Effects of Culture Conditions on Mycelial Growth and Fruiting Bodies of Trametes versicolorMushroom Tra-6 Strain

Received: 03-10-2023

Accepted: 25-12-2023

DOI:

Views

0

Downloads

0

Issue: Vol. 22 No. 1 (2024)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Ha, T., Giang, N., Trinh, N., & Phuong, N. (2024). Effects of Culture Conditions on Mycelial Growth and Fruiting Bodies of Trametes versicolorMushroom Tra-6 Strain. Vietnam Journal of Agricultural Sciences, 22(1), 70–81. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1245

Effects of Culture Conditions on Mycelial Growth and Fruiting Bodies of Trametes versicolorMushroom Tra-6 Strain

Tran Thu Ha (*) 1 , Nguyen Van Giang 2 , Nguyen Duy Trinh 1 , Nguyen Thi Phuong 2

  • 1 Trung tâm Nghiên cứu và Phát triển Nấm, Viện Di truyền Nông nghiệp
  • 2 Học viện Nông nghiệp Việt Nam

    • Keywords

    Turkey tail, Trametes versicolor, mycelium, fruiting body, substrate

    Abstract


    Turkey tail mushroom (Trametes versicolor) is a medicinal mushroom used as a health-promoting pharmaceutical, extending life, and adjuvant therapy for cancer patients. The mycelia and fruiting bodies of the Turkey tail mushroom contain various biologically active compounds such as polysaccharides, glucans, phenolics, flavonols, coumarins, isoflavonoids, triterpenoids, and sterols. This study was carried out to choose the optimal culture conditions, including culture pH, temperature, medium, and substrate composition, for the growth of the mycelia and fruiting bodies of the T. versicolor mushroom Tra-6 strain, collected in Lao Cai, Vietnam. The experimental factors were examined individually in a randomized complete block design. Research results show that the mycelia of the Turkey tail mushroom Tra-6 strain quickly grew on MCM and YEA media at pH 8 and 25 ± 1C. Sucrose, yeast extract, MgSO4.7H2O, KCl, and KH2PO4 were the optimal carbon, nitrogen, and mineral salt sources for this strain, respectively. Tra-6 strain had the ability to form fruiting bodies when growing on substrate from agricultural by-products. The highest yield of 44.4kg dry mushroom per ton of dry substrate was obtained on a substrate consisting of 55% sawdust, 24% crushed corncobs, 20% rice bran, and 1% CaCO3 powder.

    References

    Bains A., Chawla P., Kaur S., Najda A., Fogarasi M. & Fogarasi S. (2021). Bioactives from Mushroom: Health Attributes and Food Industry Applications. Materials. 14: 2-16.

    Chauhan R. (2016). Optimization of physical parameters for the growth of a white rot fungus- Trametes versicolor. International Journal Of Information Research And Review. 3(11): 3125-3128.

    Elkhateeb W.A., Elnahas M.O., Thomas P.W & Daba G.M. (2020).Trametes Versicolorand Dictyophora IndusiataChampions of Medicinal Mushrooms. Open Access Journal of Pharmaceutical Research. 4(1): 1-7.

    Gonkhom D., Luangharn T., Hyde K.D., Stadler M. & Naritsada Thongklang N. (2022). Optimal conditions for mycelial growth of medicinal mushrooms belonging to the genus Hericium. Mycological Progress. 21: 82.

    Guerrero D.G., Martinez V.E. & De La Almaraz R.T. (2011). Cultivation of Trametes versicolorin Mexico. Michael Fajardo - Logistics Analyst. 23: 55-58.

    HeZ., LinJ.,HeY. & Liu. S. (2022). Polysaccharide-Peptide from Trametes versicolor: The Potential Medicine for Colorectal Cancer Treatment. Biomedicines.10(11): 2841.

    Itoo Z.A. & Reshi Z.A. (2014). Effect of different nitrogen and carbon sources and concentrations on the mycelial growth of ectomycorrhizal fungi under in-vitroconditions. Scandinavian Journal of Forest Research. 29: 619-628.

    Jo W.S., Kang M.J., Choi S.Y., Yoo Y.B., Seok S.J. & Jung H.Y. (2010). Culture conditions for mycelial growth of Coriolus versicolor. Mycobiology. 38: 195-202.

    Kunamneni A., Ballesteros A., Plou F.J. & Alcalde M. (2007). Fungal laccasea versatile enzyme for biotechnological applications. Communicating current research and educational topics and trends in applied microbiology. 1: 233-45.

    Laursen A. (2018). The Effect of Different Nitrogen Sources on Mycelial Growth of Oyster Mushroom, Pleurotus ostreatus. Bachelor Project in Biology. SLU- Department of Biosystems andTechnology. 61p.

    Luyen N.T., Ve V.L., Nguyen B.T.T., Nguyen H.T.T., Tran A.D., Ngo N.X. (2023). Optimization of mycelial growth and cultivation of wild Ganoderma sinense. Journal of Biotechnology, Computational Biology and Bionanotechnology. 104(1): 65-74.

    Mahadevan K. & Shanmugasundaram K. (2018). Comparative effect of different culture media on mycelial growth performance of Pleurotus sapidus. Journal of Pharmacognosy and Phytochemistry. 7(4): 874-878

    Mallikarjuna S.E., Ranjini A., Haware D.J., Vijayalakshmi M.R., Shashirekha M.N. & Rajarathnam S. (2013). Mineral Composition of Four Edible Mushrooms. Journal of Chemistry. pp. 1-5.

    Miles P.G. & Chang S.T. (1997). Mushroom biology: Concise basics and current developments. In: Miles, PG, editor. Mushroom Biology: Concise Basics and Current Developments. Singapore: World Scientific Publishing Company. 41.

    Miles P.G. & Chang S.T. (2004) Mushrooms: Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact. 2nded. Boca Raton, Florida: CRC Press.

    Nguyễn Thị Bích Thùy & Trịnh Tam Kiệt (2010). Nghiên cứu đặc điểm sinh học và Kỹ thuật nuôi trồng nấm Vân chi Trametes versicolor(Linnaeus. Fries) Pilat. Tạp chí Di truyền học và Ứng dụng. 6: 55-58.

    Nguyễn Văn Giang, Nguyễn Thị Phương Thảo, Trần Thu Hà & Nguyễn Duy Trình (2021). Ảnh hưởng của một số nguồn cacbon, nitơ và muối khoáng đến sinh trưởng hệ sợi nấm ngân nhĩ Tremella fuciformisTre-1. Tạp chí Khoa học Nông nghiệp Việt Nam. 19(11): 1522-1530.

    Nguyen B.T.T, Le V.V., Nguyen H.T.T, Nguyen L.T., Tran A.D. & Ngo N.X. (2021a). Successful Rescue of Wild Trametes versicolorStrains Using Sawdust and Rice Husk-based Substrate. Pakistan Journal of Biological Sciences. 24: 374-382.

    Nguyen B.T.T., Le V.V, Nguyen H.T.T., Nguyen L.T., Tran T.T.Y. & Ngo N.X. (2021b). Nutritional requirements for the enhanced mycelial growth and yield performance of Trametes versicolor. Journal of Applied Biology and Biotechnology. 9(1): 1-7

    Nguyen T.M. & Ranamukhaarachchi S.L. (2020). Effect of different culture media, grain sources and alternate substrates on the mycelial growth of Pleurotus eryngii andPleurotus ostreatus. Pakistan Journal of Biological Sciences. 23: 223-230

    Pasailiuk M.V., Sukhomlyn M.M. & Gryganskyi A.P. (2019). Patterns Of Hericium CoralloidesGrowth With Competitive Fungi. Czech Mycology. 71(1): 49-63.

    Petre M. (2016). Mushroom Biotechnology Developments and Applications. 1sted. Cambridge, Massachusetts: Academic Press.

    Sagar S., Thakur M., Sharma I. & Tripathi A. (2020). Optimization of mycelia growth parameters for Wild white rot fungi Trametes elegans and Trametes versicolor. Asia Life Sciences. 12(1): 4-17.

    Shroff S., Tiwari P.K., Kotasthane A.S. & Lakpale R. (2022). Effect of different media on mycelial growth & Chlamydospore production of paddy straw mushroom (Volvariella volvacea). The Pharma Innovation Journal. 11(6): 1932-1938.

    Ta K., Vyu B. & As. S. (2021) Review of the basic cultivation conditions influence on the growth of basidiomycetes. Current Research in Environmental & Applied Mycology. 11(1): 494-531.

    Trịnh Tam Kiệt (2012). Nấm lớn ở Việt Nam (Tập 2). Nhà xuất bản Khoa học Tự nhiên và Công nghệ, Hà Nội. 412tr.

    Veena S.S. & Pandey M. (2012). Physiological and cultivation requirements of Trametes versicolor, a medicinal mushroom to diversify Indian mushroom industry. The Indian Journal of Agricultural Sciences. 82: 672-675.

    Vũ Tuấn Minh & Lê Thị Thu Hường (2017). Nghiên cứu sự sinh trưởng, phát triển và năng suất nấm Vân chi (Trametes versicolor(L.) Pilat) trồng trên các loại giá thể tại Thừa Thiên Huế. Tạp chí Khoa học và Công nghệ. 1(1): 77-86.