Evaluation of the Pathogenicity of Streptococcus AgalactiaeSerotype Ia and III in Tilapia under Experimental Challenges

Received: 20-06-2023

Accepted: 05-10-2023

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Issue: Vol. 21 No. 10 (2023)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Nhinh, D., Son, N., Hoa, D., Hang, N., Thoa, N., Giang, N., … Hoai, T. (2024). Evaluation of the Pathogenicity of Streptococcus AgalactiaeSerotype Ia and III in Tilapia under Experimental Challenges. Vietnam Journal of Agricultural Sciences, 21(10), 1256–1269. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1196

Evaluation of the Pathogenicity of Streptococcus AgalactiaeSerotype Ia and III in Tilapia under Experimental Challenges

Doan Thi Nhinh (*) 1, 2 , Nguyen Vu Son 3 , Dang Thi Hoa 2 , Nguyen Thi Thuy Hang 4 , Ngo Phu Thoa 2 , Nguyen Thi Huong Giang 3 , Kim Van Van 2 , Dang Thi Lua 1 , Truong Dinh Hoai 5, 6, 2

  • 1 Viện Nghiên cứu Nuôi trồng Thủy sản I
  • 2 Khoa Thủy sản, Học viện Nông nghiệp Việt Nam
  • 3 Khoa Thú y, Học viện Nông nghiệp Việt Nam
  • 4 Trường Cán bộ Quản lý Nông nghiệp và Phát triển nông thôn
  • 5 Khoa Chăn nuôi và NTTS
  • 6 Faculty of Animal Science and Aquaculture, Vietnam National University of Agriculture

    • Keywords

    Streptococcus agalactiae, serotype, pathogenicity, tilapia, Vietnam

    Abstract


    The study was conducted to evaluate the pathogenicity of Streptococcus agalactiaeserotypes Ia and III causing disease in tilapia. A total of 10 isolates of suspected S. agalactiaeretrieved from diseased tilapia in northern Vietnam in 2022 were used for identification by biochemical method and by PCR assays and serotyping by multiplex PCR. The results revealed that of 10 identified isolates of S. agalactiaeseven isolates belongedto serotype III (ST III) and three others belonged to serotype Ia (ST Ia). The experimental challenges on tilapia of the representative of each serotype showed that fish infected with the ST III isolates caused early and faster death than those of the ST Ia isolates. However, there existed no significant difference in the cumulative mortalities of 14 dpi between the two serotypes. The LD50of the ST Ia and ST III isolates were at 2.5 × 104and 1.9 × 104CFU/fish, respectively. The clinical signs and gross and histopathological lesions of experimentally infected fish between serotype Ia or III were also similar. The circulation of S. agalactiaeST Ia and ST III in Vietnam wasreported for the first time in this study and their pathogenicity was characterized inlaboratory scale.

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