Received: 06-09-2016 / Accepted: 03-11-2016
An efficient regeneration protocol of large scale micropropagation for clonal establishment ofsweet potato from seedlings is an essential step for clonal selection. The objective of thisstudywas to evaluate the potentials of young coconut water and simple aerated culture conditionin glass bottles with Milliseal®forin vitro propagation of sweet potato using nodal explants.Shoot regeneration of sweet potato nodes bearing dormant buds was effectively promoted by aerated culture condition in full strength MS salt solidified by 8g.L-1agar supplemented with 3% sucrose,BAP 1.0 mg.L-1,0.5 mg.L-1IAA and 20% coconut waterand subsequentroot induction was effective on full strength MS salt solidified by 8g.L-1agar supplemented with 3% sucroseand 0.3 mg.L-1α- NAA. The plantlets derived from aerated culture condition were more vigorous compared to conventional non-aerated culture condition, leading to high survival rate (~ 100%) and enhanced growth during acclimatiztion. Sand -smoked rice husk potting mixwas suitable for hardening of in vitro -derived plantlets.