Correlation between Density of Vibriospp. and Salinity in Shrimp Pond

Received: 09-01-2017

Accepted: 24-04-2017

DOI:

Views

2

Downloads

0

Issue: Vol. 15 No. 4 (2017)

Section:

CHĂN NUÔI – THÚ Y – THỦY SẢN

How to Cite:

Hanh, T., Van, K., Le, H., & Van, P. (2024). Correlation between Density of Vibriospp. and Salinity in Shrimp Pond. Vietnam Journal of Agricultural Sciences, 15(4), 355–361. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/367

Correlation between Density of Vibriospp. and Salinity in Shrimp Pond

Truong Thi My Hanh (*) 1 , Kim Van Van 2 , Huynh Thi My Le 3 , Phan Thi Van 1

  • 1 Viện nghiên cứu Nuôi trồng thủy sản
  • 2 Khoa Thủy sản, Học viện Nông nghiệp Việt Nam
  • 3 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Correlation, Vibriospp., AHPND

    Abstract


    This study investigated the relationship between the salinity and development of Vibriobacteria inthefield and laboratory conditions. A totalof 90 water samples were collected from 9 ponds at timescorresponding tof 10 different salinities. Three strains of bacteria.V. parahaemolyticus, V. vulnificus14.2 and V. vulnificuswere experimentally culured in different conditions of salinity (3, 15, 25, 35 and 40 ‰). V. choleraewas cultured in sterilized shrimp pond and distilled water. The densities of bacteria in shrimp pond water samples and cultured mediawere measured. Research results showed that there was a correlation between bacterial density and salinity inpond water environment as well as in experimental culture media. The correlation coefficient betweenVibriospp. and salinity in the pond was 0.75 (P <0.05); betweenV. vulnificus17.5 and the salinity was 0.69 (P <0.05). V. cholearedensity in sterile water pond was (7,5 x 106), significantly higher than that in distilled water (1.3 x 102colonies/ml). However, the growth of V. vulnificus14.2 and V. parahaemolyticuswas not significantly correlated withsalinity ( r =0.19 and 0.17), respectively. Research showedthat salinity can have an important role in disease outbreak caused by Vibrio.

    References

    Barbieri, E., Falzano, L., Fiorentini, C., Pianetti, A., Baffone, W., Fabbri, A., Matarrese, P., Casiere, A., Katouli, M., Kühn, I., Möllby, R., Bruscolini, F., Donelli, G. (1999). Occurrence, diversity, and pathogenicity of halophilic Vibrio spp. and non-O1 Vibrio cholerae from estuarine waters along the Italian Adriatic coast. Appl. Environ. Microbiol., 65: 2748-2753.

    Chen, L.L., Lo, C.F., Chiu, Y.L., Chang, C.F., Kou, G.H. (2000). Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata. Dis. Aquat. Organ., 40: 157-161. doi:10.3354/dao040157

    Sobrinho D.C., P., Destro, M.T., Franco, B.D.G.M., Landgraf, M. (2010). Correlation between environmental factors and prevalence of Vibrio parahaemolyticusin oysters harvested in the southern coastal area of Sao Paulo state, Brazil. Appl. Environ. Microbiol. 76, 1290-1293. doi:10.1128/AEM.00861-09

    Deepanjali, A., Kumar, H.S., Karunasagar, I., Karunasagar, I. (2005). Seasonal variation in abundance of total and pathogenic Vibrio parahaemolyticusbacteria in oysters along the southwest coast of India. Appl. Environ. Microbiol. 71, 3575-3580. doi:10.1128/AEM. 71.7.3575-3580.2005

    DePaola, A., Nordstrom, J.L., Bowers, J.C., Wells, J.G., Cook, D.W. (2003). Seasonal abundance of total and pathogenic Vibrio parahaemolyticusin Alabama oysters. Appl. Environ. Microbiol. 69, 1521-1526. doi:10.1128/AEM.69.3.1521-1526. 2003.

    Eiler, A., Johansson, M., Bertilsson, S. (2006). Environmental influences on Vibriopopulations in northern temperate and boreal coastal waters (Baltic and Skagerrak Seas). Appl. Environ. Microbiol. 72, 6004-6011. doi:10.1128/AEM. 00917-06.

    Flegel, T.W. (2012). Historic emergence, impact and current status of shrimp pathogens in Asia. J. Invertebr. Pathol. 110, 166-73. doi:10.1016/j.jip. 2012.03.004.

    Han, J.E. (2017). Four AHPND strains identified on Latin American shrimp farms. http://advocate. gaalliance.org/four-ahpnd-strains-identified-on-latin-american-shrimp-farms/.

    Høi, L., Larsen, J.L., Dalsgaard, I., Dalsgaard, A., 1998. Occurrence of Vibrio vulnificusbiotypes in Danish marine environments. Appl. Environ. Microbiol., 64: 7-13.

    Hong, X., Lu, L., Xu, D. (2016). Progress in research on acute hepatopancreatic necrosis disease (AHPND). Aquac. Int. doi:10.1007/s10499-015-9948-x.

    Kaspar, C.W., Tamplin, M.L. (1993). Effects of temperature and salinity on the survival of Vibrio vulnificusin seawater and shellfish. Appl. Environ. Microbiol., 59: 2425-2429.

    Kim, Y.W., Lee, S.H., Hwang, I.G., Yoon, K.S. (2012). Effect of temperature on growth of Vibrioparaphemolyticus and Vibrio vulnificusin flounder, salmon sashimi and oyster meat. Int. J. Environ. Res. Public Health 9, 4662-4675. doi:10.3390/ijerph9124662

    Kondo, H., Van, P.T., Dang, L.T., Hirono, I. (2015). Draft Genome Sequence of Non-Vibrio parahaemolyticusAcute Hepatopancreatic Necrosis Disease Strain KC13.17.5, Isolated from Diseased Shrimp in Vietnam. Genome Announc 3. doi:10.1128/genomeA.00978-15

    Kriem, M.R., Banni, B., El Bouchtaoui, H., Hamama, A., El Marrakchi, A., Chaouqy, N., Robert-Pillot, A., Quilici, M.L. (2015). Prevalence of Vibriospp. in raw shrimps (Parapenaeus longirostris) and performance of a chromogenic medium for the isolation of Vibriostrains. Lett. Appl. Microbiol. 61, 224-230. doi:10.1111/lam.12455

    Lê Hồng Phước, P. Sorgeloos, P.B. (2012). Ảnh hưởng của độ mặn lên sự phát triển của các loài vi khuẩn thuộc giống Vibriotrên môi trường chọn lọc và không chọn lọc. Báo cáo tổng kết, Viện Nghiên cứu Nuôi trồng Thủy sản II 1-42.

    Leano, E.. and C.V.M. (2012). Early Mortality Syndrome Threatens Asia’s Shrimp Farms. Glob. Aquac. Advocate, 38:39.

    Lipp, E.K., Rodriguez-Palacios, C., Rose, J.B. (2001). Occurrence and distribution of the human pathogen Vibrio vulnificusin a subtropical Gulf of Mexico estuary. Hydrobiologia 460, 165-173.

    Martinez-Urtaza, J., Blanco-Abad, V., Rodriguez-Castro, A., Ansede-Bermejo, J., Miranda, A., Rodriguez-Alvarez, M.X. (2012). Ecological determinants of the occurrence and dynamics of Vibrio parahaemolyticusin offshore areas. ISME J. 6, 994-1006. doi:10.1038/ismej.2011.156

    Messelhäusser, U., Colditz, J., Thärigen, D., Kleih, W., Höller, C., Busch, U. (2010). Detection and differentiation of Vibriospp. in seafood and fish samples with cultural and molecular methods. Int. J. Food Microbiol. 142, 360-364. doi:10.1016/j.ijfoodmicro. 2010.07.020

    Motes, M.L., DePaola, A., Cook, D.W., Veazey, J.E., Hunsucker, J.C., Garthright, W.E., Blodgett, R.J., Chirtel, S.J. (1998). Influence of water temperature and salinity on Vibrio vulnificusin Northern Gulf and Atlantic Coast oysters (Crassostrea virginica). Appl. Environ. Microbiol. 64, 1459-1465.

    Nunan, L., Lightner, D., Pantoja, C., Gomez-Jimenez, S. (2014). Detection of acute hepatopancreatic necrosis disease (AHPND) in Mexico. Dis. Aquat. Organ. 111, 81-6. doi:10.3354/dao02776

    Pfeffer, C.S., Hite, M.F., Oliver, J.D. (2003). Ecology of Vibrio vulnificusin estuarine waters of eastern North Carolina. Appl. Environ. Microbiol. 69, 3526-3531. doi:10.1128/AEM.69.6.3526

    Phạm Thi Tuyết Ngân, N.H.T. (2010). Biến động mật độ vi khuẩn hữu ích trong ao nuôi tôm sú (Penaeus monodon) thâm canh. Tập chí khoa học, Đại học Cần Thơ, 12: 166-176.

    Phan Thị Vân, Bùi Ngọc Thanh, Phạm Thị Yến, N.T.H. (2013). Xác định nguyên nhân gây bệnh hoại tử gan tụy cấp trên tôm nuôi tại miền Bắc. Báo cáo tổng kết đề tài , Viện Nghiên cứu Nuôi trồng Thủy sản I 1-65.

    Randa, M.A., Polz, M.F., Lim, E. (2004). Effects of temperature and salinity on Vibrio vulnificuspopulation dynamics as assessed by quantitative PCR. Appl. Environ. Microbiol. 70, 5469-5476. doi:10.1128/AEM. 70.9.5469-5476.2004

    Singleton, F.L., Attwell, R., Jangi, S., Colwell, R.R. (1982). Effects of temperature and salinity on Vibriocholerae growth. Appl. Environ. Microbiol. 44, 1047-1058.

    Tran, L., Nunan, L., Redman, R.M., Mohney, L.L., Pantoja, C.R., Fitzsimmons, K., Lightner, D. V. (2013). Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Dis. Aquat. Organ. 105, 45-55. doi:10.3354/dao02621

    Wetz, J.J., Blackwood, A.D., Fries, J.S., Williams, Z.F., Noble, R.T. (2014). Quantification of Vibrio vulnificusin an Estuarine Environment: A Multi-Year Analysis Using QPCR. Estuaries and Coasts 37, 421-435. doi:10.1007/s12237-013-9682-4

    Wright, A.C., Hill, R.T., Johnson, J.A., Roghman, M.C., Colwell, R.R., Morris, J.G. (1996). Distribution of Vibrio vulnificusin the Chesapeake Bay. Appl. Environ. Microbiol., 62: 717-724.

    Cục Thú y (2015). Báo cáo kết quả công tác Thú y năm 2015. Báo cáo cuối năm 2015, tr. 1-32.