Received: 02-10-2023 / Accepted: 26-01-2024
The main goal of study was to use nucleotide sequencing of the cytochrome c oxidase 1 (COI) gene to assess the genetic diversity of 9 bivoltine breeds and 5 native Vietnamese silkworm breeds. DNA Sequencing was carried out using by chain termination method. Data were analyzed by Bioedit, DNAsp and MEGA X softwareThe results indicated 17 polymorphic nucleotide substitution sites when comparing the COI gene sequence of the 14 silkworm breeds in this study with the sequence AB737913.1 on GenBank. Only one polymorphic nucleotide site appeared when comparing the 14 breeds with each other. These silkworm breeds were divided into 2 haplotypes, with a main concentration in haplotype 1. The genetic classification tree shows that 13 out of the 14 studied silkworm breeds were distributed in branch 2, which is the common branch of Chinese and European silkworm breeds. This outcome provides initial information for breeders to strategically use molecular markers in the conservation, exploitation, and development of the genetic resources of silkworm.