Received: 09-11-2015
Accepted: 11-03-2016
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Effect of Colchicine Concentration and Duration of Treatment on Polyploidy Induction in Shallot (Allium cepaL., Aggregatum Group)
Keywords
Colchicine, polyploidy, shallot, tetraploid
Abstract
Local shallot (Allium cepaL. ), Aggregatum group) variety grown inKinh Mon, Hai Duong was treated with colchicine in order to produce polyploid materials for high-quality shallot breeding in Viet Nam. The research was carried out at Faculty of Agronomy, Vietnam National University of Agriculture. The shallot bulbs were immersedincolchicine solution with different concentrations (0,2%; 0,4%; 0,6%; 0,8%; 1,0%; 1,2%; 1,4%; 1.6%; 1,8%; 2,0%)for oneto tendays. The results revealed that colchicine treatment at concentration 1,0% for 6 days was most suitable for inducing tetraploid shallot plants with survival rate of 80%. Thepercentage of tetraploids was20% in total treated bulbs. Treatment at higher concentration and longer duration increased the percentage of dead plants up to 100%. Thirty six tetraploid plants of shallot were produced.
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