Detection and Identification of Virus Causing Mosaic onLettuce in SomeProvinces of the Red River Delta

Received: 03-04-2018

Accepted: 14-06-2018

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Issue: Vol. 16 No. 4 (2018)

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NÔNG HỌC

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Huy, N., Hong, N., & Anh, B. (2024). Detection and Identification of Virus Causing Mosaic onLettuce in SomeProvinces of the Red River Delta. Vietnam Journal of Agricultural Sciences, 16(4), 291–300. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/452

Detection and Identification of Virus Causing Mosaic onLettuce in SomeProvinces of the Red River Delta

Nguyen Duc Huy (*) 1 , Nguyen Thi Thanh Hong 1 , Bui Phuong Anh 2

  • 1 Khoa Nông học, Học viện Nông nghiệp Việt Nam
  • 2 Lớp K57BVTVA, Khoa Nông học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Lactuca sativa, potyvirus, Lettuce mosaic virus, indicatorplants, RT-PCR

    Abstract


    The aim of this studywasto identify potyviruses from lettuce leaf samplesshowing virus-like symptom collected from sixprovinces in theRed River Delta. The leafsamples were inoculated onto indicator plants such as Chenopodium quinoa, C. amaranticolorand Nicotiana benthamianafor examining the presence of the viruses. Furthermore, total RNAs wereextracted from the leafsamples and amplified by RT-PCR using universal primer pairs, CI-For and CI-Rev, for the detection of potyvirus. After inoculation,indicator plants showed yellow spot and systemic infection on C. quinoa, C. amaranticolorand mosaic on N. benthamiana,indicatingthat lettuce the leaves were infected withvirus. RT-PCR amplificationof about 0.7 kb revealedthat the leaf samples were infected by genus potyvirus. Sequencingof RT-PCR products showed 99.0% nucleotide identiy of C terminus of the cylindrical inclusion (CI)withLettuce mosaic virus(LMV). LMV infectsGomphrena globose, Datura metel, Pisum sativumand Solanum lycopersicum in the pathogenicity test.This is the first report onLMV in Vietnam.

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