Isolation and Rapid Identification by MALDI-TOF MS of Riemerella anatipestiferfrom Clinical Cases in Waterfowls in Ha Nam Province

Received: 01-06-2021

Accepted: 10-01-2022

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Issue: Vol. 20 No. 2 (2022)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Lam, T., Lan, N., Huong, N., Anh, D., & Son, T. (2024). Isolation and Rapid Identification by MALDI-TOF MS of Riemerella anatipestiferfrom Clinical Cases in Waterfowls in Ha Nam Province. Vietnam Journal of Agricultural Sciences, 20(2), 192–200. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/951

Isolation and Rapid Identification by MALDI-TOF MS of Riemerella anatipestiferfrom Clinical Cases in Waterfowls in Ha Nam Province

Truong Quang Lam (*) 1, 2 , Nguyen Thi Lan 1 , Nguyen Thi Thu Huong 1 , Dao Le Anh 1 , Tran Danh Son 1

  • 1 Phòng Thí nghiệm trọng điểm Công nghệ sinh học Thú y, Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Isolation, MALDI TOF MS, PCR, Riemerella anatipestifer, waterfowls

    Abstract


    This study aimed to isolate and identify by PCR and MALDI-TOF MS of Riemerella anatipestifer(RA) strains isolated from ducks and muscovy ducks in Ha Nam province. Samples including brain, heart, liver, lung, and spleen were collected from 45 suspected ducks in the farms in Ly Nhan, Binh Luc, Duy Tien districts. Total 27 out of 45 ducks showed the appearance of suspected colonies with the following characteristics: small, transparent, non-hemolysis with the main biochemical features as negative for indole, positive for catalase and oxidase test, and did not grow on MacConkey agar plates. Rapid identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) showed that 27 suspected colonies were positive to RA. This result is 100% in agreement with the PCR results.

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