Received: 21-03-2012 / Accepted: 03-06-2012
Research was carried out to produce virus-free plants via meristem culture of Allium wakegi. The explants were apical meristems (0.3 - 0.5mm) derived from virus infected plants. The results indicated that MS medium supplemented with 0.75 mg/l BA and 0.5 mg/l IAA was the most suitable medium for in vitro shoot regeneration from meristem, the rate of regenerated shoots was 73.33%. The highest rate of shoot propagation was 2.0 times with type I shoot and 4.07 times with type II shoot after 4 weeks, on MS medium containing 1.5 mg/l BA. The shoots were transferred to rooting media by addition of 0.5 mg/l IAA and the rate of root formation was 100%, the average number of root was 4.33. RT-PCR method with primers CIfor/ CIRev showed that the regeneration plants from meristem were virus-free.