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• Growth Performances of Mia Chicken Carrying Polymorphisms of the INS and GH genes

  Hoang Anh Tuan, Nguyen Hoang Thinh, Pham Kim Dang, Bui Huu Doan

  Received: 29-07-2021 / Accepted: 09-12-2021

   Abstract PDF (VIETNAMESE)

  This study was conducted to evaluate the growth performances of the Mia chicken carrying polymorphisms of the INS and GH genes. A total of 1,100 newly hatched of Mia chickens with characteristic morphological features, were selected from the Mia population in Son Tay town, and raised for evaluation of growth performance at Hadinco Farm. At 8 weeks of age, blood samples were taken from the wing veins of each individual and were identified the genotype by PCR-RFLP method.The results showed that the A3971G polymorphism of the INS gene had 3 genotypes AA, AG and GG with a frequency of 29%, 55% and 16%, respectively. The T3737C polymorphism had 2 genotypes CT (78%) and TT (22%); no CC genotype was found. The C423T polymorphism has 3 genotypes CC, CT and TT with frequencies of 21%, 45% and 34%, respectively. The polymorphism of G662A had 3 genotypes, in which genotypes AA and AG had frequencies of 51% and 40%, respectively. All of the above genotypes did not have a significant effect on the weight of Mia chickens, while those carrying GG genotype of the G662A had bodyweight higher by 10.4% in the general population. However, the number of Mia chickens carrying this genotype in the population was very low, accounting for only 9%.

• Evaluation of 24-bp Insertion-Deletion and C2402T Polymorphisms of the Prolactin Gene in Two Vietnamese Native Chickens: Riand Mia Chicken

  Nguyen Hoang Thinh, Nguyen Thi Chau Giang

  Received: 01-03-2018 / Accepted: 20-06-2018

   Abstract PDF (VIETNAMESE)

  The Vietnamese native chickens have low egg production so they is difficult to breeding andtheir population is declining.Polymorphisms (24-bp Insertion-Deletetion và C2402T) of Prolactin geneis usually considered as candidate genes in the genetic analysis for the improved performance traits in poultry. The study was conducted to determine two SNPs polymorphisms (24-bp Insertion-Deletetion and C2402T) of Prolactin gene in two Vietnamese native breeds Ri and Mia chicken.For genotyping of individual was identified by PCR and PCR-RFLP methods. The results showed that the individual’s genotype was identified in two Riand Mia chicken breed. For 24-bp Insertion-Deletetion polymorphism, the II genotype in Ri chicken and Mia chicken present at low frequencies, (0.07) whereas for DD genotypes in Ri and Mia chickens occur at high frequencies, 0.68 and 0.82, respectively. For C2402T polymorphism, the study shown that two breeds (Ri and Mia) had the highest TT genotypes, respectively 0.64 and 0.84; The CC genotype in both Ri and Mia chickens appeared at low frequencies of 0.08 and 0.07, respectively.

• Modeling Cumulative Egg Production Curves of MiaHens using Nonlinear Functions

  Ha Xuan Bo, Hoang Anh Tuan, Pham Kim Dang, Nguyen Hoang Thinh, Bui Huu Doan, Do Duc Luc

  Received: 17-04-2023 / Accepted: 29-08-2023

   Abstract PDF (VIETNAMESE)

  This study was conducted to describe the cumulative egg production curves and determine the best model to estimate the cumulative egg production of Mia hens raised at Hadinco livestock farm, Son Tay Town, Hanoi from June 2021 to May 2022. The cumulative egg production was recorded from 23 weeks (the zero-week egglaying) to 74 weeks of age (51 weeks of the egglaying) from 300 Mia hens. Nine nonlinear models were used to be fitted the data of cumulative egg production of Mia hens. The upper asymptotic cumulative egg production at 52 weeks of egglaying was 85.98 eggs. The number of eggs produced (NEPt) reached the maximum at the 9th week of egglaying (2.57 eggs per hen per week). Lopez function appeared best to describe cumulative egg production of Mia hens with the highest coefficient of determination (99.91%) and the lowest AIC (377.74), BIC (392.99), and SER (0.45). These results suggest that Lopez functions can be applied to estimate the cumulative egg production, number of eggs per hen per week of other local chicken breeds that similar egg production as the Mia hens.

• Modelling Growth Curves to Predict Live Weight, Weight Gains and Suitable Slaughter Age for Mia Chicken

  Hoang Anh Tuan, Ha Xuan Bo, Pham Kim Dang, Nguyen Xuan Trach, Nguyen Hoang Thinh, Bui Huu Doan

  Received: 12-04-2022 / Accepted: 05-07-2022

   Abstract PDF (VIETNAMESE)

  This study was carried out to determine growth curve models for estimating live weight, weight gains and suitable slaughter age for commercial Mia chicken. A total of 300 (150 males and 150 females) birds were raised from 1 to 20 weeks of age and individual live weight data were colletced every week. Three growth functions, viz.Gompertz, Logistic, and Lopez, were used to be fitted with the growth data. The results showed that Gompertz function and Lopez function were the best models for describing the growth curves of the male and the female, respectively. The weekly gain (WGt) reached the maximum at the 10thweek and the 9thweek of age for the male and the female, respectively. The maximum of the average weekly gain (AWGt) was obtained at the 15thweek and the 14thweek of age for the male and the female, respectively.Furthermore, it was estimated that the suitable slaughter time was within 15th-16thweeks of age for the male and 14th-15thweeks for the female of Mia chicken.

• Assessment of Genetic Diversity ofLac Son Chicken using Microsatellite Markers

  Ngo Thi Kim Cuc, Nguyen Van Ba

  Received: 08-04-2019 / Accepted: 22-04-2019

   Abstract PDF (VIETNAMESE)

  The objective of this study was to assess genetic diversity and genetic differentiationbetween Lạc Sơn chicken and other native chicken breeds using 20 microsatellite markers. The resultsindicated that microsatellite markers studied werepolymophic with an average of 6.73 alleles/locus. The genetic diversity of Lạc Sơn chicken was on theaverage level with the expected heterozygosity of 0.60 and the number of alleles/locus was 6.05.The inbreeding coefficient of Lac Son chicken was very low. The lowestgenetic distance was found between Lạc Sơn chicken and Đông Tảo chicken but the highestgenetic distance was found between Lạc Sơn chicken and Tai đỏ chicken.Sub-structuring of the Vietnamese chicken breeds was related to their geographical distributiondistance. Three chicken breeds, viz. Ri, Đong TaoandMia,in the Red River Delta were in the same group, while Lac Son chicken in the northern central region was seperatedand different from Tai Đỏ chicken (Red Jungle Fowl) breed.

• NghiêncứutínhđahìnhExon 14, gen MX ở mộtsốgiốnggàbảnđịacủaViệtNam

  Bui Tran Anh Dao, Dong Thi Hong Lien, Nguyen Thi Phuong Thao, Daniel Desmecht

  Received: 05-05-2013 / Accepted: 25-08-2013

   Abstract PDF (VIETNAMESE)

  Nghiên cứu tính đa hình của exon 14, gen Mx được thực hiện trên 7 giống gà bản địa của Việt Nam (bao gồm: gà Ác, Đông Tảo, H’Mông, Hồ, Mia, Móng và Ri). Kết quả cho thấy tính đa hình của exon 14 xảy ra tại hai vị trí (2032 và 2159). Sự thay thế A hoặc G tại vị trí 2032 của gen Mx có ở tất cả 7 giống gà bản địa được sử dụng trong nghiên cứu này. Trong cùng một giống đều có cả hai alen A và G, tuy nhiên, alen A (liên quan đến tinh kháng virus) có tần số xuất hiện cao hơn so với alen G (liên quan đến tính mẫn cảm với virus). Đặc biệt, tần số của alen A ở giống gà Ri và gà H'mong là cao nhất trong số 7 giống được kiểm tra (với tỉ lệ tương ứng là 80,91% và 74,04%). Phân tích so sánh các axit amin cho thấy sự thay đổi nucleotide A hoặc G tại vị trí 2032 sẽ dẫn đến sự thay đổi axit amin tại vị trí 631 của protein Mx từ Asparagine (liên quan tới khả năng kháng virus của giống) sang Serine (không liên quan tới khả năng kháng virus của giống).