Received: 30-08-2023
Accepted: 25-12-2023
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Isolation and Characterization of Lasiodiplodia theobromaeStrain Causing Dieback Disease on Grape in Ha Noi and Hung Yen
,
Dang Thi Thanh Tam
1
,
Do Thuy Hien
1
Keywords
Dieback, grape, Lasiodiplodia theobromae
Abstract
Dieback is one of the most common diseases on grape that is caused by fungi belonging to the Botryosphaeriaceae family. Among these fungi, Lasiodiplodia theobromaehas been identified as the most virulent species. Unfortunately, the current treatments for this disease are still challenging and not truly effective. This study was conducted to determine the causative agent and biological characteristics of the grape dieback-causing fungus in Hanoi and Hung Yen as a basis for guiding effective measures in the prevention and treatment of dieback. From grape samples showing dieback disease collected in Hanoi and Hung Yen, three fungal strains were isolated through culture on the PDA medium and these strains were subsequently re-infected onto leaves and stems of grapes. Among them, CK1 and DN1 strains showed similar mycelial characteristics and pathogenicity. Therefore, the CK1 strain was selected for further experiments. Through the molecular identification method with the ITS1/ITS4 primer pair, the CK1 strain was confirmed asLasiodiplodia theobromae and was named L. theobromae CK1. L. theobromae CK1 exhibited good growth on the PDA medium, and the optimal conditions for the strain were 35C and a pH in the range of 6-8. In addition, during its growth, this fungal strain was also capable of producing cellulase, xylanase, and pectinase.
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