Assessing Genetic Diversity Tomato and Yellow Leaf Curl Virus (TYLCV) Resistance Gene ofTomato Varieties by DNAMarkers

Received: 15-08-2014

Accepted: 22-01-2015

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Issue: Vol. 13 No. 1 (2015)

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NÔNG HỌC

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Canh, D., Hai, T., Minh, N., & Thuy, D. (2024). Assessing Genetic Diversity Tomato and Yellow Leaf Curl Virus (TYLCV) Resistance Gene ofTomato Varieties by DNAMarkers. Vietnam Journal of Agricultural Sciences, 13(1), 1–11. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1516

Assessing Genetic Diversity Tomato and Yellow Leaf Curl Virus (TYLCV) Resistance Gene ofTomato Varieties by DNAMarkers

Doan Xuan Canh (*) 1 , Tong Van Hai 2 , Nguyen Hong Minh 2 , Doan Thi Thanh Thuy 1

  • 1 Viện Cây lương thực Cây thực phẩm
  • 2 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Abstract


    Tomato is apopularly important vegetable in the world and Vietnam. Assessing genetic diversity and detcetion of genes for disease resistance of the existing germplasm of tomato are usefull for development ofnew varieties with high yield, good qualityand disease resistance. In this study,10 SSR markersand four markers related to TYLCV resistance genes (Ty1, Ty2and Ty3) were usedto analyse genetic diversityand identify the TYLCV genes, respectively, of26 of tomatoes accessions available at food crops research institute. The results showed, that only 5 marker showedpolymorphism.26 accessions were grouped into 5 clusters with similarity coefficients threshold of 0.7 by using NTSYSpc 2.1 software. The information found in this study is very important fornew tomato hybrid breeding programs.Only four of 26 accessions carriedTy1andthe genesTy2 and Ty3 were not detected

    References

    Castro, Ana Pérez de, Blanca, José Miguel, Díez, María José, & Vinals, Fernando Nuez (2007). Identification of a CAPS markertightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato. Eur JPlant Pathol; 117: 347-356.

    F. James Rohlf (2000). NTSYSpc Numerical Taxonomy and Multivariate Analysis System Version 2.1

    Han, c., Jianjun, s., Linshan, w., Peng, t. & Yan, q. 2012. Establishment of CAPS Molecular Marker forTy-1Gene Resistant to Tomato Yellow Leaf Curl Disease. Chinese Agricultural Science Bulletin, 28: 195-200.

    Hanson, P.M, Green, S.K, & Kuo, G. (2006). Ty-2 gene on chromosome 11 conditioning geminivirus resistance in tomato. Report of the Tomato Genetics Cooperative, 56: 17-18.

    Doyle J. J., và J. L. Doyle (1990). A rapid total ADN preparation procedure for fresh plant tissue. Focus, 12: 13-15.

    Kaemmer D, Weising K, Beyermann B, Börner T, Epplen J T, Kahl G. 1995. Oligonucleotide fingerprinting of tomato ADN. Plant Breeding, 114: 12-17.

    Meng Fan-juan, XU Xiang-yang, Huang Feng-lan, LI Jing-fu (2010). Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR. Agricultural Sciences in China 9(10): 1430-1437.

    Nei M (1973). Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci., 70: 3321-3323.

    Ji, Yuanfu, Salus, Melinda S., Betteray, Bram van, Smeets, Josie, Jensen, Katie S., Martin, Christopher T., Mejía, Luis, Scott, Jay W., Havey, Michael J., & Maxwell, Douglas P. (2007). Co-dominant SCAR Markers for Detection of the Ty-3 and Ty-3a Loci from Solanum chilense at 25 cM of Chromosome 6 of Tomato. Report of the Tomato Genetics Cooperative, 57: 25-28.

    Ji, Yuanfu, Schuster, David J., & Scott, Jay W. (2007). Ty-3, a begomovirus resistance locus near the tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato. Molecular Breeding, 20(3): 271-284.

    Parmar Pritesh, Vishal P. Oza, Vaishnavi Chauhan, A.D. Patel, K.B. Kathiria, and R.B. Subramanian (2010). Genetic Diversity and ADN Fingerprint Study of TomatoDiscerned by SSR Markers. International Journal of Bi otechnology and Biochemistry, 6(5): 657-666.

    Dương Kim Thoa (2012). “Nghiên cứu nguồn vật liệu khởi đầu cho tạo giống cà chua ưu thế lai phục vụ chế biến ở Đồng bằng sông Hồng”, Luận ántiến sĩ Nông nghiệp, Trường Đại học Nông nghiệp Hà Nội.