Search

Search Results

##search.searchResults.foundPlural##

In VitroAnti-Bacterial Activity of Herbal Extracts against Staphylococcusspp. and Streptococcus spp. Isolated from the Metritis in Cows

Nguyen Thi Thanh Ha, Nguyen Thanh Hai, Nguyen Nam Phuong, Nguyen Van Thanh

Received: 18-04-2017 / Accepted: 26-07-2017
The use of antibioticsfor the cow metritis treatment has many advantages, however, raises concerns about antibiotic residues in the meat and dairy products as well as increasing antibiotic resistance of pathogenic bacteria. Herbs have been increasingly demonstrated their significant role in the pharmaceutical industry as a biosafety solutionandan alternative to synthetic chemical drugs. The present study aimed to examine the anti-bacterial activity of herbal extracts(Polyalthia longifolia var.Pendula, Lignum Caesalpiniae sappari, Excoecaria cochinchinensis Lour.,Clerodendron fragrans Vent, Wedelia calendulaceae Less, Cudrania tricuspidata, Pseuderanthemum (Ness)Radik, Lactuca indica L)against Staphylococcusspp. and Streptococcusspp.isolated from cow metritis. The resultsshowed that the extractionefficiency varied from 9.60% (Lignum Caesalpiniae sappari) to 14.32% (Wedelia calendulaceae Less). At the concentration of 100 mg/ml, all the extracts showed good anti-bacterial activity against Staphylococcusspp. and Streptococcusspp. For Streptococcusspp., the bacterial inhibition zone varied from 11.80 mm (Cudrania tricuspidata(Carr) to 25.00 mm (Clerodendron fragrans Vent). The diameter of the bacterial inhibition zone of Staphylococcusspp. varied from 10.28 mm (Cudrania tricuspidata(Carr)) to 25.30 mm (Wedelia calendulaceae Less). The extract solution of Clerodendron fragransVent and Wedelia calendulaceaeLess showed the highest anti-bacterial activity. This extract remained its anti-bacterial activity against Staphylococcus spp. and Streptococcusspp. at concentration of 0.20mg/ml.
Isolation and Antibiotic Susceptibility of Flavobacterium columnare Infecting Grass Carp Cultured in Northern Vietnam

Truong Dinh Hoai, Doan Thi Nhinh, Tran Thi Trinh, Dang Thi Hoa, Nguyen Ngoc Tuan, Kim Van Van

Received: 23-02-2022 / Accepted: 05-04-2022
This study was conducted to identify the pathogen F. columnarefrom diseased grass carp cultured in northern Vietnam and to examine the susceptibility of this bacterium to various common antibiotics used in aquaculture. A total of 103 grass carp samples suspected to be infected with F. columnare were collected and screened from 25 grass carp farms in Ha Noi, Hai Duong and Bac Ninh provinces. The grass carps infected with F. columnareexhibited the symptoms of decayed, white-grey gill filaments, rotten fins, and skin discoloration. From 67 yellow and rhizoid isolates recovered from the collected samples, 25 representative isolates (one per farm) were identified by the phenotypic tests and PCR assays. Grass carps (20-25g) challenged with F. columnareat the dose of 2 ×106 CFU/ml by immersion in 2h resulted in 100% mortality within 48-72 hpi. Theantibiotic test showed that high proportion of F. columnareisolates were susceptible to doxycycline, amoxicillin, florfenicol, and oxytetracycline (92-96%), but resistant to sulfamethoxazole/trimethoprim (44%) and trythromycin (16%). The present study provided necessary information for diagnosing and controlling F. columnarein grass carp.
Improvement of the Efficiency of Bovine in vitroFertilization Embryo Production

Do Thi Kim Lanh, Nguyen Thi Ngoc Anh, Hoang Thi Kim Chi, Nguyen Thi Hong Nhung, Nguyen Ba Truong, Su Thanh Long, Nguyen Van Thanh

Received: 18-08-2020 / Accepted: 07-12-2020
The present study was conducted to improve the efficiency of in vitroproduction of bovine embryos. Bovine oocytes were matured either in BO-IVM or TCM199 medium then evaluated the maturation rates by orcein staining. After maturation, bovine oocytes were in vitro fertilized (IVF) in a medium with frozen-thawed sperms at different concentrations (1, 2, or 5 ×106sperms/ml) for 12 hours. The fertilization process was then evaluated at 3, 6 or 12h of oocytes and sperms co-incubation to determine the optimal sperm concentration and co-incubation time. Denuded zygotes were then transferred into SOF medium with or without pre-cultured cumulus cell to evaluate the cleavage rates at day 3 and blastocyst formation rates at day 7. There was no significant difference in the maturation rate of bovine oocytes cultured in BO-IVM or TCM199. Fertilization of bovine oocytes with frozen-thawed sperms at 2 ×106sperms/ml for 6 h significantly improve the fertilization rate and blastocyst formation rate (69.87% and 29.1%). The cleavage rate and blastocyst rate of cattle fertilized oocytes cultured in SOF medium with pre-cultured cumulus cells were higher than those of embryos cultured without cumulus cells (76.34% and 34.16% vs 54.23% and 27,22%, P <0.05). The optimal conditions for in vitro produced cattle embryos can be performed as the maturation of the oocytes with TCM199 medium, fertilization at 2 ×106sperms/ml for 6 hours, then cultured in SOF medium with pre-cultured cumulus cells.
In VitroAnalysis of Mitochondrial Unfolded Protein Response and Expression of HSPA9, HSPD1 and ATF5 using Cell Culture Models

Thanh Trung Nguyen, Yuki Kambe, Nguyen Van Giap, Mai Thi Ngan, Vu Thi Thu Tra, Huynh Thi My Le, Bui Tran Anh Dao

Received: 17-04-2023 / Accepted: 20-11-2023
This study aimed to assess the role of the mitochondrial chaperones signaling factors induced by the mammalian mitochondrial unfolded protein response. In vitromodels were used with the mRNA of Hspa9, Hspd1 and ATF5 gene measured upon mitochondrial stress via Paraquat and 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid in NIH3T3 and HEK293 cell lines by quantitative real time Polymerase Chain Reaction. The results demonstrated that NIH3T3 cells were not suitable as a readout for UPRmt induction in vitro. Further, it was found that the levels of the transcription factor ATF5 tended to be decreased, while the upregulation of the Hspa9 and Hspd1 was observed upon mitochondrial stress in the context of the cells treated with 50 or 500μMParaquat or 3μM2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid. Notably, glucose concentration directly affected mammalian mitochondrial unfolded protein response signaling in vitro. Taken together, the results of the study indicates that the HEK293 cell line should be used to evaluate the expression levels of the genes upon mitochondrial stress conditions and might pave the way for the development of novel therapies for the cure of mitochondrial disorders and associated diseases in the future..
Evaluation of Effectivenessof Fecal Examination Techniques for Parasite Eggs and Oocystsin Dogs

Nguyen Thi Hoang Yen, Nguyen Than Thien, Dang Thi Phuong Thao, Nguyen Duyen Tung, Ha Quoc Viet

Received: 18-04-2019 / Accepted: 28-08-2019
The aim of this study was to evaluate the effectiveness of three fecal examination techniques: sugar floatation (SG: 1.27), saturated salt flotation (SG: 1.18) and formol-ether concentration, to determine the parasite eggs and oocysts in dogs. Examination of 270 dog fecal samples in some areas of Vinh Phuc, Thai Binh, Phu Tho and Thai Nguyen provinces, the result showed that the overall prevalence of gastrointestinal parasites in dogs is 75.18%. Twelve kinds of parasites were detected, consisting of one intestinal fluke (Heterophyids), six roundworms (Toxocara canis, Toxascaris leonina, Trichuris vulpis, Capillariasp., Strongyloides canisand hookworm), four tapeworms (Dipyllidium caninum, Diphyllobothrium latum, Spirometrasp., and Taeniasp.) and one coccidia (Cystoisosporasp.). Hookworm (58.81%) is the most prevalent in dogs, following by and Toxocara canis(31.11%). Sugar floatation technique showed the most effective for detection of roundworm eggs and coccidia oocysts, following by saturated salt floatation technique. Formol-Ether concentration one has more effective for detection of tapeworm egss and larva-containing roundworm eggs compared to the other techniques, but it cannot detect coccidia oocysts. In conclusions, the result of study showed that the choice of which fecal examination technique depending on the purpose of each study.
Management of Small and Medium-Sized Enterprise Income Tax in Bao Thang District, Lao CaiProvince

Le Phuong Nam, Le Kien Quyet, Nguyen Van Song, Nguyen Thi Bich Hang

Received: 27-03-2024 / Accepted: 24-06-2024
The enterprise income tax plays a crucial role in the economic and social development of Bao Thang district. However, enterprise income tax management is grappling with several challenges, including the following: ineffective promotional campaigns, a high rate of tax finalization dossiers containing underreporting or incorrect declarations aimed at reducing or evading enterprise income tax, a concerning increase in the number of non-compliant enterprises, and a rising amount of outstanding enterprise income tax arrears. This study aimed to assess the status of managing small and medium-sized enterprise income tax, analyze influencing factors, and propose solutions to enhance the management of corporate income tax in Bao Thang district. The research involved a survey of 30 officials and leaders from the Tax Department and 60 small and medium-sized enterprises. Data were analyzed using descriptive and comparative statistical methods. The results indicate that from 2020 to 2022, outreach and support activities for taxpayers have improved, featuring diverse and practical content. Debt management for the corporate income tax was accurate and flexible, providing favorable conditions for business operations. Tax inspections were conducted timely and effectively, simultaneously identifying and addressing violations without encountering complaints or accusations related to corporate income tax. Proposed solutions include strengthening outreach efforts, refining tax registration and declaration processes, improving debt management practices, conducting thorough business inspections, and enhancing the professional competence of the tax officer team.
Epidemiology Characteristics of Canine Distemper Diseases in Dogs in Hanoi

Nguyen Thi Huyen, Pham Ngoc Thach, Nguyen Vu Son, Nguyen Huu Nam

Received: 14-03-2019 / Accepted: 10-06-2019
This study aimedto determine the prevalence of Canine distemper disease in Hanoi as an important information for disease prevention. An epidemiological study on 18244 dogs was conducted in some veterinary clinics in Hanoi from 2013 to 2018. The clinical diagnosiswas based on the specific clinical signs. Treatment and clinical outcome were followed after confirming by CDV Ag test kit.The study found that575 (3,15%) dogswereinfected with Canine distemper virus (CDV) in 14 popular dog breeds with the typiclcal gastrointestinal, respiratory and neurological symptoms. Mong Coc breed hadthe highest positive proportion (6.32%), followed by Becgiebreed (5.56%), Corgi breed (4.28%), Alaska (4.27%). The lowest positive percentage (1.62%) was observed in Rottweiler breed. The infection and successful treatment percentage significantly differred from one year to another (P <0.05). Among age groups, dogsaged 2-6 months old hadthe highest CDV positive rate (60.35%), followed by group of 6-12 months old (15.13%), under 2 months old (12,35%) and lowest in over 12 months old dogs(12.17%). The prevalence of CDV among seasonsof winter, spring and autumn were 39.13%, 35.82% and 11.83%, respectively. The infected CDV dogs also had statistically significant difference in age and season of the year (P <0.05).
Single Nucleotide Polymorphisms (SNPs) inthe Gene Segment Coding for Cathepsin DAspartic Proteaseof Zoonotic Hookworm Ancylostoma ceylanicum

Duong Duc Hieu, Bui Khanh Linh, Nguyen Thu Huong, Le Duc Vinh, Nguyen Thi Hoang Yen, Nguyen Thi Hong Chien, Nguyen Van Phuong

Received: 02-04-2019 / Accepted: 01-06-2019
This study was conductedto determine the presence of the canine zoonotic hookworm species, Ancylostoma ceylanicum, in humans in Vietnam and to analyze the single nucleotide polymorphism in thegenesegment encoding A. ceylanicumcathepsin D aspartic protease, one of the hydrolytic enzymes with an important role in thelife cycle of hookworm. The existence of Ancylostoma ceylanicumin dogs and humans in Vietnam was confirmedby KOD-PCR. Comparative analysis of tthesequences encoding A. ceylanicumcathepsin D aspartic proteaserevealed, some mutations in exon 7, 8,and 9. Three SNPs non-synonymous mutations (one from exon 7 and two from exon 9)caused the changes in amino acid sequences of aspatyl protease.Our results can be considered forfuther studies on the development of effective vaccine against hookworm diseaseusing recombinant cathepsin D aspartic protease as an antigen.
Applying the Theory of Planned Behavior to Analyse Factors affecting the Adoption of Acacia Tissue Culture Derived Planting Materials in Plantation of Forest Households in the Quang Nam Province

Truong Quang Hoang, Ho Le Phi Khanh, Nguyen Thi Da Thao, Nguyen Thanh Phong

Received: 04-05-2023 / Accepted: 21-06-2024
This study was based on theory of planned behavior to investigate factors affecting the behavior on adoption of Acacia tissue culture derived planting materials of forest plantation households according to the certification of Forest Stewardship Council (FSC) based in Quang Nam province. The structural equation modeling (SEM) technique on SmartPLS 4.0 was applied to analyze the data from 171 FSC households in study site of three districts: Tien Phuoc, Hiep Duc, and Bac Tra My. The findings indicated that a minor 15.4% of FSC households applied tissue cultured derived planting materials for their plantation. The majority of FSC households have accessed acacia seedlings from local nurseries and companies in commune and district. Efficiency awareness of tissue culture derived planting material use andknowledge of FSC households on Acacia farmingexertedpositive viewtoward tissue culture derived planting materials. The viewpersonal standards, and perceived behavior control on the use of tissue cuntrure derived planting materials positively influencedthe intention to use in the nextcycle of acacia plantation.
Main Pathological Characteristics and Distribution of Classical Swine Fever Virus

Bui Thi To Nga, Kenji Kawashima, Bui Tran Anh Dao, Makoto Osaki, Nguyen Vu Son, Le Viet Hai, Tong Duc Thanh, Nguyen Thi Lua

Received: 19-04-2023 / Accepted: 29-08-2023
The aimsof this study wereto investigate the pathological features and tisue tropism of classical swine fever virus (CSFV) in the internal organs of the naturally infected pigs in some provinces inNorth Vietnam. Necropsy was performed on 8 CSFV infected pigs at differentagesto observe the gross lesions, collect specimens for histopathological study and detect viral antigen by immunohistochemistry. The gross findings included haemorrhages, inflamation in lymph system, spleen margin infarction, petechialor diffusehaemorrhages in various organs. The microscopic lesions weremainly hyperemia,haemorrhages, lymphoid deleption, necrotic and vascular cuffing in most internal organs. Immunohistochemical examination revealed thepresent of CSFV antigen in all 12 organs with high virus load in lymph nodes, spleen, lung and kidney. This is the first report about tissue tropisms of CSFV in the infected pigs by using IHC method inNorthVietnam.These results suggested the circulating virus was high virulent and induced acute disease form in the infected herds.
In VitroPropagation of Yellow-Flesh Taro (Colocasia Esculenta(L.) Schott) Collected in Thanh Son District, Phu Tho Province

Nguyen Thi Lam Hai, Pham Thi Thu Hang, Dinh Truong Son, Dang Thi Thanh Tam, Nguyen Thanh Hai, Do Thi Kim Ngoc, Nguyen Thi Thanh Thuy

Received: 10-06-2020 / Accepted: 22-09-2020
The yellow-fleshtaro belongs to the group of taro (Colocasia esculenta(L.)Schott)andis the native taro of Thanh Son district, Phu Tho province with high nutritional and economic value. The yellow-flesh variety is now being degenerated due to clonal propagation for many generations,therefore this study aims to rejuvenate this taro variety usingplanttissue culture. The mature tubers were used as a material to initiate in vitroculture.The in vitroshoots formed after the initial culturewerecut and culturedin MS medium supplemented with the different plant growth hormones for rapid shoot multiplication. The shoots were subcultured to MS medium supplemented with growth regulators belonging to the auxin group for rooting. The results showed that the optimal medium for rapid shootmultiplication was MSmedium supplemented with1.0mg/L BAP which would multiply 9.65 shoots/sample. The rooting medium for in vitroshoots was MS mediumsupplementedwith0.3 mg/L α-NAA with a rooting rate of 100%. The in vitroplants weretransferred to the greenhouse and grown in soil substrate and coconut fiber in 1: 1 ratio, the survival rate was 90%, the plantlets showed vigorous growth and produced new leaves after 2 weeks of acclimatization.
Ethnic Minority Freelance Workers in the Construction Sector in Hanoi: Migration Motivation, Employment and Difficulties and Challenges

Do Thi Diep, Nguyen Minh Duc, Ta Van Tuan, Le Tien Tung, Nguyen Thao Huong, Nguyen Duc Ninh

Received: 27-03-2024 / Accepted: 12-06-2024
Migrant labor of ethnic minorities in Vietnam has become a common phenomenon in recent years.. This study focused on researching the issues of migrant workers in the informal sector in the construction sector in Hanoi. Using a qualitative, participatory analysis method, the findings of this study show that the migration motivation of ethnic minority groups was mainly job opportunities and income. The freelance ethnic migrant workers in the construction sector faced difficult working conditions, minimal living conditions, various risks without protection, and challenges in integrating their life in Hanoi.
Design and Manufacture of Model for 3-Axis CNC Mini Milling Machine

Nguyen Thi Thu Trang, Pham Thanh Cuong, Tong Ngoc Tuan, Ngo Dang Huynh, Nguyen Huu Huong

Received: 23-10-2018 / Accepted: 22-01-2019
The designand manufacture fora model of a 3-axis CNC milling machine were made based on theoretical and empirical research. The machine model is usedmainly in the training and scientific research. Using this model, it is possible to fabricate many kinds of details byusingMACH3 software on the computer for control and simulation. The technicalsoftwareis used to produce detailed design drawings and overall model of the machine,followed byfabricatingthe parts and assemblingthem into a complete machine model.
Isolation and Identification of Soft Rot Bacteria on Dendrobium AnosmumOrchid and Evaluation of Inhibitory Effects of Some Actinomycetes

Pham Hong Hien, Diem Dang Truong, Duong Van Hoan, Tran Thi Dao, Nguyen Thi Thu, Nguyen Xuan Canh

Received: 20-07-2021 / Accepted: 09-12-2021
Bacterial soft rot is one of the most common diseases and mainly affects plants including orchid Dendrobium anosmum. The bacteria causing soft rot penetrates plant tissue, causing soft rot through the wound. This study aimed to íolate and identify bacterial strains capable of causing soft rot disease on orchids and select actinomycetes capable of antagonizing pathogenic bacterial strains. From five samples of orchid leaves showing symptoms of soft rot disease collected in Bac Giang, two strains of bacteria capable of causing soft rot disease were-isolated and the pathogenicity determined by artificial re-infection method. Bacterial strains were identified as Bacillus pumilusVK3 and B. altitudinisVK5B. By usingthe disk-diffusion method, from six Streptomycesstrains, XK7 Streptomycesstrain was selected for its antagonistic effect against B. pumilusVK3 and B. altitudinisVK5B-with the diameters of clear zone of17 ± 0,07mm and 14 ± 0,05mm, respectively.
Performance of Cooperatives in Tien Hai District, Thai Binh Province

Le Thi Thanh Hao, Vu Ngoc Huyen, Nguyen Van Song, Bui Thi Nga, Nguyen Quang Nam

Received: 20-04-2020 / Accepted: 08-09-2020
The study investigated 35 cooperatives and 105 members of cooperatives in Tien Hai district in order to collect the situation and to propose recommendations to improve the results of production and business activities of cooperatives in the district. The study investigated 35 cooperatives and 105 members of cooperatives in Tien Hai district in order to collect information on production and business activities of cooperatives. On the basis of analyzing the current situation, the study has proposed some recommendations to improve the results of production and business activities of cooperatives. The results showed that most of the cooperatives in Tien Hai are operated in many fields with two main business activities, production and service provision. In the period of 2016-2018, the average profit tended to increase but remained at low amounts. The profit was mainly used for reinvestment, without distributing to members. The majority of cooperative members must take the initiative in capital generation and product consumption. The mobilization of capital from the members is minimal. Members are satisfied with participating in the cooperative; the quality and price of products and services provided by the cooperatives are assessed at good levels. However, the role of cooperatives in helping members increase revenue, increase profits and the initiative to seek new ideas to increase the performance is only at a neutral score. In order to improve the performance of cooperatives in the district in the coming time, the authorities, cooperatives and members need to focus on: solving capital difficulties; promoting investment in consumption activities; strengthening linkages between businesses with cooperatives and members; and encouraging the cooperatives and members participate in safe agricultural value chains.
Application of the Different Non-linear Functions to Describe Growth Curve of Beef Cattle Crossbreds between Lai Sind and Belgian Blue

Nguyen Thi Vinh, Duong Thu Huong, Tran Bich Phuong, Ha Xuan Bo, Do Duc Luc, Nguyen Thi Nguyet

Received: 19-08-2020 / Accepted: 10-09-2020
This study aimed to determine the best model to explain the growth curve of F1(BBB x Lai Sind) bulls using non-linear function models such as Logistic, von Bertalanffy, Gompertz, Brody, and Negative Exponential. For this purpose, live weight records of a total 60 F1(BBB x Lai Sind) consisted of 30 bulls and 30 heifers, reared in Ba Vi district, Hanoi city were used. In order to select the best model, the coefficient of determination (R2) and parameters of ME, MSE, MAE, MPE, MAPE were utilized. Results showed that, in both bulls and heifers, von Bertalanffy and Brody functions had the highest R2 and lowest ME, MSE, MAE, MPE, MAPE; followed by Gompertz function. Logistic and Negative Exponential functions had lower values of the above parameters. Age and body weight at the inflection point ranged from 15.47 to 20.41 months; and 400.13 to 415.44kg for bulls; 10.05-14.29 months and 255-276.27kg for heifers, respectively. Therefore, von Bertalanffy function is recommended to use for prediction of live weight on the age of F1(BBB x Lai Sind).
Isolation and Selection of Trichodermaspp. Antagonists against Pathogens CausingAnthracnose in Mango

Pham Thi Ly Thu, Le Trung Thanh, Luu Thi My Dung, Ha Viet Cuong, Nguyen Duc Thanh, Nguyen Thi Hong Hai

Received: 12-07-2021 / Accepted: 29-10-2021
Mango is a key crop and provides high economic efficiency in the Mekong Delta. However, its yield and quality are seriously affected by anthracnose disease. In order to select Trichoderma spp. with high antagonistic activity against disease fungi, 17 soil samples were collected from healthy plots in Hau Giang and other provinces in the Mekong Delta. A total of 56 strains of Trichodermaspp. were isolated and 14 strains were selected for high antagonistic activity against fungi causing anthracnose. Among those, strains Tr.X1, Tr.X2, Tr.X3, Tr.X4, T1, M2 and Tr.X2, Tr.X3 exhibited the most antagonistic activity from 77.76-86.25% inin vitro,and 75.3-72.01% in the net house. The strains were identified as Trichoderma harzianum(Tr.X2) and Trichoderma asperellum(Tr.X3) and they have potential of biological control to anthracnose in mango.
Evaluation of Agro-Biological Characteristics of some Cassava (Manihot EsculentaCrantz) Materials in Field Conditions and Screening of the Resistance to CMV in Artificial Infection

Hoang Thi Thuy, Tran Thi Thanh Ha, Vu Thi Bich Hanh, Nguyen Van Ha, Duong Thi Loan, Vu Van Liet

Received: 11-02-2020 / Accepted: 11-08-2020
This study was conducted to evaluate some agro-biological characteristics of 14 domestic and exotic cassava materials in Gia Lam, Hanoi condition, and to evaluate the resistance of the materials through artificial infection experiments by the transplanting method. The material was grown in the field in a sequential experiment without repeating, tracking some agro-biological characteristics. The materials were havested after 8-11 months. The plant height of the materials ranged from 165.2-183.2cm, there are 8 materials with branching height. Most materials have only 1 stem/cluster, CM15, CM21 had 2 stems/cluster and CM60 had 3 stems/cluster. The number of tubers/tree varies from 4.3-15.8 tubers/tree. The highest weight of fresh tubers was obtained CM15 (5.0 kg/tree) and 2 control varieties (5.26-5.82 kg/tree). Using the tranplanting method, evaluated after 2 weeks, 40 successful transplanted trees of 13 materials were evaluated. Genotyping of these transplanted trees was checked by JSP1/JSP2 and JSP1/JSP3 primers detected the presence of ACMV in plants of 8 materials (CM2, CM3, CM8, CM15, CM20, CM60, KM140 and KM94). Assessing the level of infection after 3 months of transplantation, the group of resistant and highly resistant varieties were identified including CM15, CM16, CM21, CM60, CM88, CM50, CM61 and KM94.
Effect of Additional Materials and Additives on Quality of Soy Yogurt Fortified with DragonFruitJam

Tran Thi Dinh, Nguyen Thi Quyen, Nguyen Thi Thuy Nga, Vu Thi Huyen, Than Thi Huong, Nguyen Thi Hoang Lan, Daylan A Tzompa-Sosa, Koen Dewettinck

Received: 28-07-2023 / Accepted: 12-04-2024
The study aimed to investigate the effect of ingredients and foodadditives on the quality of soy yogurt. The quality attributes of soy yogurt with dragon fruit jam addition were determined using instrumental analysis. The sensory properties of the product were evaluated using the ranking method. The results showed that soy yogurt supplemented with 1.5% whey protein, 0.5%skimmed milk, 0.5% whipping cream, and stabilizers of 0.8% gelatin and 0.2% modified starch resulted in the soy yogurtwith protein content equivalent to animal-basedyogurt with 3.6% protein, 1.3% lipid, 12.0% carbohydrate, stable product texture,and highly acceptable sensoryquality.
Pathological Characteristics of Kept WildPigsInfected with Porcine Epidemic Diarrhea Virus (PEDV)

Nguyen Thi Thom, Nguyen Vu Son, Pham Hong Ngan, Nguyen Huu Nam

Received: 24-02-2017 / Accepted: 15-05-2018
Thirty-five piglets aged from 1 to 4 weeks were collected from wild pigfarms in Ha Giang province. The RT-PCR was used to confirm porcine epidemic diarrhea (PED) virus. The results showedthat the proportion of positive samples was74.3% and 54.3% for small intestine and mesenteric lymph node andfor fecal samples, respectively. The main clinical signs of PED included weakness, depression, and diarrhea with loose, fishy, and yellow stools (100%)andweight loss (80.8%). Suckling pigs showed anorexia, huddling together, laying on the sow (76.9%), dehydration (69.2%), loss of appetite (57.7%), hypothermia and heaving (53.8%).The autopsies showed distended stomach containing curd and undigested food (100%),thinness of the wall of small intestine and watery yellow translucent content in small intestine (100.0%),hyperemia and hemorrhage of mesenteric lymph nodes (80.8%),enlargement of lymph nodes in other organs (69.2%); pale liver (69.2%),pneumonia (61.5%),splenic congestion, and gallbladder enlargement (57.7%). Microscopic lesions included small intestinal congestion, hemorrhage, severe villous atrophy, scraped mucosa, lymphoid follicles hyperplasia, inflammatory cell infiltration, degeneration, and necrosis. Congestion and inflammatory cells infiltration were observed in liver, spleen, and lung. In addition, hepatocellular degeneration and lipid deposition were observed in the liver.
Enrichment of Protein inCassava Residuesby Simultaneous Saccharification and Fermentation

Duong Thu Huong, Pham Kim Dang, Tran Hiep, Ngo Thi Huyen Trang, Nguyen Thi Nguyet, Vu Van Hanh

Received: 06-03-2018 / Accepted: 08-05-2018
The aim of study was to improve the nutritional quality of cassava residues by means of simultaneous saccharification and fermentation. Cassava residues were mixed with water at the rate of 30% and saccharification was carried out with crude enzymes (α-amylase, glucoamylase, cellulase) at different concentrations (0, 2, 4, 6, 8, 10%). After 24 hours, at 8 and 10% crude enzymes, the reducing sugar was highest while the starch and crude fiber content were lowest. Simultaneous saccharification and liquid fermentationof cassava residues were conducted in 3L fermenters supplemented with 8% enzyme, 1% (NH4)2SO4and S. cerevisiae, Lactobacillus sp., and Bacillus sp. (107cfu/ml of each) at room temperature. After 48 hours fermentation the crude protein increased by 7.3 times and real protein increased by 5.5 times compared with unfermented cassava residues, while HCN decreased to 20.54 mg/kg, organic acid increased by 5.9 times, pH value was 3.7. The fermented product had good flavor, slight acidity and no aflatoxin.
Optimizing Some Factors of Drying Tomato Waste for Lycopene Extraction

Nguyen Thi Hoang Lan, Nguyen Thi Kim Thanh, Nguyen Thi Quyen, Tran Thi Nhung, Nguyen Ngoc Cuong, Tran Thi Dinh

Received: 27-10-2017 / Accepted: 02-04-2018
Lycopene, one of the natural substances, possesses high antioxidant activity. It is widely used in food, cosmestic, and pharmaceutical industries. Tomato is a rich source of lycopene present in the water insoluble fractions such as skin and pulp. Therefore, solid waste from the tomato processing industry is a potential material for lycopene extraction. This study aimed to optimize factors influencing the drying process of tomato waste to be used for lycopene extraction. Optimal drying temperature and final moisture content were 65oC and 23%, respectively. Under these conditions, the lycopene content of the dried tomato waste was 8.92 mg/g dry matterand the antioxidant activity of the lycopene extract was8.14 µmol TE/g dry matter.
Maintaining Criteria of the New Rural Dvelopment Program in Phu Tho Province: Current Status and Recommendations

Nguyen Tuan Son, Nguyen Anh Duc, Dang Nam Phuong, Nguyen Thi Thu Huyen

Received: 05-05-2021 / Accepted: 20-07-2021
Many provinceshave completed the new rural development programand continuedto maintain and improve the quality of criteria in the program. However, maintaining the new rural criteria is still difficultdue to several reasons. This study assessedthe current situationand the results ofmaintaining new rural development programin Phu Tho province during2010-2020period. The study useda participatory approach to gather information and compared the results by the criteria and the periods. Based on the survey results, the study proposedsomerecommendations to continue maintaining and improving the criteria quality ofthe new rural development program in Phu Tho province.
Study on Micropropagation of Strawberry “Smia”

Nong Thi Hue, Pham Thi Thu Hang, Tuong Thi Thanh Huyen, Nguyen Thi Thuy Linh, Nguyen Thi Lam Hai, Nguyen Thanh Hai

Received: 20-09-2017 / Accepted: 31-01-2018
Strawberry “SmiA” is a potential variety with outstanding features such as heart- shaped fruit, dainty little red berries with an ambrosial flavor of strawberry, rose, pineapple and honey. This study was carried out to establish in vitro micropropagationprotocol from in vitro germinated plants. The best shoot proliferation (7.8 shoots/explant) was obtained from explants grown on MS medium supplemented with 0.4 mg/l BA after 5 weeks. In vitro shoots were rooted on semi-solid MS medium with 0.25 mg/l α-NAA, maximum rooting rate (93.33%) with 12.36 roots/shoot and average length (1.49 cm) of roots was achieved after 4 weeks. The plantlets were successfully acclimated and well developed in soil substrate with a high survival rate. NPK fertilizer combined with Atonik solution was most appropriate for growth and development of in vitro SmiA plantlets with the average height of 19.13 cm after 1 month. Flowering was observed after 20 - 25 days of flower-bud formation, and masses of dainty, sweet tasting berries were produced after 30 - 35 days.
In vitroPropagation of Gardenia jasminoides Ellis

Dinh Thi Thu Le, Bui Thi Hai Hoa, NguyenThi My Uyen, Doan Thi Thuy Hang, Ninh Thi Phip, Nguyen Xuan Truong, Pham Van Cuong, Dinh Truong Son

Received: 07-12-2022 / Accepted: 07-03-2023
Gardenia (Gardenia jasminoidesEllis) is not only valuable as a food but also a medicinal plant used in the treatment of illnesses including jaundice, headaches, fever, inflammation, hepatic disorders, and hypertension.The current study aimed to establish an efficient protocol for in vitropropagationof Gardenia jasminoidesEllis with high multiplication rate and healthy plants. The pre-mature Gardenia fruits were the most suitable plant parts for initiation of in vitroculture. Seeds sterilized using 0.5% Presept solution for 10 minutes gave the highest sterilization (94.67%) and germination rate (90.59%). MS medium supplemented with 1.5 mg/lbenzyladenine gave multiplication rate of 7.29 shoots/explant. MS medium supplemented with 30-40 g/l sucrose and 150ml of coconut water/l gave vigorous growth of microshoots and good shoot quality. MS medium supplemented with 0.5 mg/l IBA or half-strength of MS medium recorded100% rooting rate, normal root morphology, and well growing plants.

201 - 225 of 500 items << < 4 5 6 7 8 9 10 11 12 13 > >>

Search

Advanced filters

Search Results