Vol. 17 No. 8 (2019)

Progesterone is a hormone that relates closely to the reproductive physiology of animals. The progesterone levelsin blood, urine and milk havebeen used to determine pregnancy in cows. In order to detecttheappearance ofprogesterone using specific tests, onemust create hybrid cellsthat secrete monoclonal antibody for this hormone, as one of the most important factors. The methodsof immunization, collection of spleen cellsof BALB/c mice, production of hybrid cell lines and ELISA had been applied. Basedon the use of different types of antigens, mice differently responded to selective progesterone antigens with the concentration of 200 µg/mLper mouse. The highest successful hybridization rate was 91.67%. The study created 5 hybrid cell lines (E4, E3, C6, H3, F10)secreting monoclonal antibodies forprogesterone antigen, 3hybrid cell lines(C12, D7, F11)secreting monoclonal antibodies for progesterone BSAantigenand 3hybrid cell lines(G5, H3, A7)secreting monoclonal antibodies for progesterone-3-CMO: BSA antigen.

The study was conducted to assess the effective use ofArtemiabiomassto replace commercial feedinnursing of mudskipper (Pseudapocryptes elongates). The experimentswereset up comprising 5 treatments with the percentageof Artemiabiomass in dry matter in a feeding ration: 0% (NT1), 25% (NT2), 50% (NT3), 75% (NT4) and 100% (NT5), 3 replicates for each treatment. The natural seeds (3.08 cm; 1.10 g) were obtained from the supplier in Vinh Chau, Soc Trang province. Fishwere nursed in a 200L plastic tank containing 100 L of seawater at 15 ‰, at 100 ind./m2. After a 30-day nursing period, the results showed that the survival of fish was 77.3-86.4%, no significant difference (P >0.05) was observed when compared between the treatments. The length and weight of fish were improved, but there were no significant differences (P>0.05) observed among the treatments except the weight gain of fish fed the NT2 diet was significantly higher than those fed NT1, and weight gain of fish fed NT5 was significantly lower than those of NT1 diet. The results indicated that the commercial feed could be replaced with Artemiabiomass from 25-75% without any adverse effects on the growth and survival rate of mudskipper fingerlings.

Infectious coryza (IC) is an infectious upper respiratory disease affecting chickens and it is caused by Haemophilus paragallinarum (or Avibacterium paragallinarum). The symptoms of IC are facial swelling, malodorous nasal discharge and lacrimation. This study aimed to isolate, identify the Av. paragallinarumof snot in chickens and to determine the sensitivity and resistance to several antibiotics. Ten isolates out of thirteen suspected isolates (76,92%) were Av. paragallinarumby the observation of the morphology of the suspected colony, Gram staining, and biochemical tests (catalase test, oxidase test, urease test, indole test; carbohydrate fermentation such as maltose, lactose, mannitol and sorbitol; motility). The growth of isolates from chickens was not depended on the nicotinamide adenine dinucleotide (NAD). Sensitivity test was done using the ten identified Av. paragallinarumisolates that were cultured on Mueller-Hinton agar and added with antibiotic discs, then incubated in 5% CO2at 37C for 24-48h; results showed that they were 100% sensitive to amoxicillin, ampicillin; 60% sensitive to neomycin, penicillin and 40% sensitive to streptomycin; 100% resistant toward erythromycin, gentamycin, tetracyclin, kanamycin and enrofloxacin.

The experiment was conducted to study the effect of feed protein level onthe growth performance and feed utilisation of ells (Monopterus albus). Four types of industrial feed with protein level were 42, 40, 35 and 30%, respectively, were used in the study, each experiment was repeated thrice. Breeding eels with the average mean body weight of approximately 4 g/eel were distributed in composite tanks of 2 m3, density of 40 eels/m3. The experimental time was 5 months. The protein level of the feed did not affect the eel survival rate, but had a significant effect on the growth rate, feed efficiency and feed cost. Eels using feed with 30% protein content showed low growth rate, low feed efficiency, high feed cost. Eels use feed with 42% protein content, 35% for high growth rate but high cost. Eels using feed with 40% protein content showed a high growth rate, high feed efficiency and lowest feed cost and therefore is recommended for farmers to use.

In order to maintain, protect and develop the gene source of Onychostoma laticeps, the study of biological characteristics of O. laticepswas carried out from January 2018 to December 2018 on 300 samples of O. laticepscollected in the Giang River, Mon Son commune, Con Cuong district, Nghe An province. Results showed that all collected fish samples were in the age of ranging from 1+-3+, corresponding to the weight of 22.5-98.8 g/fish. The O. laticepsis an omnivorous species (Li/Lo was 3.08 ± 0.57). The food composition in the fish intestines was diverse, including more than 28 species, representing for 5 different animal and plant sectors, of which the algae sector was dominated (65%) compared to other sectors. There were no much differences between the Fulton fat coefficient and Clark fat coefficient, this was reflected correctly the level of the nutrient that was accumulated in fish. In nature, O. laticepsstarted breeding in early February, the average absolute fertility was 3,113 eggs, the average relative fertility was 231 eggs/g of the female. The gonosomatic index (GSI) was high during the period from February to June (Male fish ranged from 1.81 to 2.12%; Females fish from 4.62-9.80%) and reached the highest value in March (Male fish: 2.64%; Female fish: 9.80%).

This study aimed to describe the eutrophication status and investigate the main environmental variables driving on the structure of the green algae communities in Tri An reservoir. Samples were monthly collected at 6 stations from March 2016 to February 2017. Eutrophication status was assessed by using the Trophic State Index (TSI). The correlation between green algae communities and environmental parameters was determined by Canonical Correspondence Analysis (CCA). A total of 98 species classified into 31 genera, 16 families, 6 orders, 4 phylawere recorded with a clear dominance of Staurastrumand Scenedesmus. Results of TSI index indicated that water quality in Tri An reservoir was classified as eutrophic to hypertrophic states. The CCA analysis showed that the green algae community was influenced by multivariate factors such as NO3-, PO43-, total P, total N, DO and turbidity. The TSI indexreflected well for eutrophic condition and could be used as a potential application for bio-monitoring of surface water.

Since the PHP server-side programming language was born, it has always been popular with developers for web applications. Until now, PHP has been still the most popular web programming language in the world. The emergence of PHP frameworks has provided web developers with the convenience and efficiency to save time, effort to develop professional web sites based-on built-in features. Our review summarizes the trends of using web programming technology, assesses the advantages and disadvantages of the best six PHP frameworkswhich have beenpointed out by reputable, trusted organizations and individuals. From those, we give suggestions for web developers to choose the framework that best suits their capabilities as well as the requirements of the web application. Finally, the article introduces thetwo web apps as ourcase studies.

The study was conducted to enhance fungi strain and optimize the condition of solid state fermentation for improving the synthesis of multi-enzymes (cellulase, α-amylase and glucoamylase). Aspergillus nigerA45.1 strain was selected for simultaneous mutation treatment by UV and N-methyl-N -nitro-N-nitrosoguanidine (NTG) with mutagenic doses of 0, 30, 60, 90, 120, 150 and 180 minutes to enhancce the secretion of multil-enzymes. After mutation treatments, the Aspergillus sp. GA15 strain with the highest activity of glucoamylase, alpha amylase and celulase enzymes was optimized the fermentation condition to produced multi-enzyme by solid state fermentation. The result found the optimal condition to ferment Aspergillus sp. GA15 was obtained in 5 days fermentation of 2 days old fungi with wheat bran substrate, 1% glucose, 1% urea supplymentation, 50% moisture, pH 5.5 and 30C. Particularly, the activity of glucoamylase, alpha amylase and celulase enzyme was 76,75 U/g; 50 U/g and 40,11 U/g, respectively, which was higher 2,8; 1,29 and 3,3 times compared to normal conditions.

This study aims to determine the conditions for soybean seeds to be able to move on the sowing disc when the centrifugal force created is much smaller than the friction force and the travel time of the seed so that the number of seeds required in the seed collection area is maintained and facilitates for the getting seed of the disc hole. By method of modelling and theoretical analysis of kinematic and dynamic, we have been identified: the angle of the straight directive vaneis fixed to the horizontal position greater than or equal to 70 degrees 50 minutes, the seeds movedalong the directive vaneand the ratio of the time the seed moves through the directive vane length with the disc's rotation time is a constant (k = 0.23), independent on the rotation speed of the sowing disc.