Functional Roleof Mould Strains in Production of Rice Wine

Received: 07-03-2012

Accepted: 17-05-2012

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KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Hoa, N., & Nghiem, D. (2024). Functional Roleof Mould Strains in Production of Rice Wine. Vietnam Journal of Agricultural Sciences, 10(3), 503–516. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/13

Functional Roleof Mould Strains in Production of Rice Wine

Nguyen Ngoc Hoa (*) 1 , Dang Xuan Nghiem 2

  • 1 Khoa Công nghệ sinh học, Trường Đại học Nông nghiệp Hà Nội
  • 2 Khoa Công nghệ Sinh học, Đại học Nông nghiệp Hà Nội
  • Keywords

    Ethanol fermentation, Mould, Rice wine, Rice wine starter, Saccharification

    Abstract


    This investigation on the possible role of mould in production of traditional rice wine was carried out with the 4 candidate mould strains which were selected from 21 mould strains isolated from traditional rice wine starters and traditional soysauce starters. The 4 mould strains were preliminarily identified and named as M3 (Rhizopus spp.), M10 (Rhizopus spp.), M16 (Aspergillus oryzae), and M18 (Endomycopsisfibuliger). M10, M16 and M18 strains expressed very strong extracellular enzyme activities (amylase, protease, cellulase), while M3 strain had weak extracellular protease and cellulase activity and no extracellular amylase. Noteably, the yield of rice wine of the traditional alcoholic fermentation was not increased when large amounts of the biomass of the 4 isolated mould strains were added. There was a strong correlation between the pH of fermention liquid and the final yield of wine. The lower the pH of fermentation liquid was, the lower the yield of wine. Moreover, all 4 mould strains were determined to have no role in the formation of aldehyde, methanol and higher alcohol in rice wine products.

    References

    Barnett, J.A., Payne, R.W. and Yarrow, D (2000). Yeasts: Characteristics and Identification. 3rd edn. Cambridge, UK: Cambridge University Press.

    Bernfeld (1955). Amylase alpha and beta. Methods Enzymol. 1:149-158.

    Êgôrôv, N. X (1983). Thực tập vi sinh vật học. Nhà xuất bản “MIR” Maxcova (Nguyễn Lân Dũng dịch).

    Phạm Thu Hà, Mauvais, G., Vergoignan, C., Cachon, R. Feron, Gilles (2010). Ảnh hưởng của việc thay đổi môi trường oxy hóa khử bằng sục khí đến tiêu thụ đường ở nấm men bia Saccharomyces cerevisiae. Tạp chí khoa học và phát triển Tập 8, số 2: 319 - 326. Trường đại học Nông nghiệp Hà Nội.

    Kammoun, R., Naili, B., Bejar, S (2008). Application of a statistical design to the optimization of parameters and culture medium for alphaamylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product). Bioresour Technol. 99, 5602-5609.

    Klich, M.A (2002). Identification of Common Aspergillus Species. Utrecht: Centraalbureau voor Schimmelcultures. The Netherlands.

    Kurtzman, C.P. and Fell, J.W (1998). The Yeasts, A Taxonomic Study, 4th edn. Amsterdam: Elsevier.

    Joosten, M. and Peeters, M (2010). Yeast and fermentation: the optimal pH level. Philips van Horne sg. Weert, The Netherlands.

    Li, H.F., Chi, Z.M., Wang, X.H., Duan, X.H., Ma, L.Y., Gao, L.M(2007). Purification and characterization of extracellular amylase from the marine yeast Aureobasidium pullulans N13d and its raw potato starch digestion. Enzyme Microb Technol. 40: 1006 - 12.

    Limtong, S., Sintara, S., Suwannarit, P. and Lotong, N. (2002). Yeast diversity in Thai traditional alcoholic starter. Kasetsart Journal of Natural Sciences. 36: 149 - 158

    Milsom, P.E., Meers, J.L (1985). Gluconic acids and itaconic acids. Compr. Biotechnol. 3: 681-700.

    Murashima, K., Nishimura, T., Nakamura, Y., Koga, J., Moriya, T., Sumida, N. 2002. Purification and characterization of new endo - 1,4 - β - D - glucanses from Rhizopus oryzae. Enzyme Micro Technol. 30: 319 - 26.

    Nguyen .T.P.D., Romboutsb, F.M., Nout, M.J.R (2006). Functionality of selected strains of mouldsand yeasts from Vietnamese rice winestarters. Food Microbiology. 23(4): 331-340.

    Nguyen.T.P.D (2004). Defined fungal stater granules for purple glutinous rice wine. Ph.D. thesis Wageningen University, Wageningen, the Netherlands.

    Lương Đức Phẩm, Nguyễn Thế Trang, Nguyễn Phương Nhuệ, Nguyễn Văn Hiếu (2000). Tuyển chọn các chủng vi sinh vật từ các sản phẩm lên men truyền thống. Báo cáo khoa học Hội nghị Sinh học Quốc gia. NXB Đại học Quốc gia Hà Nội. 139-142.

    Huỳnh Xuân Phong, Huỳnh Ngọc Thanh Tâm, Hà Thanh Toàn, Ngô Thị Phương Dung (2007). Khảo sát chất lượng men làm rượu và rượu Xuân Thạnh, Trà Vinh.Tạp chí Khoa học. 7: 121-129.

    Schägger and von Jagow (1987). Tricine-Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis for the Separation of Proteins in the Range from 1 to 100 kDa. Analytical biochemistry. 166: 368-379

    Schipper, M.A.A. and Stalpers, J.A (1984). A revision of the genus Rhizopus. 2. The Rh. microsporus-group. Stud. Mycol. Baarn. 25: 20-34.

    Zhan, Y.Z., Bo, J (2007). Production of lactic acidfrom renewable materials by Rhizopusfungi. Biochemical Engineering Journal. 35 (3): 251-263.

    Zhang, L., Chi, Z.M (2007). Screening and identification of a cellulase producting marine yeast and optimization of medium and cultivation conditions for cellulase production. J Ocean Univer China. 37: 101 - 8.