Received: 16-08-2023
Accepted: 05-01-2024
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Study on In vitroPropagation of Purple petunia (Petunia hybrida Hort.)
,
Pham Thi Huyen Trang
1
,
Nguyen Thi Thuy Hanh
2
Keywords
Activated charcoal, BA, Petunia hybrida Hort., tissue culture
Abstract
Purple petunia (Petunia hybrida Hort.), belonging to the Solanacea family, is a popular and high - value potted ornamental plant on the flower market. Therefore, in vitro propagation research of Purple petunia has been carried out in order to produce large numbers of genetically uniform and pathogen-free plantlets to meet market demand. All experiments were arranged in a randomized complete design (RCD) with three replicates. The results showed that Murashige and Skoog (MS) solid medium supplemented with 0.3 mg/l BA was the most suitable medium for shoot regeneration and multiplication of Purple petunia, with a high multiplication rate (19.78 shoots/explant), and a shoot height of 2.36cm after 6 weeks of culture. The most suitable medium for Purple petunia shoot nursing was MS solid medium supplemented with 40 g/l sucrose, producinglarge and green shoot and leaves. The results also showed that the suitable medium for the rooting stage and acclimatization was MS solid medium supplemented with 0.1 g/l activated charcoal (AC), resulting in the root formation rate of 100%; 12.83 roots/plantlet; 2.28 cm in root length with white, long and big roots. After 2 weeks of acclimatization in the net house, plantlets derived from MS solid rooting medium supplemented with 0.1 g/l AC had a survival rate of 100% with 6.47cm in plant height and 15.07 leaves/plant.
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