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The Responses of Maize Inbred Lines to Flooding Condition at Seedling Stage

Nguyen Van Loc, Nguyen Viet Long, Nguyen The Hung, Nguyen Van Cuong, Pham Quang Tuan

Received: 02-10-2013 / Accepted: 20-11-2013
The effects of flooding on growth and biomass accumulation of maize at the seedling stage was studiedin pot experiment in greenhouse conditions. Seedlings of eleven maize inbred lines were subjected to flood and control treatment for a period of one week. Growth in terms of root system, leaf area, leaf number, leaf chlorophyll content (SPAD), plant height, and dry matter of the plant were significantly reduced in flood stressed conditions. There was clear genetic variation inresponse to flooding stress among themaize inbred lines. Three inbred lines: CLT-T10, CLT- T83 and TB23 showed better tolerance to flooding condions. These inbred lines were recommended to use in further study at molecular level of response to flooding or to use in gerplasm improvement for flooding tolerance. The current study pointed out that there is a relationship between root development and flooding tolerance in maize. Root traits might be important to consider in maize improvement for flooding tolerance.
Impacts of Urbanization on Land use Change in Dong Hoi City, Quang Binh Province

Nguyen Duc Cuong, Nguyen Quang Hoc, Nguyen Dinh Bong

Received: 08-08-2022 / Accepted: 27-09-2022
The research was conducted to determine the impact of urbanization on land use change in Dong Hoi city. Secondary data were collected from documents related to the natural and socio-economic conditions and current situations of land useduring2005-2020 period from the provincial agencies (Bureau of statistics and Department of Resources and Environment), and the Office of Resources and Environment of Dong Hoi City. Primary data were gathered by interviewing 15 city staff understanding the process of urban development and land use of the city. In addition, field survey was conducted to collate with the desk research data. The research results show that the degree of urbanization in Dong Hoi increased steadily in the period 2005-2020. Land use change in Dong Hoi was affected by 4 groups of factors: (i) The degree of urbanization, (ii) The distance to the coast, (iii) The distance to the main traffic and (iv) The population density.
Effects of Bacteriophages on the Density and Morphology of Vibriospp.

Le Hoang Bao Ngoc, Pham Hoai An, Phan Tran Hoc Khang, Tieu Ngoc Thanh, Tran Huu Hau, Phan Kim Ngan, Tran Ngoc Thao Vy, Do Thi My Anh, Vo Ngoc Tran Anh, Nguyen Thi Kim Thoa, Truong Thi Bich Van

Received: 29-11-2021 / Accepted: 27-05-2022
The study was condcuted to evaluate the effects of phages on the phenotype and density of Vibriospp. The Vibriospp. strains were isolated from An Thanh 2 and An Thanh Dong commune, Cu Lao Dung district, Soc Trang province, and 18 bacteriophage strains were obtained from the Molecular Biology Laboratory, Biotechnology Research and Development Institute, Can Tho University. The spread counting method was employed to observe the number and changes in colony morphology of Vibriospp. after treatment with bacteriophages. The results showed that there were changes in the number and phenotype of colonies of the tested bacteria with different phage strains. Specifically, seven bacteriophage strains 2, 3, 4, 5, 7, 8, and 22 significantly reduced the number of colonies of Vibriosp. strain B1.1 while four bacteriophages 1, 19, 21, and 23 increased the number of bacterial colonies. In addition, remarkable morphological changes were observed in color, size and cover of Vibriosp. strain B1.1 after treating with phage 21, 22, and 23. The findings indicated that bacteriophages exerted the ability to change the number and phenotype of Vibriospp. colonies.
Assessment of Combination and Biological Characteristics of Hybrid Combinations from some Oyster Mushroom Genetic Resources

Tran Dong Anh, Nguyen Thi Bich Thuy, Ngo Xuan Nghien, Nguyen Thi Luyen, Vu Thi Nhu Hoa, Le The Cuong, Do Thi Thu Quynh, Dinh Van Nam

Received: 22-04-2019 / Accepted: 05-04-2021
In oyster mushroom cultivation, it is very necessary to create new mushroom strains with more superior characteristics than cultivated strains.The three oyster mushroom strains PN1, PN14 and PN10 were used to generate hybrid combinations by single spore hybridization method and evaluated their hybrid combinations on the spawn culture and substrate cultivation. Thirty-eight single spore lines were isolated, including 18 single spore lines of PN1 strain, 16 single spore lines of PN10 strain and 4 single spore lines of PN14 strain. Seventy hybrid combinations have been implemented, of which three successful hybrid combinations were HP1-27 x HP14-4 (symbolized as PN1141), HP1-27 x HP14-7 (symbolized as PN1142), HP1-27 x HP14-10 (symbolized as PN1143) based on the appearance of clamp connection on the hyphae. On PDA medium, the hybrid strain PN1143 showed a lower growth rate than PN1 strain and higher than the remaining strains. On the spawn culture of boiled paddy, two strains of PN1 and PN1143 have the highest number of days to full colonization of substrates and growth rate of the hypha; the PN10 strain is lowest. On the cultivating substrate, 2 strains PN1 and PN1143 achieved higher results than the other strains in the growth rate of the hypha, fruit body size, biological efficiency were 55.46 and 54.54% respectively. The three strains of hybrid oysters are grayish-white, with stripes appearing on the mushroom caps.
Maize Root Development under Hypoxia and Waterlogging Conditions

Nguyen Van Loc, Pham Quang Tuan, Nguyen Viet Long

Received: 27-12-2018 / Accepted: 21-03-2019
Waterlogging is a major environmental stress limiting maize yield in the monsoonal areas of Asia, as well as in other parts of the world. The objective of this study was to evaluate thephenotypic variation in root development under hypoxia condition at the seedling stageofdiverse maize inbred lines in 0.1% agar-hydroponic culture. The root length (RLD), root surface area (RSAD) and root volume (RVD) under hypoxia wereinvestigated by means of WinRHIZO analysis before and after the treatment. Based on the evaluation results of 30 lines under hypoxia, root development of15 selected lines was also evaluated in soil culture. Significant phenotypic variation in hypoxia tolerance in roots was observed among studiedmaize lines. Root developmentin hydroponic and soil culture wassignificantly correlated. The results suggested that nine potential lines (VNT2, VNT3, VNT7, VNT9, VNT12, VNT13, VNT14, VNT24 và VNT25)hada high ability to develop roots under hypoxia. These resultsprovide insights into the mechanisms of waterlogging adaptation and the hypoxia tolerant materials can be usedto improve the waterlogging tolerance of modernmaizecultivars at the seedling stage.
Evaluation of Some Agrobiological Characteristics and Drought Tolerance of Imported Rice Lines in Thua Thien Hue province

Tran Minh Quang, Tran Dang Hoa, Truong Thi Hong Hai, Dinh Ho Anh, Tran Thi Phuong Nhung

Received: 05-11-2018 / Accepted: 25-12-2018
This study assesseddrought tolerance and some agro-biological characteristics of rice lines imported from International Rice Research Institute (IRRI) in Thua Thien Hue province with experiments conducted in laboratory induced drought condition and on rice field with non-active irrigation. The results of laboratory induced drought experiment showedthat introduced rice lines from IRRI hadthe germination rate in the range of 39,57-89,67% when KClO3 2% solution was used, the radicle length variedfrom 0,64cm to 3,07cm. Some of varieties showedsignificant drought tolerance when there was no watering during seedling stage and branching stage. The results of experiments on field with no irrigation showedthat the introduced rice lines from IRRI hadgrowth duration varying from 111 to127 days and belonged to medium and long-day plant group; plant heights varyiedfrom 73,5 to 122,3cm; vegetative tiller was in the range of 9,2-21,2 tillers; effective tiller was 6-17tilers. The productivity factors and theoretical productivity were relatively high in some rice varieties. Most of the rice varieties showedgood adaptation to drought in the condition of non-active irrigation in Winter-Spring crop in Thua Thien Hue province.
Assessment of Semen Quality and Artificial Insemination for Dong Tao Chicken

Le Thi Tham, Do Van Thu, Tran Xuan Khoi, Le Thi Hue, Ngo Xuan Thai, Doan Viet Binh

Received: 28-03-2017 / Accepted: 10-07-2017
The research was conducted tocollect semen of Dong Tao cocks, evaluatesemen quality, preserveand artificiallyinseminateDong Tao hens. A total of 62 cocksand420 hens with typical characteristics of Dong Tao breedrearing at households in Dong Tao, Khoai Chau, Hung Yen were selected for the study. The results showedthat the cocks had relatively slow ejaculation reflex. The semen volume, sperm activity and concentration were 0.46 ml, 76.04% and 3.26 billion/ml, respectively. The semen quality in summer was relatively low. The best semen collection interval was once a day. It was possible to use both buffer 1 and 2 to dilute semen, but buffer 1 beingmore appropriate, because after 9 hours of conservation at5oC, the sperm activity remained over50%. With the interval of 3 days between two artificial inseminationsata dose of 150 millionsperms per insemination, the rate of fertilized eggs reached over 84%. Insemination in the evening wasbetter than in the morning. The results suggestedthe applicabilityof artificial insemination for Dong Tao chickens.
Selection of Thinner Pericarp Thickness for Quality of Fresh Waxy Corn

Tran Thi Thanh Ha, Nguyen Thi Hong Ngat, Nguyen Van Ha, Duong Thi Loan, Vu Thi Bich Hanh, Vu Van Liet

Received: 19-02-2013 / Accepted: 22-04-2013
Waxy corn is commonly eaten fresh in Asian countries including Vietnam. Selection for thinner pericarp is a priority for enhancing tenderness in fresh waxy corn breeding. We have evaluated 48 of local maize cultivars and inbred lines in order to identify genetic materials that have thinner perricap for the waxy maize breeding with improved quality. The 48 cultivars and inbred lines were evaluated in the field experiment with two replication in 20012 Autumn-Winter season. Kernel pericarp thickness of cultivars and inbed lines (measured by micrometer) ranged between 51,6 to 118,9 µm. Six potential lines and cultivars with desirable pericarp thinness are D27, D34, D36, D14, D22 and D35 (D27 with 51.6 µm). Using SSR markers 28 cultivars and inbred lines were detected to have QTL controlling thinner pericarp traits. Based on phenotypic evaluatione and genetic markers, six cultivars and inbred lines, D14, D22, D27, D47, D36 and D44, possessing desirable agronomic characteristics, thinner pericarp were selected for high quality waxy maize breeding programme in Vietnam.
Growth Performance, Carcass Characteristics and Meat Quality of VCN03 Boars

Trinh Hong Son, Nguyen Que Coi, Dinh Van Chinh

Received: 24-09-2013 / Accepted: 25-11-2013
This study was carried out in Tam Diep pig research stationofThuy Phuong National Pig Research from 2009 to 2013 to evaluate growth performance, carcass characteristics and meat quality of VCN03 boars between two generations (initialgeneration and first selection (G1)). Growth performance was measured on 205 boars (G0: 114, G1: 91). Carcass characteristics were conducted on 40 boars (G0: 20, G1: 20), meat quality was determined on 36 longissimus dorsimuscle samples (G0: 17, G1: 19). Average daily gain and lean meat percentage of selected boars were higher than those of G0(P<0.05). Average daily gain, backfat thickness, longissimus dorsimuscle thickness and lean meat percentage of G0and G1were 769.51 and 829.60g/day, 10.27 and 9.38mm, 46.84 and 48.80mm, 59.74 and 61.14% respectively. Killing out percentage and carcass yield werenot significantly different between two generations (P>0.05). Killing out percentage and carcass yield of G0and G1were 80.68 and 81.26%, 72.01 and 72.30% respectively. The pH of logissimus dorsimuscle at 45 minutes (6.01 and 6.28 for G0and G1respectively), pH (5.57 and 5.45) and L* (54.39 and 53.76) were in the range corresponding to the normal meat. Average daily gain and lean meat percentage of VCN03 boars after selection were improved without affecting carcass characteristics and meat quality.
EFFECTS OF YEAST STRAINS, pH AND FERMENTATION TEMPERATURE ON WINE MADE FROM RhodomyrtustomentosaFRUIT (MANG DEN, KONTUM PROVINCE)

NguyenMinh Thuy, Nguyen Phu Cuong, Nguyen Thi My Tuyen, Dinh Cong Dinh

Received: 24-10-2013 / Accepted: 24-02-2014
The effects of yeast strains, fermentation temperature and pH on quality of Rhodomyrtus tomentosa wine were examined. At ambient temperature (28±2oC), the fermentation was induced by inoculation with Saccharomyces cerevisiae strains isolated, purified and screened from sugar palm (Borassus flabellifer) and pineapple juice in comparison with commercial yeast (initial populations of yeast raging from 104107 cells/ml). The medium was adjusted before fermentation to five different pH values (3.44.2). The effect of fermentation temperature (20 and 28±2°C) on strain population was also studied. The resulting wines were chemically analyzed. Pure cultures of Saccharomyces cerevisiae isolated from sugar palm significantly yielded in ethanol production higher than other strains in the fermentation at 28±2°C.. Yeast strains performed better at low temperatures with high alcohol yield. At 20±2°C, the fermentation was dominated by the growth of S. cerevisiae in Rhodomyrtus tomentosa juice with maximum ethanol concentrations (13.43%Vol.) The methanol and SO2 concentrations met the Vietnamese Standards (QCVN 6-3 2010/BYT). In addition, the total acid, ester and aldehyde concentration were also low.
Assessment of the Growth and Development of Daylily(Hemerocallissp.) Genotypes in Gia Lam, Hanoi

Pham Thi Minh Phuong, Pham Thi Bich Phuong, Nguyen Anh Duc, Nguyen Xuan Truong

Received: 05-06-2022 / Accepted: 27-09-2022
This study aimed to select new desirable genetic materials in terms of plant growth and shape, size and color of flowers for decorative daylily development program in Vietnam. Sixteen hybrid lines and three cultivars were evaluated. Each genotype was planted with 15 clusters and 2 stems for each cluster. Plant height, number of leaves per stem, flower scape size, number of buds per plant, flower sizewere recorded. All lines/cultivars showed good growth and development in Hanoi’s climate condition. The plant height ranged from 46cm (183-9) to 116.3cm (133-13), scape height was from 38.7cm (193-9) to 110cm (133-5), and flower diameter was from 12.5cm (NB2) to 18.5cm (133-13). Their flower color was grouped into three categories:red, yellow and orange with varried patterns on the flower petals. The flower scape durability last from 7 days (188-1) to 21 days (133-3), resulting in cluster durability ranging from 12 days (NB2) to 31 days (133-3). Based on the growth and development, 12 best lines/cultivars, 193-3, 183-4, 188-1, 133-3, 133-8, 133-10, 133-13, K15, K16, 133-12, NN03, and KC19 were selected.
Genetic Identification of Target Mutationsin OsSWEET14 Promoter-edited TBR225 Rice Lines

Tran Lan Dai, Pham Thu Hang, Cao Le Quyen, Pham Thi Van, Pham Xuan Hoi, Nguyen Duy Phuong

Received: 29-11-2021 / Accepted: 05-04-2022
Understanding host-pathogen interactions is key in breeding disease-resistant crops, including bacterial leaf blight. As a key virulence strategy to cause bacterial leaf blight, Xanthomonas oryzae pv.oryzae(Xoo) secretes transcription activator-like effectors (TALEs) to control the expression of host target genes through binding to the effector-binding elements (EBEs) located on the promoter regions. Precise mutagenesis at the TALE-interaction sites on the host genes by using gene editing tools such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRSIPR/Cas9) has been seen as a promising way to improve BLB resistance ofrice cultivars. In a previous study, we generated some transgenic TBR225 rice lines containing a single copy of CRISPR/Cas9 expression construct for editing AvrXa7/Tal5EBE on the promoter OsSWEET14. In this study, we continued to evaluate genotype and phenotype of T1edited TBR225 lines to determinethe genetic stability and phenotypic effective of the CRISPR/Cas9-induced OsSWEET14mutations. The genotypic analysis showed that 65%of transgenic TBR225 plants harbored the targeted OsSWEET14mutation, including nucleotide (Nu) insertion and deletion. The OsSWEET14mutations were stably inherited to the next generation with an expected segregation ratio consistent with Mendelian segregation (1:2:1). The preliminary phenotypic analysis suggested that the mutation of OsSWEET14promoter did not affect the growth and yield of gene-edited rice plants. Some T1TBR225 lines showed the resistance to Xoo isolate VXO_11 in the leaf-clipping experiment. The obtained results are a premise for development of the BLB-resistant TBR225 rice variety in the future.
Heterosis and Combining Ability for Yield and Quality of Some Purple Waxy Corn and Sweet Corn Inbred Lines

Nguyen Thi Nguyet Anh, Nguyen Trung Duc, Vu Van Liet, Pham Quang Tuan

Received: 25-10-2021 / Accepted: 01-03-2022
This study aimed to assess the combining ability for yield and quality of purple waxy corn and sweet corn. A full diallel cross of six corn inbred lines (three purple waxy lines and three yellow sweet corn lines) was employed in 2020 Autumn-Winter season. Six parental lines and thirty F1hybrids were evaluated in 2021 Spring season at Vietnam National University of Agriculture. The field experiments were arranged in a randomized complete block design, with three replicates. The ratio of GCA/SCA less than 0.5 in most traits suggested that the non-additive gene action was more significant than the additive gene action for these traits. SCA is more important than GCA in high quality hybrid corn breeding. Reciprocal effects (REC) were found that the cross between purple waxy corn × sweet corn was more effective than the cross between sweet corn × purple waxy corn in terms of yield and quality traits. Seven hybrids that had potential commercialization with high SCA on yield-related traits and quality were identified, viz, THL1, THL2 (purple waxy), THL4, THL5, THL13 (purple waxy sweet), and THL20, THL30 (yellow sweet).
Breeding New Promising Mutant Rice Lines by Gamma Co60

Nguyen Thi Mien, Nguyen Trong Khanh, Tran Van Quang

Received: 05-08-2021 / Accepted: 09-12-2021
This study was carried out to evaluate agronomic characteristics, yield, and quality of inbred rice lines that have been selected from the segregated M2populations derived from gamma (Co60) irradiation on imported rice varieties. The field experiments were conducted at Field Crops Research Institute, Haiduong province, Vietnam. Out of 20 inbred lines, there are three promising lines have been selected with good agronomic characteristics such as: short growth duration, low plant height, light pest infestation, high yield, high ratio of milling yield, and low amylose content. The new line NN1-2-6-55, which derived from gamma Co60irradiated segregating the population of accession rice NN1, had a desirable growth duration of 129 days in spring and 100 days in summer season, slight pest infestation, good lodging resistance, harvested yield of 6.75 tons ha-1in spring and 6.07 tons ha-1in the summer crop season with milling yield reached of 70% and preferred eating quality with an amylose content of 14%. Consequently, the improving of growth duration, plant height of import materials could be used gamma radioactive Co60 for mutation.
Study on Expression of Chicken Interleukin 2 Recombinant Protein and its Modulate Cytokines Production in Macrophage Cell Line

Truong Anh Duc, Tran Thi Thanh Ha, Nguyen Thi Huyen, Chu Thi Nhu, Nguyen Thi Chinh, Hoang Van Tuan, Ly Duc Viet, Dang Vu Hoang, Yeong Ho Hong

Received: 20-11-2019 / Accepted: 16-06-2020
Interleukin-2 is a multifunctional cytokine, which modulates the proliferation and differentiation of various types of cells and induces cytokines expression. In this study, chicken IL-2 will be cloned, expressed and its functional characterization was evaluated in Macrophage (HD11) cell line. Genes coding for IL-2 (AF000631 from GenBank) linked with Trx-Tag thioredoxin leader and restriction sequences of EcoRI (5’end), HindIII (3’end) were cloned and then ligated into a cloning véc tơvector pCR2.1-TA. Chicken IL-2 gene was incorporated into pET32a(+) at restriction sites for the expression of the IL-2 gene in E. coliBL21. Chicken IL-2 recombinant protein produced in E. colisystem showed that it did not produce cytotoxicity and enhanced the proliferation of macrophage (HD11) cell line and induced IFNγ, IL-1β và and IL-6 mRNA expression. These results showed that recombinant chicken IL-2 protein production in E. colisystem was possessed natural biological properties and was recognized by specific antibodies against IL-2.
Effects of Some Technological Parameters on Drying Time and Specific Energy Consumption for Cassava Dryingin Continuous Cross-flow Particle Dryer

Nguyen Tuan Anh, Nguyen Dinh Tung, Le Minh Lu

Received: 07-06-2018 / Accepted: 17-08-2019
This article presented the results ofexperimental research on the effects of some technological parameters on drying regime (drying time and specific energy consumption) for cassava chooped into 3-5 cm in size on continuous cross-flow particle dryer. Throung an empirical study, the drying temperature was distributed in the range 75-95C and the drying velocity between 1.65-2.25 m/s. The use of experimental planning with three inputs (the drying temperature, the velocity of drying flow and the moisture content of the input material) to affect two target functions, viz.: minimum drying time and lowest specific energy consumption, revealed optimum technological parameters, as well as: drying temperature (87.52C),velocity of drying agent flow (1.9 m/s), moisture content of input material (58.68%) for the objective function. The drying time was 47.8805 hours and the specific energy consumption was1.18475 kWh/kgH2O.
Improving the Sweetness of Waxy Corn Lines Using Backcross Breeding

Pham Quang Tuan, Nguyen The Hung, Nguyen Viet Long, Vu Van Liet, Nguyen Trung Duc, Nguyen Thi Nguyet Anh

Received: 09-04-2018 / Accepted: 18-06-2018
Backcrossbreedingwas used with the aim to improve the sweetness of purpleand white waxy corn materials at VietnamNational University of Agriculture. The F1 ofspring between waxy corn with sweet corn was backcrossed with waxy corn for two generations (BC2F1). The backcrossing progeny underwent 3 generations of selfing to BC2F4 to recover the waxy corn genetic background. Evaluation and selection of BC2F4 waxy-sweet corn were done in 2017 spring season in Gia Lam, Hanoi. Phenotypic data were measured for growth and developmental characteristics, yield and yield components, total anthocyanin content, pericarp thickness, sugar content and eating quality. Elite corn lines were selected based on selection index computed from ideotype plant analysis involving 10 studied traits. Twenty best performing elite lines were identified for use in inbred line development program. Backcrossing method has remarkably improved the sweetness of waxy corn by 0.3 to 4.0 percent. Backcrossing method, therefore, can be used to enhance the sweetness of waxy corn in Vietnam.
Nghiên cứu khả năng sinh trưởng, năng suất và chất lượng củamột số dòng, giống lạc trong điều kiện hạn và thiếu đạm

Nguyen Thi Thanh Hai, Dinh Thai Hoang, Vu Dinh Chinh, Nguyen Viet Long

Received: 25-01-2016 / Accepted: 05-05-2016
Nghiên cứu được tiến hành trong vụ Xuân 2015 nhằm đánh giá khả năng sinh trưởng, năng suất và chất lượng của một số dòng, giống lạc trong điều kiện hạn nhân tạo trên đất nghèo hữu cơ và đạm, Khoa Nông học, Học viện Nông nghiệp Việt Nam. Bảy giống lạc năng suất cao được phát triển từ tập đoàn giống nhập nội năm 2011 và giống đối chứng Đậu Giấy (giống địa phương thích ứng với đất nghèo dinh dưỡng) được bố trí theo kiểu khối ngẫu nhiên hoàn chỉnh (RCBD), với ba lần nhắc lại. Gây hạn bằng cách ngừng tưới trong 45 ngày từ 30 đến 75 ngày sau gieo, sau đó tưới trở lại đến khi thu hoạch. Phân bón (trừ đạm) được bón như quy trình thông thường. Kết quả cho thấy trong điều kiện hạn và nghèo dinh dưỡng đạm, các dòng, giống lạc sinh trưởng tốt và chống chịu tốt với một số loài sâu, bệnh chính, có năng suất cao hơn giống đối chứng. Nghiên cứu này đã xác định được dòng D18 và giống Tainan 9 là những dòng giống tốt nhất có năng suất hạt và hàm lượng dinh dưỡng cao. Đây sẽ là những giống lạc tiềm năng phục vụ phát triển sản xuất tại các vùng đất khó khăn tại Việt Nam.
Xác định vị trí và chỉ thị phân tử liên kết với gen qui định tính bất dục đực trong dòng lúa TGMS 103S

Vu ThiThu Hien, Atsushi Yoshimura

Received: 10-09-2014 / Accepted: 21-04-2015
Dòng mẹ bất dục đực TGMS 103S sinh trưởng phát triển tốt trong điều kiện sản xuất hạt lai ở miền Bắc Việt Nam. Đây là dòng mẹ của một số giống lúa lai của Việt Nam như Việt Lai 20, Việt Lai 24. Gen điều khiển tính trạng bất dục đực của dòng TGMS 103S chưa được xác định. Do đó, nghiên cứu này sử dụng chỉ thị phân tử để xác định gen điều khiển tính trạng bất dục đực của dòng TGMS 103S. Thí nghiệm sử dụng phân tích kiểu hình và kiểu gen ở thế hệ F1và F2 của cặp lai TGMS 103S với R20. Sử dụng phân tích liên kết bằng phần mềm MAPMARKER. Kết quả đã xác định gen tms-103Snằm trên nhiễm sắc thể số 2 và liên kết với các chỉ thị SSR RM3294, RM6378, RM7575và RM71. Những chỉ thị này có thể sử dụng trong chọn giống lúa lai cho chọn lọc MAS tính trạng bất dục đực.
Method CalculatingEnergy Losses for Medium Voltage Grid Base on R.M.S Current

Received: 13-05-2014 / Accepted: 18-07-2014
The article presented the method of energy loss calculation for the medium voltage transmission grid based on root mean-square current. Although the energy loss for the medium voltage transmission grid has been studied by many authors and proposed approximate calculation methods, it is inadequate for the application of such methods for concrete condition of the medium voltage grid in our country. By using statistical probability theory and the concrete condition of the medium voltage grid, the energy loss calculation method prốped semed to be conveniently and accurate in collecting data required..
Identification of Drought-tolerant Lines and Local Cultivars for Development Genetic Material and Rice Breeding for Rainfed Environment

Nguyen Thi Hao, Dam Van Hung, Pham My Linh, Vu Quoc Dai, Le Thi Hau, Dong Huy Gioi, Vu Van Liet

Received: 02-02-2013 / Accepted: 18-04-2013
Drought is a major constraint affecting rice production, especially in rainfed areas of mountainous regions of Vietnam. Development of drought tolerant varieties will improve rainfed rice production. Identification of drought-tolerance of the lines and local cultivars is useful for the development of genetic material and rice breeding for adaptation to rainfed environments. Forty two rice accessions were evaluated for germination and seedling characteristics by treatment of rice seed with KClO3. The plants were planted in PVC pipe to evaluate root length and root growth under well-watered and water-stressed environments. In addition, QTLs associated with root lengthen and root growth were analyzed using SSR markers. We have identified 11 accessiopns (lines and local cultivars) with drought tolerance. Three best drought tolerant accessions, 455 (Khau li on/Q5), 464 (Mua chua, Dien Bien) and 487 (Khẩu lếch, Bak Kan) are recommended for rice breeding program for rainfed environment.
Relation between Photosynthesis with Individual Yieldand Quality of RiceLines

Ngo Thi Hong Tuoi, Doan Kieu Anh, Quyen Ngoc Dung, PhamVan Cuong, Nguyen Van Hoan

Received: 08-04-2013 / Accepted: 19-06-2013
The study wasconducted attheDepartment ofPlantGeneticsandBreeding, Faculty of Agronomy, Hanoi University of Agricultural inthe springandharvestin 2012to assess thephotosynthetic capacityofthelinequality riceandfertilizerofthe relationship betweenphotosynthesisforriceyieldandquality. Experimental resultsshow thatthe intensity ofphotosynthesiscorrelatedwithSPADindexinthreestages:tillering(r=0,98), flowering(r=0,79) andninewax(r=0,97). The intensityof photosynthesisiscorrelatedwithindividualperformance(r=0,84) instageninewaxandnotcloselyrelatedindicatorsof quality.Fromthe experimental resultsT1, T3andT13line are thecurrentprospectsfor thebreedingof high quality rice.
The Results of Breeding New Aromatic Thermosensitive Genic MaleSterile (TGMS) Line in Rice

Tran Van Quang, Tran Manh Cuong, Nguyen Thi Hao, VuQuoc Dai, Pham My Linh, Dam Van Hung

Received: 12-03-2013 / Accepted: 18-06-2013
The new E15Sline is thermosensitive genic male sterile line thatwas selected from 135S/Hoasuacrossingby pedigree method. The E15S has short growth duration, leaves numbers 14.1 and good phenotype such as: good tillering, stem and violet awn, long grain. The leaves and endosperm of E15S have aromatic smell mark 4. The critical sterility inducing point (CSIP) of E15S is 240C and high outcrossing ratio. The F1 was crossed between E15S and pollinators which have short growth duration, high yield, good quality and moderated resistance for disease and insects. The results of evaluation on F1 was selected E15S/Huongcom combination that has short growth duration (128 days), high yield (7.4 tons-1ha), milling rice 71.2%, head rice recovery 58.0%, long grain 7.1 mm and aromatic smell mark 3.8.
Evaluation of Apparent Nutrient Digestibility of Diets UsingFish Meal and Catfish By- Product Meal for Guinea Fowls

Nguyen Dong Hai , Nguyen Thi Kim Dong

Received: 15-03-2016 / Accepted: 17-08-2016
The experiment was carried out to evaluate the apparent nutrient digestibility of Guinea fowls at 8 and 10 weeks of age fed diets containing twodifferent protein feed sources and fourcrude protein (CP) levels. The trial used a completely randomized design with two factors and 3 replications. The first factor was two protein feed sources including fish meal and catfish by- product meal and the second factorcomposed offour dietary crude protein (CP) levels of 16, 18, 20 and 22%. The results showed that the apparent EE, NDF and ADF digestibilities of the diet with fish meal were higher than those of catfish by- product meal diet (P< 0.05). The diets contained 20% CP and 18% CP fed Guinea fowls at 8 and 10 weeks of age had the higher DM, OM, EE, CF, NDF, ADF digestibility coefficients and better N retention (P< 0,05).
Land use Recommendation for Development of Sugar Apple (Annona squamosaL.)in Dong Trieu Town, Quang Ninh Province

Nguyen Ngoc Hong, Tran Trong Phuong, Nguyen Dac Nhan

Received: 01-02-2023 / Accepted: 18-04-2023
The study aims to assess the suitability of the land and propose the use of the land for the cassava growing area to serve the development of the crop-growing area for Dong Trieu town, Quang Ninh province. At present, Dong Trieu town has 956.8ha of custard-apple trees, which have been verified for the effectiveness of this product. The land suitability assessment method is used to find the suitable area in administrative units for the custard apple tree. The study investigated and surveyed within 7 communes and wards (Binh Duong, Binh Khe, Trang Luong, Viet Dan, Tan Viet, An Sinh, Trang An) which are localities where households are developing planting models. na. From the map of land units along with the growth requirements of custard apple, the results have determined that there are 3,555.28ha at an appropriate level for na and 317,441.96ha at a less suitable level in the whole town of Dong Trieu as a basis. to propose an increase of 105.3ha of custard apple cultivation area by 2030 in An Sinh, Binh Khe, Trang Luong and 37.6ha in some other localities.

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