Received: 23-06-2020
Accepted: 29-09-2020
DOI:
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Resistance to Orange-Rot Fungus Penicillium digitatumof Culture Solutionsfrom Trichoderma
,
Tran Bao Tram
1
,
Nguyen Thi Hien
1
,
Nguyen Xuan Canh
2
,
Thai Hanh Dung
3
,
Hoang Phuong Thao
3
,
Nguyen Nhat Tan
3
,
Nguyen Tran Ha Anh
3
,
Tran Van Tuan
3
Keywords
Orange-rot, antifungal activity, Penicilliulm digitatum, Trichoderma asperellum
Abstract
Trichodermais a genus of fungus potential for probiotic preparation because it is safe and has strong resistance against many fungal species.The objective of this study was to evaluate the P. digitatumantifungal ability of Trichodermaculture in order to find the potential fungal strains for the production of P. digitatumantifungal probiotics that cause orange rot.In this study, twenty Trichodermasamples (Tr.HG1 - Tr.HG20)were isolated from orange-growing soil in Ha Giang province, of which Tr.HG6 and Tr.HG11 samples were assessed as being highly resistant to P. digitatumcausing orange rot(The diameters of the resistant zones were 64,0 ± 1,0 and 45,3 ± 1,5 mm, respectively). Based on the morphological characteristics and sequence of ITS region of rDNA, Tr.HG6 and Tr.HG11 samples were identified as Trichoderma asperellum. On PDB media, after 72 h of incubation at 30°C, cultures of two strains of T. asperellum Tr.HG6 and Tr.HG11 showed the strongest P. digitatum resistance(The diameters of the resistant zones were 64.0 and 45.3 mm, respectively). The culture solution of T. asperellum Tr.HG6 showed high thermal stability and had ability to keep its high activity at 50°C. Simultaneously, the culture solution of Tr.HG6 showed the inhibitory activity of P. digitatumon orange. This study was able to select twoT. asperellumfungal strains, viz. Tr.HG6 and Tr.HG11, that had strong ability to resist fungi with great potential for applying into probiotic preparation.
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