Received: 18-04-2017
Accepted: 26-07-2017
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In VitroAnti-Bacterial Activity of Herbal Extracts against Staphylococcusspp. and Streptococcus spp. Isolated from the Metritis in Cows
,
Nguyen Thanh Hai
2
,
Nguyen Nam Phuong
3
,
Nguyen Van Thanh
1, 2
Keywords
Extract of herbs, Anti - bacterial effect, Staphylococcusspp., Streptococcusspp., cows metritis
Abstract
The use of antibioticsfor the cow metritis treatment has many advantages, however, raises concerns about antibiotic residues in the meat and dairy products as well as increasing antibiotic resistance of pathogenic bacteria. Herbs have been increasingly demonstrated their significant role in the pharmaceutical industry as a biosafety solutionandan alternative to synthetic chemical drugs. The present study aimed to examine the anti-bacterial activity of herbal extracts(Polyalthia longifolia var.Pendula, Lignum Caesalpiniae sappari, Excoecaria cochinchinensis Lour.,Clerodendron fragrans Vent, Wedelia calendulaceae Less, Cudrania tricuspidata, Pseuderanthemum (Ness)Radik, Lactuca indica L)against Staphylococcusspp. and Streptococcusspp.isolated from cow metritis. The resultsshowed that the extractionefficiency varied from 9.60% (Lignum Caesalpiniae sappari) to 14.32% (Wedelia calendulaceae Less). At the concentration of 100 mg/ml, all the extracts showed good anti-bacterial activity against Staphylococcusspp. and Streptococcusspp. For Streptococcusspp., the bacterial inhibition zone varied from 11.80 mm (Cudrania tricuspidata(Carr) to 25.00 mm (Clerodendron fragrans Vent). The diameter of the bacterial inhibition zone of Staphylococcusspp. varied from 10.28 mm (Cudrania tricuspidata(Carr)) to 25.30 mm (Wedelia calendulaceae Less). The extract solution of Clerodendron fragransVent and Wedelia calendulaceaeLess showed the highest anti-bacterial activity. This extract remained its anti-bacterial activity against Staphylococcus spp. and Streptococcusspp. at concentration of 0.20mg/ml.
References
Ahmed F., Saxena M., Maini S. (2014). Aherbal intrauterine infusion “Arasksha liquid” for treatment of reproductive disorders in cows. IJPRBS, 3(2): 42 - 48.
AmadouC. K. (1998). Promoting Alternative Medicine. Africa Health Journal, 2: 20 - 25.
Cui D., Li J., Wang X., Xie J., Zhang K., Wang X., Zhang J., Wang L., Qin Z., and Yang Z. (2014). Efficacy of herbal tincture as treatment option for retained placenta in dairy cows. Anim Reprod Sci., 145: 23 - 28.
Dubuc J., Duffield T. F., Leslie K. E., Walton J. S., and LeBlanc S. J. (2011). Effects of postpartum uterine diseases on milk production and culling in dairy cows. Journal of Dairy Science, 94: 1339 - 1346.
Duricic D. M., Lipar M., Samardžija M. (2014). Ozone treatment of metritis and Ozone treatment of metritis and endometritis in Holstein cows endometritis in Holstein cows. Vet. Arhiv.,84: 103 - 110.
Gilbert R. O., Shin S. T., Guard C. L., Erb H. N., and Frajblat M. (2005). Prevalence of endometritis and its effects on reproductive performance of dairy cows. Theriogenology, 64: 1879 - 1888.
Leelapornpisid P., Chansakao S., Ittiwittayawat T., Pruksakorn S. (2011). Antimicrobial activity of herbal extracts on Staphylococcus Aureus and Propionibacterium Acnes. ISHS Acta Horticulturae 679: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 5: Quality, Efficasy, Sefety, Processing and Trade in Medicinal and Aromatic Plants.
Mahesh B., Satish S. (2008). Antimicrobial activity of some important medicinal plants against animal and human pathogens. World J Agric Sci., 4(S): 839 - 843.
Marquez A., Gonzalez M., Bonges H. (2007). Effects of intrauterine administration Montanoa tomentosa ectracts upon postpartum metritis in dairy cows. AJOL, 3(1).
Meziane R., Abdellatif Niar, Mustapha Adnane Smadi, Bakir Mamache (2013). A clinical study of metritis in dairy cows in the region of Batna (east of Algeria) and their treatments using different therapeutic protocols. Veterinary World, Vet World, 6(1): 45 - 48.
Nguyễn Thanh Hải, Bùi Thị Tho (2013). Nghiên cứu tác dụng diệt khuẩn in vitro của dịch chiết tỏi (Allium sativum L. )đối với vi khuẩn E. coli gây bệnh và E. coli kháng Ampicillin, Kanamycin. Tạp chí Khoa học và Phát triển, 11(6): 804 - 808.
Nguyễn Thanh Hải, Bùi Thị Tho, Miyamoto Atsushi (2014). Nghiên cứu ảnh hưởng của dung môi đến hiệu suất chiết và tác dụng diệt khuẩn in vitro của lá cây huyền diệp đối với E. coli và Salmonella phân lập từ phân vịt tiêu chảy. Tạp chí Khoahọc Kỹ thuật Thú y, XXI(3): 74 - 81.
Nguyễn Thanh Hải, Nguyễn Văn Thanh (2016). Nghiên cứu tác dụng ức chế in vitro của cao khô dịch chiết dược liệu trên vi khuẩn Staphylococcus aureus, Streptococcus spp và E. coli phân lập từ dịch viêm tử cung chó và thử nghiệm điều trị. Tạp chí Khoahọc Kỹ thuật Thú y, XXIII(4): 26 - 36.
Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur (2011). Phytochemical screening and Extraction: A Review. Internationale pharmaceutica sciencia, 1(1): 98 - 106.
Sarkar H., Kumar M., Rawat V. P., Varshney T. K., Goswami M. C., Yadav and Srivastava S. K. (2006). Effect of Administration of Garlic Extract and PGF2α on Hormonal Changes and Recovery in Endometritis Cows. Asian - Aust. J. Anim. Sci., 19(7): 964 - 969.
World Health Organization (WHO) (2014). Antimicrobial resistance: Global report on surveillance. WHO Library Cataloguing - in - Publication Data.