had never given parturition had the highest infection rate,accountingfor 59.5%.
Genomic Sequencing and Analysis of Parvovirus Isolated from Dogsin some Northern Provinces of Vietnam

Vo Van Hai, Trinh Thi Bich Ngoc, Pham Quang Hung, Nguyen Van Tam, Vu Xuan Dang, Bui Tran Anh Dao, Le Van Phan

Received: 14-09-2021 / Accepted: 05-04-2022
Canine Parvovirus type 2 (CPV-2) is a virus that causes acute diarrheal enteritis in dogs. CPV-2 is a DNA virus and based on the VP2 gene sequence, CPV-2 is clasified into CPV-2a, CPV-2b and CPV-2c. Currently, CPV-2c is the most popular variant circulating in the world as well as in Vietnam. In this study, the genome sequences of eight canine Parvovirus strains isolated in some northern provinces of Vietnam during 2018-2020 were sequenced. The results of genome sequencing of the Parvovirus strains have obtained genetic sequences of 4269 nucleotides (nt) in length, in which the gene sequence coding for the open reading frame (ORF) corresponding to the non-structural proteins was 2007 nt and structural proteins was 2256 nt in length. The phylogenetic analysis based on the full genome sequences showed that all eight present canine Parvovirus strains belonged to CPV-2c and shared the same evolutionary brand with clade Asia-4. The whole genomic sequence data of canine Parvovirus obtained in this study will be an important source for any spatial and temporal genetic evolution studies of canine Parvovirus strains circulating in Vietnam.
Toxocariasis in Cats in Viet Nam Caused by Toxocara malaysiensis, A Novel Species, Identified by Sequence and Phylogenetic Analyses

Received: 11-10-2015 / Accepted: 28-01-2016
Toxocariasis in cats and dogs is a disease caused by a species of the genus Toxocara, family Toxocaridae, class Nematoda, including Toxocara canis (T. canis), Toxocara cati (T. cati) and Toxocara malaysiensis (T. malaysiensis), which is potentially transmissible to humans. Cats might get infested with a different ascaridoid species, a novel species, namely T. malaysiensis, which was first time found in Malaysia and China, but there is no information about this novel species in other countries. In this study, we analyzed mitochondrial atp6 and nuclear ITS2 sequences to clarify taxonomic identification for 15 strains of Toxocara sp. collected from cats from Thuong Tin and Thanh Oai districts (Ha Noi, Viet Nam). Comparative analysis of atp6 nucleotide sequences showed that all 15 Vietnamese strains of Toxocara sp. from cats were identical with Toxocara malaysiensis species from 99.3 to 100%, but with T. Cati 89.6-90.1% , with T. canis 86.7-87.9%, and with T. vitulorum 80.5-88.4%. Genetic distances between Toxocara strains of Viet Nam and T. malaysiensis showed no difference, but it was highly distant from T. cati. Phylogenetic analysis based on genetic markers of atp6 and ITS2 confirmed that all 15 strains of Toxocara sp. from Ha Noi, Viet Nam on cats were placed within the T. malaysiensis group, while the strains of T. cati, T. canis, T. vitulorum and Toxascaris leonina (an outgroup) were separated completely, clustered within their respective groups. This study has provided some additional features on genetics and classification of T. malaysiensis and also was reported the first time regarding this novel species originated in Viet Nam for epidemiological surveillance in the community.
Neospora caninum INFECTION IN CATTLE - ECONOMIC LOSS, PREVENTION AND CONTROL

Nguyen Hoai Nam, Suneerat Aiumlamai, Aran Chanlun, Kwankate Kanistanon

Received: 02-03-2012 / Accepted: 26-05-2012
Nespora caninumis a parasite which was first detected in Norwegian dogs and has been known as an important abortive cause of cattle. A high abortion rateup to 44% occurs in N. caninumpositive pregnant cows.. Besides, consequence of infection could be culling of the aborted dams, reductionof milk production and weight gain, and increase of veterinary, diagnosisand replacement purchase costs. Various methods have been studied to prevent and control N. caninuminfection in cattle. However, there are no highly effectiveapproaches available in terms ofboth epidemiological and economic aspects so far.
Molecular Biological Characteristics of Canine Distemper Virus Strain Isolated from Some Provinces in TheNorth of Vietnam

Tran Van Nen, Nguyen Thi Lan, Nguyen Van Thanh, Luong Quoc Hung

Received: 06-02-2016 / Accepted: 13-03-2016
The study was conducted to investigate molecular biological characteristics of isolated canine distemper virus (CDV) strains from some provinces in the North of Vietnam. Five virus strains in this study were isolated from clinical samples of dogs infected with CDV in Nam Dinh, Thai Binh, Hung Yen, Hanoi and BacGiang provinces in 2015. After the viral ARNs were extracted from tissue samples, RT-PCR and PCR-sequence assay were performed using specific primer pairs to amplify haemagglutinin (H) and phosphoprotein (P) genes of CDV. The products of PCR-sequence were directly sequenced using Sanger sequencing method. The sequence of H and P genes was 1824bp and 402bp in length, respectively. The high similarity of nucleotides in H and P genes between 5 virus strains was 90.05 - 99.61% and 94.81 - 99.75%, respectively. The high similarity of amino acids encoded by H and P genes between 5 virus strains was high with 89.38 - 99.5% and 92.47 - 100.0%, respectively. The phylogenetic trees showed that five isolated virus strains belonged to three different genotypes: Asia 1, Asia 2 and Classic. These results indicated that pathogenic genotypes of virus strains were circulating in northernprovinces of Vietnam. These results are useful information for producing vaccine from pathogenic virus strains to control CDV in Vietnam.
Single Nucleotide Polymorphisms (SNPs) inthe Gene Segment Coding for Cathepsin DAspartic Proteaseof Zoonotic Hookworm Ancylostoma ceylanicum

Duong Duc Hieu, Bui Khanh Linh, Nguyen Thu Huong, Le Duc Vinh, Nguyen Thi Hoang Yen, Nguyen Thi Hong Chien, Nguyen Van Phuong

Received: 02-04-2019 / Accepted: 01-06-2019
This study was conductedto determine the presence of the canine zoonotic hookworm species, Ancylostoma ceylanicum, in humans in Vietnam and to analyze the single nucleotide polymorphism in thegenesegment encoding A. ceylanicumcathepsin D aspartic protease, one of the hydrolytic enzymes with an important role in thelife cycle of hookworm. The existence of Ancylostoma ceylanicumin dogs and humans in Vietnam was confirmedby KOD-PCR. Comparative analysis of tthesequences encoding A. ceylanicumcathepsin D aspartic proteaserevealed, some mutations in exon 7, 8,and 9. Three SNPs non-synonymous mutations (one from exon 7 and two from exon 9)caused the changes in amino acid sequences of aspatyl protease.Our results can be considered forfuther studies on the development of effective vaccine against hookworm diseaseusing recombinant cathepsin D aspartic protease as an antigen.

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