Received: 10-06-2020
Accepted: 21-01-2021
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Influence of Single Nucleotide Polymorphism G/T at 5’-Splice Site of Intron 1 of WaxyGene on Amylose Content and Gel Consistency in Vietnamese Rice
,
Luu Thi Van
2
,
Nguyen Chinh Viet
3
,
Tong Van Hai
1
Keywords
Waxygene, amylose content, gel consistency, CAPS marker, single nucleotide polymorphism
Abstract
The cooking and eating quality of rice, which are defined by amylose content (AC) and gel consistency (GC), are important criteria determiningthe export price of Vietnamese rice. These properties mainly depend on the amylose content in rice grain endosperm and are regulated by the Waxygene. This study aimedto determine the effect of point mutation G/T in the first intron of Waxygene with AC and GT traits in Vietnamese rice accessions. The AC evaluation in 64 accessions resultedfrom26 accessions with high AC and 5 accessions with average AC; 20 accessions with low AC; 2 accessions had very low AC and 11 accessions of glutinous rice, respectively. The GC evaluation results classified the materials into 5 groupsincluding: very hard (25 accessions); hard (1 accession); medium (13 accessions); soft (11 accessions) and very soft (14 accessions). Using CAPS markers, there were 31 samples possessed SNP G and Wxaalleles and 33 samples possessed SNP T and Wxballeles. Comparison of genotypic and phenotypic results showed that the PCR-AccI has high accuracy for detection of Waxygenotype and needed to apply in breeding programs for quality rice.
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