Received: 14-08-2018
Accepted: 18-03-2019
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Effect of Light Emiting Diode Lighting and Elicitors on Biomass and Antioxidant Activity of Solanum hainaneseCalluses
,
Nguyen Thi Thanh Phuong
3
,
Doan Ngoc Han
4
,
Nguyen Van Trinh
5
Keywords
Solanum hainanense, callus culture, LEDlighing, salicylic acid, yeast extract
Abstract
Solanum hainanense,a valuablemedicinal herbis used for detoxifying and protecting the liver. This plant accumulates different alkaloids and glycol-alkaloids that could inactivate Hepatitis B virus, acting as antioxidant and preventing cirrhosis effectively. Callus culture is a potential method to producesafe and high quality medical materials. Light spectrum and elicitors (yeast extract and salicylic acid) hadsignificantly positive effect on the increase of biomass and antioxidant activity of Solanum hainanensecallus culture on gel medium. LED lighting with a 30/70% of Blue/Red spectrum increased the dry biomass of callusesby 2.46 times compared with lighting by T8 fluorescence lamps. When adding 100 M salicylic acid or 3g/L yeast extract to the culture medium, the dry biomass of the calluseswas 2.43 or 3.04 times higher than the control, respectively. In addition, elicitors increased the antioxidant activity of the callus extract from 1.26 to 1.49 times of that of the control callus extract and increased from 2.31 to 2.73 times compared tothe two-year-old natural herbalextracts. These results could serve as the basisfor the development of in vitroculture systemfor improving the biomass and antioxidant activity of Solanum hainanense callusesand for expanding the research to other medicinal plants.
References
Nguyễn Hữu Thuần Anh (2016). Nghiên cứu ảnh hưởng của một số elicitor lên khả năng tích lũy solasodine ở tế bào in vitrocủa cây cà gai leo (Solanum hainanense Hance). Luận án tiến sĩ Sinh lý học thực vật. Trường đại học Khoa học, Đại học Huế.
Cioć Monika, Agnieszka Szewczyk, Marek Żupnik, Andrzej Kalisz, Bożena Pawłowska (2018). LED lighting afects plant growth, morphogenesis and phytochemical contents of Myrtus communis L. in vitro.Plant Cell Tiss Organ Cult.,132: 433-447.
Gamborg O.L., Philip G.C. (1995). Plant cell, tissue and organ culture. Pub. Springer, pp. 37-39.
Gupta S.D., Jatothu B. (2013). Fundamentals and applications of light-emitting diodes (LEDs) in in vitroplant growth and morphogenesis. Plant Biotechnol Rep., 7:211-220.
Hasan Md. Mohidul,Tufail Bashir,Ritesh Ghosh,Sun Keun Lee, Hanhong Bae(2017). An Overview of LEDs’ Effects on the Production of Bioactive Compounds and Crop Quality. Molecules, 22(9): 1420; https://doi.org/10.3390/molecules22091420.
Hussain Sarfaraj Md., Sheeba Fareed, Saba Ansari, Md. Akhlaquer Rahman, Iffat Zareen Ahmad, Mohd. Saeed(2012). Current approaches toward production of secondary plant metabolites. J Pharm Bioallied Sc.,4(1): 10-20.
Nguyễn Thị Minh Khai, Phạm Kim Mãn, Nguyễn Bích Thu, Vũ Kim Thu, Phạm Thanh Trúc, Lã Kim Oanh, Nguyễn Văn Mùi, Trịnh Thị Xuân Hòa, Nguyễn Anh Tuấn, Mão NĐ(?)(2001). Nghiên cứu điều chế thuốc Haina điều trị viêm gan B mạn hoạt động từ cà gai leo. Tạp chí Dược liệu, 6(2): 68-71.
Loc N., Giang N. (2012). Effects of elicitors on the enhancement of asiaticoside biosynthesis in cell cultures of centella (Centella asiaticaL. Urban). Chemical Papers,66(7): 642-648.
Nguyen Hoang Loc, Nguyen Huu Thuan Anh, Le Thi Minh Khuyen, Ton Nu Thuy An (2014). Effects of yeast extract and methyl jasmonate on the enhancement of solasodine biosynthesis in cell cultures of Solanum hainanense Hance.J. BioSci. Biotech.,3(1): 1-6.
Murashige T. and Skoog F. (1962). A revised medium for rapid growth bio assys with tobacco tissue cultures. Physiology Plant,15: 473-479.
Nhut D.T., Nguyen P.L.H., Don N.T., Hien N.T.T., Huy N.P., Nam N.B., Vinh B.T., Luan T.C. (2014). Induction, growth and paclitaxel content of needle and petiole-derived calli in Hymalayan Yew (Taxus wallichianaZucc.) under light-emitting diodes. Acta Biologica Cracoviensia Series Botanica 56/2:107-114.
Nhut D.T., N.P. Huy, N.T. Tai, N.B. Nam, V.Q. Luan, V.T. Hien, H.T. Tung, B.T. Vinh, T.C. Luan (2015). Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensisHa et Grushv. Biotechnology and Biotechnological Equipment 29(2):299-308.
SharmaM., SharmaA., Kumar A., Kumar S.B. (2011). Enhancement of Secondary Metabolites in Cultured Plant Cells Through Stress Stimulus. American Journal of Plant Physiology,6(2): 50-71.
Stroev E. A., Makarova V. G., (1989). Determination of lipid peroxidation rate in tissue homogenate laboratory. In: Laboratory Manual in Biochemistry, Mir Publishers, Moscow, pp. 243 -256.
WangJuan,Jian-liLi,JingLi,Jin-xinLi,Shu-jieLiu,Lu-qiHuang,Wen-yuan Gao(2017).Production of Active Compounds in Medicinal Plants: From Plant Tissue Culture to Biosynthesis. Chinese Herbal Medicines, 9(2): 115-125
Yu L.J., Lan W.Z., Qin W.M., Xu H.B. (2001). Effects of salicylic acid on fungal elicitor-induced membrane-lipid peroxidation and taxol production in cell suspension cultures of Taxus chinensis.Process Biochemistry,37(5): 477-482.