Application of Direct PCR for Detection of Several Bacteria in Chicken with Respiratory Disease Complex in Hanoi and Neighbour Vicinity

Received: 18-10-2021

Accepted: 10-01-2022

DOI:

Views

0

Downloads

0

Issue: Vol. 20 No. 2 (2022)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Phuong, C., Giap, N., Trung, N., Ngan, M., Ngoc, V., & Le, H. (2024). Application of Direct PCR for Detection of Several Bacteria in Chicken with Respiratory Disease Complex in Hanoi and Neighbour Vicinity. Vietnam Journal of Agricultural Sciences, 20(2), 156–165. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/947

Application of Direct PCR for Detection of Several Bacteria in Chicken with Respiratory Disease Complex in Hanoi and Neighbour Vicinity

Cao Thi Bich Phuong (*) 1 , Nguyen Van Giap 1 , Nguyen Thanh Trung 1 , Mai Thi Ngan 1 , Vu Thi Ngoc 1 , Huynh Thi My Le 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Direct PCR, complex respiratory disease, bacteria, co-infection, poultry, Hanoi

    Abstract


    The respiratory disease complex has emerged as a major economic problem for the poultry industry. In addition, the current diagnostic tools, such as bacteria isolation,serological tests, andconventional PCRare time-consuming and labor-intensive. Therefore, this study was carried out to develop the direct PCR and to apply it to investigate the presence of some common bacteria in the swollen head syndrome. Comparedwith the conventionalPCR results, the Kappavaluefor direct PCR was almost in perfect agreement. Among 173samples screening by direct PCR, 68% of cases revealed infection by atleastoneinvestigatedpathogen, while 23% had concurrent infections by two to three bacteriain a single case. As a result, direct PCR is recommended to be an effective tool for detecting the concurrent infections by Mycoplasma gallisepticum (MG), Avibacterium paragallinarum (APG), Ornithobacterium rhinotracheale (ORT)andavian pathogenicEscherichia coli (APEC)of the swollen head syndrome in chickens in the vicinity of Hanoi.

    References

    Altman D.G. & Bland J.M. (1994). Statistics Notes: Diagnostic tests 1: sensitivity and specificity. BMJ. 308(6943): 1552.

    Aung Y.H., Liman M., Neumann U. & Rautenschlein S. (2008). Reproducibility of swollen sinuses in broilers by experimental infection with avian metapneumovirus subtypes A and B of turkey origin and their comparative pathogenesis, Avian Pathol.37(1): 65-74.

    Alshahni M.M., Makimura K., Yamada T., Satoh K., Ishihara Y., Takatori K. & Sawada T. (2009). Direct colony PCR of several medically important fungi using Ampdirect plus. Jpn J Infect Dis. 62(2): 164-7.

    Andreopoulou M., Franzo G., Tucciarone C.M., Prentza Z., Koutoulis K.C., Cecchinato M. & Chaligianni I. (2019). Molecular epidemiology of infectious bronchitis virus and avian metapneumovirus in Greece, Poult Sci.98(11): 5374-5384.

    Ben-Amar A., Oueslati S. & Mliki A. (2017). Universal directPCR amplification system: a time- and cost-effective tool for high-throughput applications. 3 Biotech. 7(4): 246-246.

    Chen X., Miflin J.K., Zhang P. & Blackall P.J. (1996). Development and application of DNA probes and PCR tests for Haemophilus paragallinarum. Avian Dis. 40(2): 398-407.

    Cascella R., Strafella C., Ragazzo M., Zampatti S., Borgiani P., Gambardella S., Pirazzoli A., Novelli G. & Giardina E. (2015). DirectPCR: a new pharmacogenetic approach for the inexpensive testing of HLA-B*57:01. Pharmacogenomics J. 15(2): 196-200.

    Cao Thị Bích Phượng, Lê Bá Hiệp, Huỳnh Thị Mỹ Lệ & Nguyễn Văn Giáp (2020). Nghiên cứu sự lưu hành của avian metapneumovirus (aMPV) ở gà nuôi tại một số tỉnh miền Bắc Việt Nam, Tạp chí Khoa học Nông nghiệp Việt Nam.18(7):520-528.

    David E. Swayne, Martine Boulianne, Catherine M. Logue, Larry R. McDougald, Venugopal Nair, David L. Suarez, Sjaak de Wit, Tom Grimes, Deirdre Johnson, Michelle Kromm, Teguh Yodiantara Prajitno, Ian Rubinoff & Guillermo Zavala (2020). Diseases of poultry (14th). John Wiley & Sons, Inc., Hoboken, NJ 07030, USA.

    De Boeck C., Kalmar I., Dumont A. & Vanrompay D. (2015). Longitudinal monitoring for respiratory pathogens in broiler chickens reveals co-infection of Chlamydia psittaci and Ornithobacterium rhinotracheale. J Med Microbiol. 64(Pt 5): 565-574.

    De Oliveira A.L., Rocha D.A., Finkler F., De Moraes L.B., Barbieri N.L., Pavanelo D.B., Winkler C., Grassotti T.T., De Brito K.C., De Brito B.G. & Horn F. (2015). Prevalence of ColV plasmid-linked genes and in vivopathogenicity of avian strains of Escherichia coli, Foodborne Pathog Dis. 12(8): 679-85.

    Đào Thị Hảo (2008). Phân lập, xác định một số đặc tính sinh học của Mycoplasma gallisepticum& chế kháng nguyên, huyết thanh chẩn đoán. Luận án Tiến sĩ Nông nghiệp. Viện Thú y.

    Eszik I., Lantos I., Önder K., Somogyvári F., Burián K., Endrész V. & Virok D.P. (2016). High dynamic range detection of Chlamydia trachomatis growth by direct quantitative PCR of the infected cells. J Microbiol Methods. 120: 15-22.

    Feuerman M. & Miller A.R. (2008). Relationships between statistical measures of agreement: sensitivity, specificity and kappa. J Eval Clin Pract. 14(5): 930-3.

    Goodwin M.A. & Waltman W.D. (1994). Clinical and pathological findings in young Georgia broiler chickens with oculofacial respiratory disease ("so-called swollen heads"). Avian Dis. 38(2): 376-8.

    García M., Ikuta N., Levisohn S. & Kleven S.H. (2005). Evaluation and comparison of various PCR methods for detection of Mycoplasma gallisepticum infection in chickens. Avian Diseases.49(1): 125-132.

    Gharaibeh S.M. & Algharaibeh G.R. (2007). Serological and molecular detection of avian pneumovirus in chickens with respiratory disease in Jordan. Poult Sci. 86(8): 1677-81.

    Johnson T.J., Wannemuehler Y., Doetkott C., Johnson S.J., Rosenberger S.C. & Nolan L.K. (2008). Identification of minimal predictors of avian pathogenic Escherichia colivirulence for use as a rapid diagnostic tool. J Clin Microbiol. 46(12): 3987-96.

    Jones R. & S.R. (2013). Diseases of poultry. In:Avian metapneumovirus: David E. Swayne, J.R.G., Larry R. Mcdougald, Lisa K. Nolan, David L. Suarez, Venugopal L. Nair (ed.)13 ed. John Wiley & Sons Inc. Iowa, USA: Iowa State Press.

    Katcher H.L. & Schwartz I. (1994). A distinctive property of Tth DNA polymerase: enzymatic amplification in the presence of phenol. Biotechniques. 16(1): 84-92.

    Kwon J.S., Lee H.J., Jeong S.H., Park J.Y., Hong Y.H., Lee Y.J., Youn H.S., Lee D.W., Do S.H., Park S.Y., Choi I.S., Lee J.B. & Song C.S. (2010). Isolation and characterization of avian metapneumovirus from chickens in Korea. J Vet Sci. 11(1): 59-66.

    Kaore M., Singh K., Palanivelu M., Asok Kumar M., Reddy M. & Kurkure N.V. (2018). Patho-epidemiology of respiratory disease complex pathogens (RDPs) in commercial chicken. Indian J. Vet. Pathol. 42(4): 231-238.

    Lorenz T.C. (2012). Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. Journal of visualized experiments: JoVE. (63): e3998-e3998.

    Lê Văn Hùng, Nguyễn Thị Giang & Trần Danh Sơn (2019). Phân lập, xác định đặc điểm của vi khuẩn gây bệnh sổ mũi truyền nhiễm trên gà tại một số tỉnh phía Bắc Việt Nam. Tạp chí Khoa học Nông nghiệp Việt Nam.17(8): 622-629.

    Mcdougall J.S. & Cook J.K. (1986). Turkey rhinotracheitis: preliminary investigations. Vet Rec. 118(8): 206-7.

    Mohamed M. Shawki Lebdah M.A., Shahin A.M. & Nassif S. A. (2017). Some studies on swollen head syndrome in broiler chickens in Egypt, Zagazig Veterinary Journal. 45(S1): 132-141.

    Nakamura K., Mase M., Tanimura N., Yamaguchi S. & Yuasa N. (1998). Attempts to reproduce swollen head syndrome in specific pathogen-free chickens by inoculating with Escherichia coliand/or turkey rhinotracheitis virus. Avian Pathol. 27(1): 21-7.

    Nguyễn Thị Lan, Chu Đức Thắng, Nguyễn Bá Hiên, Phạm Hồng Ngân, Lê Văn Hùng & Nguyễn Thị Yến (2016). Đặc điểm của vi khuẩn Ornithobacterium rhinotracheale(ORT) phân lập từ đàn gà nuôi tại một số tỉnh phía Bắc Việt Nam. Tạp chí Khoa học Nông nghiệp Việt Nam. 14(11): 1734-1740.

    Nguyễn Thị Loan, Lê Đình Quyền, Dương Hồng Quân, Lê Huỳnh Thanh Phương, Nguyễn Bá Hiên & LêVăn Phan. (2016). Ứng dụng kỹ thuật RT-PCR để chẩn đoán bệnh viêm phế quản truyền nhiễm (Infectious bronchitis) ở gà đẻ trứng tại một số tỉnh phía Bắc Việt Nam. Tạp chí Khoa học Nông nghiệp Việt Nam. 14(9): 1387-1394.

    Paudel S., Hess M. & Hess C. (2017). Coinfection of Avibacterium paragallinarum and Gallibacterium anatis in Specific-Pathogen-Free Chickens Complicates Clinical Signs of Infectious Coryza, Which Can Be Prevented by Vaccination. Avian Dis. 61(1): 55-63.

    Roussan D.A., Haddad R. & Khawaldeh G. (2008). Molecular survey of avian respiratory pathogens in commercial broiler chicken flocks with respiratory diseases in Jordan. Poult Sci. 87(3): 444-8.

    Samy A. & Naguib M.M. (2018). Avian respiratory coinfection and impact on avian influenza pathogenicity in domestic poultry: field and experimental findings. Vet Sci. 5(1): 23.

    Tanaka M., Takuma H., Kokumai N., Oishi E., Obi T., Hiramatsu K. & Shimizu Y. (1995). Turkey Rhinotracheitis Virus Isolated from Broiler Chicken with Swollen Head Syndrome in Japan. Journal of Veterinary Medical Science. 57(5): 939-941.

    Trương Hà Thái, Nguyễn Ngọc Đức, Nguyễn Văn Giáp & Chu Thị Thanh Hương (2009). Xác định tỉ lệ nhiễm Mycoplasma gallisepticum ở 2 giống gà hướng thịt Ross 308 & ISA màu nuôi công nghiệp tại một số tỉnh miền Bắc Việt Nam. Tạp chí Khoa học & Phát triển. 7(3): 306-313.

    Van Empel P.C. & Hafez H.M. (1999). Ornithobacterium rhinotracheale: A review. Avian Pathol. 28(3): 217-27.

    Võ Thị Trà An, Nguyễn Thị Bích Liên, Trần Thị Ngọc Hân, Hồ Quang Dũng & Chansiripornchai, N. (2014). Nhận dạng, phân lập & xác định mước độ mẫn cảm kháng sinh của vi khuẩn Orninobacterium rhinotrachealeở gà. Tạp chí Khoa học Kỹ thuật Thú y. 21(7): 23-27.

    Wilfinger W.W., Mackey K. & Chomczynski P. (1997). Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques. 22(3): 474-6, 478-81.

    Yu M., Xing L., Chang F., Bao Y., Wang S., He X., Wang J., Wang S., Liu Y., Farooque M., Pan Q., Wang Y., Gao L., Qi X., Hussain A., Li K., Liu C., Zhang Y., Cui H., Wang X. & Gao Y. (2019). Genomic sequence and pathogenicity of the first avian metapneumovirus subtype B isolated from chicken in China. Vet Microbiol. 228: 32-38.

    Zhong C., Gopinath S., Norona W., Ge J., Lagacé R.E., Wang D.Y., Short M.L. & Mulero J.J. (2019). Developmental validation of the Huaxia™ Platinum PCR amplification kit: A 6-dye multiplex direct amplification assay designed for Chinese reference samples. Forensic Science International: Genetics. 42: 190-197.