Received: 17-04-2023
Accepted: 29-08-2023
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Production of in vitroEmbryo from FrozenPorcine Oocytes by Virtification Method
,
Nguyen Hue Linh
1
,
Nguyen Thi Ngoc Anh
1
Keywords
Maturation, vitrification method, porcine oocytes
Abstract
The study was conducted to evaluate the maturation rate and development of frozen porcine oocytes by vitrification method. After thawing, porcine oocytes were matured in Porcine Oocyte Maturation medium (POM), then fertilized with frozen-thawed semen and cultured in Porcine Zygote Medium (PZM3); non-frozen porcine oocytes were cultured in the same procedure as control. The results showed the maturation rate (64.97 ± 3.42%), fertilization rate (74.37 ± 2.08%) and blastocyst formation rate (9.61±1.81%) of frozen-thawed porcine oocytes. This appeared that for the first time in Vietnamimmature pig oocytes were frozen by vitrification and developed to blastocyst stage after thawing, although these results were lower than those of the group of eggs without cryopreservation with maturation and embryogenesis rates of 84.30 ± 1.90% and 46.68 ± 5.86% and 22.96 ± 3.53%, respectively. This study shows that vitrification had great potential in the application of immature pig oocytes.
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