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• Production and Characterization of Lignin Peroxidase from White Rot Fungal Strain Tl4

  Trinh Thi Thu Thuy, Nguyen Quoc Trung, Tong Van Hai, Nguyen Hoang Anh

  Received: 29-12-2016 / Accepted: 12-11-2018

   Abstract PDF (VIETNAMESE)

  Lignin is a component of plant cell wall. The degradation of lignin remains a challenge to date due to its stability. Lignin peroxidase (LiP) from white rot fungi was known as a highly effective oxidase enzyme for lignin degradation. In this study, extracellular LiP obtained from strain TL4 was precipitated by (NH4)2SO4and ethanol 96% for obtaining technical enzyme and then characterized for optimal temperature and pH and thermal and pH stability. Results indicated that the optimal temperature and pH of LiP were 30C and 4.0, respectively. This enzyme was stable between 20 and 30C for 2 hours (relative activities remain 94.17% and 89.10%, respectively) and pH from 2.0 to 4.0 for 3 hours (relative activities remain 78.97% and 97.87%), respectively. The LiP from strain TL4 showed potential application in environmental treatment and pre-treatment of cropby-products.

• Study on Identification and Culture Conditionfor Biosynthesis of Laccase by Trametes polyzona TĐ16

  Nguyen Thi Hong Lien, Tran Thi Huong, Nguyen Van Hieu, Dang Thi Nhung, Phan Thi Hong Thao

  Received: 18-08-2023 / Accepted: 26-12-2023

   Abstract PDF (VIETNAMESE)

  White-rot fungi are capble of degrading wood lignin by extracellular lignin modifying enzyme system, in which laccase plays an important role in ligninolysis and is widely used in some industries. This study was carried out with the objective of identifying the white rot fungus TD16 and selected suitable conditions for the biosynthesis of laccase to apply in the biopulping. The strain TD16 was studied for biological characteristics and sequence of the ITS - rDNA gene analyzed. The fruiting bodies have a scalloped shape and the edges are smooth. The upper surface of fruiting bodies is dark brown with concentric circular veins. The hymenium layer is white-brown and rough. On MEA medium, the colonies of TD16 fungal strain are fluffy, snow white, and evenly circular. The strain TD16 has a relatively fast growth rate and can produce extracellular enzymes. The strain TD16 was identified as Trametes polyzonaand named as Trametes polyzonaTD16. The optimal culture conditions for enhanced laccase biosynthesis were investigated. Trametes polyzonaTĐ16 showed the highest laccase activity of upto 8.135 U/ml on MT2 medium containing 0.0625mM CuSO4after 7 days of fermentation at 37ºC and pH of 5.0.