Establishment of Plant Regeneration Protocol for Cassava (Manihot esculenta Crantz) via Somatic Embryogenesis from Immature Leaves

Received: 25-07-2014

Accepted: 08-11-2016

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Issue: Vol. 15 No. 1 (2017)

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NÔNG HỌC

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Lan, D., Binh, L., Son, L., & Ha, C. (2024). Establishment of Plant Regeneration Protocol for Cassava (Manihot esculenta Crantz) via Somatic Embryogenesis from Immature Leaves. Vietnam Journal of Agricultural Sciences, 15(1), 1–6. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/345

Establishment of Plant Regeneration Protocol for Cassava (Manihot esculenta Crantz) via Somatic Embryogenesis from Immature Leaves

Do Hai Lan (*) 1 , Le Tran Binh , Le Van Son , Chu Hoang Ha

  • 1 Đại học Tây Bắc

    • Keywords

    Cassava (Manihot esculentaCrantz), callus culture, immature leaf, somatic embryos, regeneration

    Abstract


    The present study aimed at regenerating cassava(Manihot esculenta Crantz) via somatic embryogenesis. The explants used were immature leaves harvestedfrom 2-3-week old in vitroplantsderived from two cassava cultivars, KM94 andKM140. Somatic embryogenesis was achieved with high frequencies usingMS medium supplemented with a wide range of picloram concentrations. The results showed that the highest rate of somatic embryo formation for both cultivars was obtained when cultured in MS mediumcontaining 12 mg/l picloram. Although the number of embryos per explant was similar between two cultivars at4 weeksof culture, KM94 cultivar gave a higher rate (81,4 ± 1,7%) than KM140 cultivar (70,4 ± 2,9%). Somatic embryos were subsequently transferred to MS medium supplemented with 0.3 mg/l BAP for plantlet regeneration. All shoots with 1.0-1.5 cmin length transferredto - hormone-free MS medium formed roots in 2 weeks. Rooted plantlets were transplanted toa mixture of rice husk (40%) and sandy soil (60%) in greenhouse. This protocol required 16 to 18 weeks and was entirely appropriate for mass production of various cassava genotypes and further genetic transformation experiments.

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