Received: 25-05-2020
Accepted: 12-09-2020
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Effects of adding chicken egg yolk and caffeine in extender on the quality of goat semen
,
Tran Thi Chi
1
,
Vu Hai Yen
1
,
Nguyen Tuan Dung
1
,
Trinh Thi Linh Chi
1
,
Su Thanh Long
1
Keywords
Goat semen, semen extender, egg yolk, caffeine
Abstract
The aims of this study were to assess the effects of adding chicken egg yolk and caffeine in TCG (Tris-Citrate-Glucose) extender on the quality of goat semen preserved some goat breeds in Vietnam. Semen samples of 9 bucks belonging to 3 goat breeds, including Saanen, Boer and Alpine, (3 bucks/breed) were collected using artificial vagina, then diluted at a ratio of 1:10 with TCG extender added 0%, 5%, 10%, 15%, and 20% chicken egg yolk and 0,02; 0,03 and 0,04g caffeine/l. The diluted semen samples were preserved at 5 and 15C, respectively. The sperm motility and sperm morphological parameters of the preserved semen samples were assessed and compared after 24h, 72h, and 120h of preservation. Results showed that addition of 0,03g caffeine/l in TCG extender gave a better quality of preserved goat semen at both temperatures. At 5C, TCG extender added 10% of chicken egg yolk gave a better quality of preserved goat semen and could be used for artificial insemination after 72h of preservation. At 15C, TCG extender added 15% of chicken egg yolk resulted in a better quality of preserved goat semen and could be used for artificial insemination after 24h of preservation.
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