Received: 16-02-2024 / Accepted: 12-04-2024
Dendrobium officinaleKimura etMigoof the genus Dendrobiumis a rare and endangered orchid species. In vitromicropropagation was carried out for preserving and developing this precious orchid genetic resource. All experiments were arranged in a randomized complete design (RCD) with three replicates. The results showed that the fruit as initial material source produced 100% survival of explants, generatingprotocorn and shoots and superior to the initial material source of using buds (rate of survived explants was only 2-3%).The addition of coconut water to the culture medium did not have a clear effect on the morphogenensis of Dendrobium officinale.The MS medium was the most suitable forrapid multiplication of orchid shoot clusters (2.56protocorn/8weeks). Adding growth regulator -NAAat a concentration of 1ppm or BAP at a concentration of 3ppm to the basal medium produced the largest number of shoots (45 protocorn/8weeks) and high shoot multiplication coefficient (2.61protocorn/8weeks). For in vitrogrowth of orchid shoots, RE mineralculture medium supplemented with 0.1% activated charcoal was optimal for plantlet regeneration.