Virulence in Experimental Pigs of Field African Swine Fever Virus Strain(VNUA - ASFV - L01)Isolated from Infected Pig in Ha Nam Province of Vietnam

Received: 18-05-2020

Accepted: 23-06-2020

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Issue: Vol. 18 No. 7 (2020)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Lam, T., Lan, N., Anh, D., Hoa, N., & Huong, N. (2024). Virulence in Experimental Pigs of Field African Swine Fever Virus Strain(VNUA - ASFV - L01)Isolated from Infected Pig in Ha Nam Province of Vietnam. Vietnam Journal of Agricultural Sciences, 18(7), 510–519. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/687

Virulence in Experimental Pigs of Field African Swine Fever Virus Strain(VNUA - ASFV - L01)Isolated from Infected Pig in Ha Nam Province of Vietnam

Truong Quang Lam (*) 1, 2 , Nguyen Thi Lan 3 , Dao Le Anh 3 , Nguyen Thi Hoa 3 , Nguyen Thi Thu Huong 3

  • 1 Phòng Thí nghiệm trọng điểm Công nghệ sinh học Thú y, Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 3 Phòngthí nghiệm trọng điểm CNSH Thú y, KhoaThú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    African swine fever virus (ASFV), Virulence, clinical signs, pathological lesions.Viremia, shedding

    Abstract


    This study aimedto assess the virulence in experimental pigs of field African swine fever virus strain (VNUA - ASFV -L01) belonged to genotype 2 which isolated from the infected pig duringan outbreakoccurredin Ha Nam province of Vietnam, 2019. The experimental pigs were intramuscularly inoculated with the virus at a dose of 104 HAD50. All inoculated pigs showed acute disease manifestations and died between 7and 9 days post-infection (dpi) with clinical features of ASF including high fever (41-42°C), diarrhea, vomiting, loss of appetite and reflexes, depression, respiratory distress, haemorrhages in the skin, neurological signs, coma and death. Haemorrhagic lesions were typically observed in multiple tissue organ in ASFV infected pigs with remarkable signs of diffuse hemorrhages in lymph nodes, renal cortex, haemorrhagic spleen, swollen liver, edema of the gallbladder, and hemorrhagic meninges... Analysis of viremia inthe blood of pigs by real-time PCR showedthat ASFV viremia was detected in the blood of pigs at 2 days post-inoculation, thereafter rapidly replication of field virus strain was observed in the blood of infected pigs at a later time point post-infection (days 3 to 6). These initial results indicate that VNUA - ASFV -L01 strain is highly virulent in pigs.

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    https://www.oie.int/fileadmin/Home/enq/Health_standards/tahrn/3.08.01_ASF.pdf