Selection of Porcine Circovirus Type 2(PCV2) Isolates for Development ofVaccine against Postweaning Multisystemic Wasting Syndrome

Received: 28-01-2015

Accepted: 18-04-2015

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Issue: Vol. 13 No. 3 (2015)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Le, H., Phan, L., Giap, N., & Thau, T. (2024). Selection of Porcine Circovirus Type 2(PCV2) Isolates for Development ofVaccine against Postweaning Multisystemic Wasting Syndrome. Vietnam Journal of Agricultural Sciences, 13(3), 406–415. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/178

Selection of Porcine Circovirus Type 2(PCV2) Isolates for Development ofVaccine against Postweaning Multisystemic Wasting Syndrome

Huynh Thi My Le (*) 1 , Le Van Phan 1 , Nguyen Van Giap 1 , Trinh Dinh Thau 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Porcine circovirus type 2, master seed, vaccine

    Abstract


    This study aimed to build up a collection of field isolates ofPorcine circovirus type 2 (PCV2) circulating in pigs of Viet Nam.This is the first study for development of vaccine against post-weaning multi-systemic wasting syndrome, one of the most important Porcine circovirus type 2-associated diseases. Seven isolates of PCV2 were selected as candidates for subsequent evaluations. The purity test showed that 100% of PCV2 isolates were free of CSFV, PRRSV, PPV, PEDV, TGEV, PRV, PAdV and Mycoplasma. However, there were two out of sevenisolates were contaminated with PCV1, and thus were not eligible for the production of master seed. The titers of fivepurePCV2 isolates were in the rangefrom 102.00TCID50/ 0.1 ml to 105.75TCID50/ 0.1 ml, of which the titers of threeisolates werehigher than 105.00TCID50/ 0.1 ml.

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