Study on Some Biological and Molecular Biological Characteristicsof the KTY-PRRS-01 Virus Strain Isolated in Vietnam

Received: 27-02-2017

Accepted: 25-04-2017

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Issue: Vol. 15 No. 2 (2017)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Son, P., Lan, N., Cam, N., & Hien, N. (2024). Study on Some Biological and Molecular Biological Characteristicsof the KTY-PRRS-01 Virus Strain Isolated in Vietnam. Vietnam Journal of Agricultural Sciences, 15(2), 188–197. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/351

Study on Some Biological and Molecular Biological Characteristicsof the KTY-PRRS-01 Virus Strain Isolated in Vietnam

Pham Van Son (*) 1 , Nguyen Thi Lan 2 , Nguyen Van Cam 3 , Nguyen Ba Hien 2

  • 1 Nghiên cứu sinh, Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 2 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 3 Hội Thú y Việt Nam

    • Keywords

    PRRS, stablebiological and molecular biological characteristics

    Abstract


    The study was carried out on the KTY-PRRS-01 virus strain isolated in Vietnam. The PRRSV strains were cultured for 5thconsecutive generations on the Marc145 cell and in each passage the virus was tested for biology and molecular biology characteristics. The results of the study showed that through 5th passages, the ability to multiply and destroy the cells of the original virus over time had no significant difference in the generations. The virus began to multiply and had cytopathogenic effect after 48 hours post - infection, cytopathogenic effect reached 80% at 72 hours post - infection, and the cells were completely destroyed after 84 hours post - infection. TCID50 of virus was stable through 5th passages with a titre from 3,03x106/ml to 7,12x106/ml. The ORF5 and ORF7 gene sequences of the virus strain through 5th passages were sequenced. Sequences of these genes showed no difference in nucleotide sequences as well as amino acids through 5th passages. Thus the KTY-PRRS-01 virus strain is stable in biological and molecular biology characteristics over 5th passages.

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