DEVELOPMENT OF RICE LINES (Oryza sativaL.) TOLERANT TO SUBMERGENCE VIA Sub1GENE INTRODUCTION INTO LANDRACES AND ELITE BREEDING LINES

Received: 08-10-2015

Accepted: 11-03-2016

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Issue: Vol. 14 No. 3 (2016)

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NÔNG HỌC

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Lang, N., Hoa, N., Ha, P., Hieu, N., Huong, N., Buu, B., … Wassmann, R. (2024). DEVELOPMENT OF RICE LINES (Oryza sativaL.) TOLERANT TO SUBMERGENCE VIA Sub1GENE INTRODUCTION INTO LANDRACES AND ELITE BREEDING LINES. Vietnam Journal of Agricultural Sciences, 14(3), 307–320. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1417

DEVELOPMENT OF RICE LINES (Oryza sativaL.) TOLERANT TO SUBMERGENCE VIA Sub1GENE INTRODUCTION INTO LANDRACES AND ELITE BREEDING LINES

Nguyen Thi Lang (*) 1 , Nguyen Thanh Hoa 1 , Pham Thi Thu Ha 1 , Nguyen Van Hieu 1 , Nguyen Ngoc Huong 1 , Bui Chi Buu 2 , Russell Reinke 3, 4 , Tran Bao Toan 5 , Abdelbagi M Ismail 3 , Reiner Wassmann 3

  • 1 Cuu Long Delta Rice Research Institute (CLRRI), Thoi Lai, Can Tho, Viet Nam
  • 2 Institute of Agricultural Science for Southern Viet Nam (IAS), Viet Nam
  • 3 International RiceResearch Institute, DAPO 7777, Metro Manila, Philippines
  • 4 Temperate Rice Breeder – IRRI andNICS project, Suwon 441-857, Republic of Korea
  • 5 Biotechnology PCR Company, Can Tho, Viet Nam

    • Keywords

    chromosome, molecular markers, submergence tolerance, QTL

    Abstract


    Development of rice genotypes tolerant to complete submergence in the Mekong Delta was carried out for three continuous years by CLUES Project fund. Eighty five high-yielding cultivars and eighty four progenies from backcrossing population (BC3F3) of OM1490/IR64 Sub1were used to study the yield components and submergence tolerance. Phenotyping was implemented at three stages: seedling, tillering and heading. Different segregation ratio and phenotypical variation indicated the complex genetic background. All genotypes have been evaluated under both submergence and non-submergence treatments. Correlation coefficient between the survival rate (%) and tiller number /10 hills was positively significant of 0.8880**. Marker-assisted backcrossing (MAB) was exploited on chromosomes 9 to address ten molecular markers (SSRs). Of them, only three polymorphic SSRs really linked to the target QTLs. Marker-assisted selection helped identify elite lines, which would be breeding materials via three molecular markers on the target region viz. RM3269, RM5304 and RM1367 on chromosome 9. Three selected offspring lines from BC3F3population of OM1490/IR64 Sub1as 26, 38 and 50 exhibited good adaptation to submergence.

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