Establishing Virus Neutralizing TestPorcine Epidemic Diarrhea Virus (PEDV)using Isolated Field Strains in the North of Viet Nam

Received: 18-05-2020

Accepted: 23-06-2020

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Issue: Vol. 18 No. 7 (2020)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Bich, N., Khanh, T., Ba, N., & Huong, C. (2024). Establishing Virus Neutralizing TestPorcine Epidemic Diarrhea Virus (PEDV)using Isolated Field Strains in the North of Viet Nam. Vietnam Journal of Agricultural Sciences, 18(7), 475–484. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/683

Establishing Virus Neutralizing TestPorcine Epidemic Diarrhea Virus (PEDV)using Isolated Field Strains in the North of Viet Nam

Nguyen Thi Bich (*) 1 , Tran Van Khanh 1 , Nguyen Thanh Ba 1 , Chu Thi Thanh Huong 2

  • 1 Công ty TNHH Dược Hanvet
  • 2 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Porcine epidemic diarrhea virus, virus neutralizing test (VNT)

    Abstract


    The study aimed to establish a virus neutralization test for field isolated virus, create a tool for assessing the protection of vaccines against pathogenic porcine epidemic diarrhea virus (PEDV) strains in Vietnam. By using DMEM medium free calf fetal serum (FBS) and without trypsin for dilution sample preparation/viral suspensions, and the maintenance medium containing 8 µg of trypsin/ml, the virus neutralization test and evaluation of neutralizing antibodies by immunochemical staining on monolayer cells were optimized. The results showed that the virus neutralization test for field isolated virus with the sensitivity of 87.46% and specificity 98.18% with compared to ELISA kit. It also pointed out that the virus neutralization test could be applicated to detect fluctuation of PEDV neutralization antibody in colostrum and serum of mother and piglets.

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