Received: 15-04-2013 / Accepted: 18-06-2013
Thestudy was conducted to establish the procedure for in vitropropagation of Morinda officianalisHow using stem segments. The stem segments were initiate cultured on MS medium containing 0,25 mg/l Kinetinand 1,0 mg/l BA, on which 96,6 % of explants induced shoots after 30 days of culture. The highest shoot multiplication rate (10,13 times) was obtained on MS medium supplemented with 3,0 mg/l BA; 0,2 mg/l IBA and 10,0 mg/l Riboflavin after 45 days of culture. Root induction medium was ½ MS containing 0,2 mg/l IBA and 0,4 g/l activated charcoal.The average rooting frequency was 100 % with a mean of 3.5 roots/shoot within 30 days. In vitroplantlets after 35-days period of rooting were the most appropriateforsuccessfulacclimatization in greenhouse. The highest frequency of acclimatized plantletswas 96,2 %inthe organic substrate containing 50 % of coconut fiber and 50 % of mushroom-production waste materials, the plantlets showed good growth and development.