Received: 10-06-2020 / Accepted: 22-09-2020
The yellow-fleshtaro belongs to the group of taro (Colocasia esculenta(L.)Schott)andis the native taro of Thanh Son district, Phu Tho province with high nutritional and economic value. The yellow-flesh variety is now being degenerated due to clonal propagation for many generations,therefore this study aims to rejuvenate this taro variety usingplanttissue culture. The mature tubers were used as a material to initiate in vitroculture.The in vitroshoots formed after the initial culturewerecut and culturedin MS medium supplemented with the different plant growth hormones for rapid shoot multiplication. The shoots were subcultured to MS medium supplemented with growth regulators belonging to the auxin group for rooting. The results showed that the optimal medium for rapid shootmultiplication was MSmedium supplemented with1.0mg/L BAP which would multiply 9.65 shoots/sample. The rooting medium for in vitroshoots was MS mediumsupplementedwith0.3 mg/L α-NAA with a rooting rate of 100%. The in vitroplants weretransferred to the greenhouse and grown in soil substrate and coconut fiber in 1: 1 ratio, the survival rate was 90%, the plantlets showed vigorous growth and produced new leaves after 2 weeks of acclimatization.