Meristem Culture ofAllium wakegi

Received: 21-03-2012

Accepted: 03-06-2012

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Issue: Vol. 10 No. 3 (2012)

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NÔNG HỌC

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Trang, P., Anh, N., & Thanh, H. (2024). Meristem Culture ofAllium wakegi. Vietnam Journal of Agricultural Sciences, 10(3), 402–410. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/20

Meristem Culture ofAllium wakegi

Pham Thi Thu Trang (*) 1 , Nguyen Thi Ly Anh 1 , Ho Thi Thu Thanh 1

  • 1 Viện Sinh học Nông nghiệp, Trường Đại học Nông nghiệp Hà Nội

    • Keywords

    Allium, Allium wakegi, De onion, Meristem culture

    Abstract


    Research was carried out to produce virus-free plants via meristem culture of Allium wakegi. The explants were apical meristems (0.3 - 0.5mm) derived from virus infected plants. The results indicated that MS medium supplemented with 0.75 mg/l BA and 0.5 mg/l IAA was the most suitable medium for in vitro shoot regeneration from meristem, the rate of regenerated shoots was 73.33%. The highest rate of shoot propagation was 2.0 times with type I shoot and 4.07 times with type II shoot after 4 weeks, on MS medium containing 1.5 mg/l BA. The shoots were transferred to rooting media by addition of 0.5 mg/l IAA and the rate of root formation was 100%, the average number of root was 4.33. RT-PCR method with primers CIfor/ CIRev showed that the regeneration plants from meristem were virus-free.

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