ISOLATION, IDENTIFICATION, AND BIOLOGICAL CHARACTERIZATION OF CHITINASE-PRODUCING ACTINOMYCES FROM YOK DON NATIONAL PARK

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Ngày duyệt đăng: 25-12-2023

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Số: Tập 21 Số 12 (2023)

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Tran, D., Huynh, T., & Nguyen, A. (2024). ISOLATION, IDENTIFICATION, AND BIOLOGICAL CHARACTERIZATION OF CHITINASE-PRODUCING ACTINOMYCES FROM YOK DON NATIONAL PARK. Tạp Chí Khoa học Nông nghiệp Việt Nam, 21(12), 1517–1526. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1223

ISOLATION, IDENTIFICATION, AND BIOLOGICAL CHARACTERIZATION OF CHITINASE-PRODUCING ACTINOMYCES FROM YOK DON NATIONAL PARK

Dinh Minh Tran (*) 1 , To Uyen Huynh 1 , Anh Dzung Nguyen 1

  • 1 Institute of Biotechnology and Environment, Tay Nguyen University

    • Từ khóa

    Luteimicrobium album, chitinase activity, biofilm formation, plant-growth promotion, biocontrol

    Tóm tắt


    Developing new biofertilizers has been an area of great interest in modern agriculture. This study focused on actinobacteria possessing high chitinase activity, biofilm formation, plant growth promotion, and antifungal activity. Two chitinolytic actinomyces strains, YSS-3.3 and YWS-5.1, were isolated from soil and chitin flake samples collected from Yok Don National Park in the Central Highlands, Vietnam. These strains exhibited high specific activities of chitinases and formed biofilms. 16S rRNA gene analysis revealed that both strains had the closest evolutionary relationship to Luteimicrobium albumwith 99.41% sequence similarity and 100% query cover. Plate assays showed that the actinomyces had cellulolytic and amylolytic activities; insoluble zinc, potassium, and phosphate solubilization; and produced siderophores. UV-HPLC analysis revealed that the bacteria secreted phytohormones (IAA, GA3, and zeatin) into their cultures. Dual culture assay indicated that the bacteria possessed antifungal activity against Fusarium oxysporum. These analyses indicated that the isolated actinomyces are good candidates for further studies concerning crop production. These strainsare also potent resources for further research on their genome sequences and systems of chitinases, cellulases, and amylases.

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