Research on Extraction and Utilization of 2-AP from Pandanus’ Leaf as a Standard for Identification and Quantification of 2-AP in Aromatic Rice

Received: 25-05-2012

Accepted: 05-09-2012

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Issue: Vol. 10 No. 5 (2012)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Hien, P., & Lieu, T. (2024). Research on Extraction and Utilization of 2-AP from Pandanus’ Leaf as a Standard for Identification and Quantification of 2-AP in Aromatic Rice. Vietnam Journal of Agricultural Sciences, 10(5), 747–757. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1697

Research on Extraction and Utilization of 2-AP from Pandanus’ Leaf as a Standard for Identification and Quantification of 2-AP in Aromatic Rice

Phan Phuoc Hien (*) 1 , Truong Thi Bich Lieu 1

  • 1 Trường Đại học Nông Lâm Thành phố Hồ Chí Minh

    • Keywords

    Fragrant rice, SDE-GCFID, SDE-GCMS, thelimit of detection(LOD), the limit of quantification(LOQ), Pandanus amaryllifolius leaves

    Abstract


    So far fragrance of rice has mainly been determined by the traditional sensory method. This evaluation is fast but does not define precisely because it depends entirely on subjective assessment of the evaluator’s olfaction. To overcome this shortcoming, the modern physico-chemical methods, SDE-GCFID and SDE-GCMS were employed. In these analytical processes, quantitative analysis of 2-AP content in pandan’s leaf was performed to serve as basis for comparison with that in aromatic rice. By these methods, since 2005, 2-AP and some other aromatic compounds in aromatic rice have been identified and quantified in thePhysicochemicalLaboratory, Centerfor Biological and Chemical analysis and experiment,University of Agriculture andForestryHo Chi Minh City. In this laboratory from 2010,the content of2-APin theold, matureand young leaves of Pandanus were accurately quantified andused asa standardforquantification of2-AP infragrant rice usingSDE(orSPME)extraction methodcombinedwithGC-FID and GC-MS. The limit of detection(LOD) andlimit of quantification(LOQ) ofthemethod were also determined.

    References

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