Isolation and Screening of Extracellular Phytase Producing Bacteria

Received: 15-05-2013

Accepted: 16-08-2013

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Giang, N., Dao, T., Hieu, D., Bich, D., Tu, T., & Hang, B. (2024). Isolation and Screening of Extracellular Phytase Producing Bacteria . Vietnam Journal of Agricultural Sciences, 11(4), 558–564. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1639

Isolation and Screening of Extracellular Phytase Producing Bacteria

Nguyen Van Giang (*) 1 , Tran Thi Dao 2 , Dinh Quang Hieu 2 , Dang Ngoc Bich 2 , Tran Thi Cam Tu 2 , Bui Thi Hang 2

  • 1 Khoa Công nghệ sinh học, Trường Đại học Nông nghiệp Hà Nội
  • 2 Khoa Công nghệ sinh học - Trường Đại học Nông nghiệp Hà Nội
  • Keywords

    Bacillus spp., phytase, PSM

    Abstract


    Phytases are enzymes hydrolyzing phytic acid to release inorganic phosphate and myo-inositol.Phytase produced by microorganisms have potential industrial applications. The supplementation of animal feed with phytases reduces the cost of diets by removing or reducing the need for supplemental inorganic phosphate and increases the bioavailability of phosphorous in monogastric animals. In this study, soil close to pig and chicken farms at Ha Noi university of agriculture was used as a source material for isolating phytase producing bacteria. On PSM medium, six bacterial isolates having potential phytase production were isolated. Among these, M4 isolate showed maximum phytase activity. M4 isolate was biochemically characterized as Bacillus spp.

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