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Evaluatiing the Effect of Organic Additives on the In Vitro Rapid Multiplicationof Musa acuminata

Dinh Truong Son, Dang Thu Hoa, Vu Cong Son, Nguyen Thi Hoa, Vu Thi Hien, Dang Thi Thanh Tam

Received: 27-03-2023 / Accepted: 22-05-2023
The study aimed to determine the effect of organic additives (coconut water, banana, or potato homogenates) on the in vitrorapid multiplication stage of the Musa acuminata (Cavendish subgroup). To test the effects of the mixtures, the multiplication media (MS + 2 mg/l Benzyladenine + 30 g/l sucrose + 6,0 g/l agar) were supplemented with different concentrations of organic additives. Treatments were compared based on multiplication rate, shoot length, and shoot morphology. At specific concentrations, all treatments with coconut water, banana, or potato homogenates had a positive effect on banana propagation. In comparison to the control (no organic additives added), multiplication media containing 40 ml/l coconut water or a combination of 20 g/l banana homogenate or 30 g/l potato homogenate were suitable medium to promote a higher multiplication rate. The propagation medium supplemented with 40 ml/l coconut water and 20 g/l banana homogenate exhibited the highest multiplication rate. Based on the finding this study, obtaibed results could be applied to the production of high-quality in vitro banana planlets.
Effects of Irrigation Mat and Substrates on Watering Cycle, WaterRequirement, Growth and Quality of Potted Dwarf Marigold(Tagele patula L.)

Nguyen The Hung, Nguyen Thi Thao, Le Phuc Binh, Pham Minh Phuong, Trinh Thi Mai Dung

Received: 22-08-2012 / Accepted: 21-09-2012
The use of irrigation mat made from textile materials is a new research trend for hi-tech agriculture. This technology has been using in developed countries such as Germany and the Netherlands. This paper summarized the study results on the effects of irrigation mat KT2 and three types of substrates on the watering cycle, the required amount of water, the growth and quality of dwarf marigold. The study composed of three experiments. The experiments were conducted with six treatments using the random complete block (RCB) design in the nethouse from January to June 2011. The experimental results indicated the following advantages of using irrigation mats: limitation of water loss, an appropriate water amount based on the demand of plant, long moisture retention of the substrates, extended watering cycle, and reduced water requirement and labor as compared with control treatment (no irrigation mats). Growing the plants using the substrate No.3 (alluvial soil, cattle manure, sooked rice husk and coconut fiber with the ratio 1.5:1:1:0.5) in combination with irrigation mat and normal irrigation methods gave better results than the substrate number 1 (alluvial soil) and 2 (alluvial soil, cattle manure and burning rice husk with the ratio 3:1:1).
DEVELOPING AN EFFICIENT REGENERATION PROTOCOL FOR SWEETPOTATO, Ipomoea batatas (L.) Lam., USING NODAL EXPLANTS

Vu Thi Thuy Hang, Ha Thi Anh Thu, Vu Dinh Hoa

Received: 06-09-2016 / Accepted: 03-11-2016
An efficient regeneration protocol of large scale micropropagation for clonal establishment ofsweet potato from seedlings is an essential step for clonal selection. The objective of thisstudywas to evaluate the potentials of young coconut water and simple aerated culture conditionin glass bottles with Milliseal®forin vitro propagation of sweet potato using nodal explants.Shoot regeneration of sweet potato nodes bearing dormant buds was effectively promoted by aerated culture condition in full strength MS salt solidified by 8g.L-1agar supplemented with 3% sucrose,BAP 1.0 mg.L-1,0.5 mg.L-1IAA and 20% coconut waterand subsequentroot induction was effective on full strength MS salt solidified by 8g.L-1agar supplemented with 3% sucroseand 0.3 mg.L-1α- NAA. The plantlets derived from aerated culture condition were more vigorous compared to conventional non-aerated culture condition, leading to high survival rate (~ 100%) and enhanced growth during acclimatiztion. Sand -smoked rice husk potting mixwas suitable for hardening of in vitro -derived plantlets.
Study on In vitroPropagation of Miltoniasp.

Phan Xuan Huyen, Hoang Van Cuong, Nguyen Thi Phuong Hoang

Received: 25-07-2014 / Accepted: 09-10-2015
In the present study, 3 -4 protocormsderived from meristem of red and white-pink Miltoniasp. were used as material source for all invitro culture experiments. Effect of MS medium concentration, coconut waterandBA alone or in combination with αNAA on protocorm formation and shoot growth were studied. After 90 days of culture, the results showed that half MS medium supplemented with 15% coconut water, 1.5 mg/l BA in combination with αNAA of 0.1 mg/l, 30 g/l sucrose, 8 g/l agar, 1 g/l activated charcoal,andpH 5.8 was the best for protocorm inductionand shoot growth (25.60 shoots/explant, height of 1.95cm). Half MS medium containing 0.1 - 1 mg/l αNAA or 0.1 - 1 mg/l IBA, 30 g/l sucrose, 8 g/l agar, 1 g/l activated charcoal,andpH 5.8 was suitable for in vitroroot formationafter 40 days of cultureandthe rate of shoot formation was 100%.Coconut fiber and fern fiber were suitablesubstratesfor plantlet growth in the greenhouse, with coconut fiber beingbetter in terms of number of roots/explant, root length andsurvival ratecomapred tofern fiber substrate.
Water-Pine (Glyptostrobus pensilis (Staunt) K.Koch) Conservation by In vitroGrafting Technique

Nguyen Thanh Sum, Dinh Thi Bich Thao, Nguyen Thi Phuong Thao, NguyenThi Kim Thanh

Received: 15-02-2016 / Accepted: 15-09-2016
Water-pine shoots (Glyptostrobus pensilis) derived fromin invitroculture of 8mm in sizewere grafted(whip grafting) onepicotylsof 30 day-oldChinese Fir(Cunninghamia lanceolata) plantlets. The Chinese firindividuals were produced by sowing seedson basalt and coconut fibermix with 7:3 ratioand 70%moisture The mix wasautoclaved at 1210C, 1 atm for 2 hours to sterilize. This grafting method producedcomplete invitrografted plants. They survived and grew well after transplanting tothe naturalconditions. The resultsmight contribute to research forconservation of a rare and endangered water-pinespecies.
Establishment of In vitroPropagation Protocol for Golden Camellia(Camellia sp.)

Dang Quang Bich, Nguyen Thi Phuong Thao, Nguyen Van Phu, Dinh Truong Son, Ninh Thi Thao, Nguyen Van Huan, Tran Van Lin, Nguyen Thi Thuy Linh

Received: 29-08-2017 / Accepted: 31-01-2018
Golden camellia (Camellia sp.)is a medicinal plant containing a wide range of pharmacologically active compounds that can be used in anti-inflammatory, prevention and treatment of cancer. Golden camillia is also used as an ornamental plant because of its bright yellow flowers. This study successfully established in vitro multipropagation protocol of Camellia sp. collected at Ba Che district, Quang Ninh province, Vietnam. Unopened fruits were identified as the best initial material. Unopened fruits were surface sterilized with 7% NaOCl solution for 30 minutes. Seeds were then placed on MS medium supplemented with 1 mg/l BA and 3 mg/l GA3 for germination. Shoots were multiplied on MS media containing 5 mg/l BA + 1 mg/l GA3 + 300 ml/l coconut water + 30 g/l sucrose + 7 g/l agar. The rate of shoot multiplication was 4.7 shoots per explant. The shoots were then transferred onto medium of 1/4 MS + 0.5 mg/l α-NAA + 2 mg/l IBA + 30 g/l sucrose + 7 g/l agar for rooting. At the nursery stage, in vitro plants were grown in sand and reached the survival rate of 93.75%. The established protocol can be applied for multipropagation and conservation of the golden camellia.
In vitropropagation of Taiwanese Sweet Mulberry

Nguyen Thi Ly Anh, Nguyen Thi Thiet, Tran Thi Luyen, Dang Thi Nhan, Nguyen Thi Han

Received: 26-07-2018 / Accepted: 03-12-2018
In vitropropagation of Taiwanese sweet mulberry was carried out to determine technical parametersinthe production of high quality planting materialsfor expanding cultivated area of the newly introducedfruit tree. One-month-old shoots of two-year-old Taiwanese sweet mulberry plantswere used as initial materialsfor in vitroculture. Results showed that BA at the concentration of 3mg/l in MS medium was suitable for stimulating in vitroshoots from stem nodes with highrate(83.33%). BA at 3 mg/l in combination with0.5 mg/l IBA and 10% coconut water gave the best shoot multiplication rate (2.93 shoots/explant) and the highest shoot quality after 4 weeks of culture. The highest rooting percentage at 96.67% of shoots was observed after 4 weeks onMS medium containing 0.5 mg/l IBA. The plantlets were successfully acclimated in sandy substrate with a survival rate of 80%. The liquid fertilizer “Buffalo Head 502” foliar spary 1 time/weekyielded good quality plantlets suitable for transplanting into pots.This in vitropropagation process is highly effective and easy to apply at production scale.
Effects of Organic Additives on Shoot Elongation of Dendrobium lituiflorum Lindl.

Dang Thi Thanh Tam, Tran Thi Thu Huong, Nguyen Thi Lam Hai, Nguyen Thanh Hai, Dinh Truong Son

Received: 10-06-2020 / Accepted: 12-12-2020
This study aimed to investigate the effects of adding several organic additives on the growth of the Dendrobium lituiflorum shoots. In vitro shoots of 1cm length were separated from protocorm-like body and cultured on the MS medium supplement with different organic additives such as homogenate of potato, germinated green bean, yam bean or banana pulp or coconut water with the concentrations of 50 mg/l or 50 ml/l, respectively. The results indicated that adding potato or yam bean homogenate promoted shoot elongation. Combination of 50 g/l potato homogenate with 10 g/l green bean homogenate or 50 g/l yam bean homogenate with 0.5 g/l active charcoal resulted in longer shoots. This is the first report on the effects of yam bean homogenate on orchid shoot development. The findings could be applied on a large scale of producing in vitro plantlets of Dendrobium lituiflorum.
In vitroPropagation of Gardenia jasminoides Ellis

Dinh Thi Thu Le, Bui Thi Hai Hoa, NguyenThi My Uyen, Doan Thi Thuy Hang, Ninh Thi Phip, Nguyen Xuan Truong, Pham Van Cuong, Dinh Truong Son

Received: 07-12-2022 / Accepted: 07-03-2023
Gardenia (Gardenia jasminoidesEllis) is not only valuable as a food but also a medicinal plant used in the treatment of illnesses including jaundice, headaches, fever, inflammation, hepatic disorders, and hypertension.The current study aimed to establish an efficient protocol for in vitropropagationof Gardenia jasminoidesEllis with high multiplication rate and healthy plants. The pre-mature Gardenia fruits were the most suitable plant parts for initiation of in vitroculture. Seeds sterilized using 0.5% Presept solution for 10 minutes gave the highest sterilization (94.67%) and germination rate (90.59%). MS medium supplemented with 1.5 mg/lbenzyladenine gave multiplication rate of 7.29 shoots/explant. MS medium supplemented with 30-40 g/l sucrose and 150ml of coconut water/l gave vigorous growth of microshoots and good shoot quality. MS medium supplemented with 0.5 mg/l IBA or half-strength of MS medium recorded100% rooting rate, normal root morphology, and well growing plants.
In vitroMicropropagation of Dendrobium officinaleKimura et Migo

Vu Ngoc Lan, Nguyen Tuan Anh, Nguyen Thi Hoa, Pham Tuan Anh, Nguyen Thi Phuong Dung

Received: 16-02-2024 / Accepted: 12-04-2024
Dendrobium officinaleKimura etMigoof the genus Dendrobiumis a rare and endangered orchid species. In vitromicropropagation was carried out for preserving and developing this precious orchid genetic resource. All experiments were arranged in a randomized complete design (RCD) with three replicates. The results showed that the fruit as initial material source produced 100% survival of explants, generatingprotocorn and shoots and superior to the initial material source of using buds (rate of survived explants was only 2-3%).The addition of coconut water to the culture medium did not have a clear effect on the morphogenensis of Dendrobium officinale.The MS medium was the most suitable forrapid multiplication of orchid shoot clusters (2.56protocorn/8weeks). Adding growth regulator -NAAat a concentration of 1ppm or BAP at a concentration of 3ppm to the basal medium produced the largest number of shoots (45 protocorn/8weeks) and high shoot multiplication coefficient (2.61protocorn/8weeks). For in vitrogrowth of orchid shoots, RE mineralculture medium supplemented with 0.1% activated charcoal was optimal for plantlet regeneration.
in vitroPropagation of Coelogyne mooreana Sander Ex Rolfe

H’Yon Nie Bing, Dang Thi Tham, Nguyen Thi Thanh Hang, Dinh Van Khiem, Nong Van Duy, Vu Kim Cong, Quach Van Hoi, Tran Thai Vinh

Received: 17-01-2016 / Accepted: 15-07-2016
In this study, in vitromass propagationof Coelogyne mooreanaSander ex Rolfe, an endemic orchid (of Vietnam) with its beautiful large,white flowers and fragrance having horticultural potential was accomplished through protocorm formation from shoot tips and subsequent plant regeneration. In the first procedure, shoot tips from in vitrogerminated seedlings were cultured on Murashige and Skoog medium (MS) supplemented with arange of 6- benzylaminopurine (BA) in combination with naphthalene acetic acid (NAA) to determine the best medium for the protocormformation. The results showed that MS medium supplemented with 1.0 mg/l BA and 0.5 mg/l NAA was the most suitable for protocorm formation (6.62 protocorms và formation rate of 86.63%). For subculture, maximum growth of shoots (14 shoots, 2.72 cm height and 3 leaves/shoot) was obtained on MS medium supplemented with0.3 mg/l NAA and 2.0 mg/l BA. The highest frequency of rooting (100%) was observed on ½MS medium supplemented with 20 g/l sucrose, 8 g/l agar, 1 g/l activated charcoal (AC),10% coconut water (CW) and 0.5 mg/l NAA. On this medium, the seedlings reached an average length of 1.66 cm with 3.8 roots per seedling. A successful protocol for micropropagation by shoot tips will contribute to the development of a suitable management program of C. mooreanaSander ex Rolfe.

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