Received: 25-10-2023
Accepted: 05-01-2024
DOI:
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How to Cite:
Evaluating Bactericidal Effect and Therapeutic Efficacy of Amoxicillin and Amoxicillin Combined with Clavulanic Acid on Streptococcus agalactiae Isolated from Diseased Tilapia
,
Tran Thi Diem Quynh
3
,
Dang Thi Hoa
3
,
Do Dinh Hung
3
,
Vo Van Viet
3
,
Nguyen Duc Chinh
3
,
Nguyen Thi Huong Giang
3
,
Doan Thi Nhinh
3
,
Ngo Phu Thoa
3
,
Kim Van Van
3
Keywords
Streptococcus agalactiae, amoxicillin, clavulanic acid, inhibition, treatment efficacy, tilapia
Abstract
The study evaluated and compared the bactericidal effect and treatment of the disease of tilapia caused by Streptococcus agalactiae with amoxicillin and amoxicillin combined with clavulanic acid. To conduct a study, 4 isolates of S. agalactiae isolated from diseased tilapia were selected for identification by biochemical methods and PCR assay. The inhibition ability of antibiotics was evaluated through the diffusion method on agar plate and the minimum inhibitory concentration (MIC). The treatment efficacy was evaluated 14 days post challenge. The results showed that amoxicillin combined with clavulanic acid revealed bigger inhibition zones. The minimum inhibitory concentration of amoxicillin was 2 µg/ml compared to that of only 0.25 µg/ml of amoxicillin and clavulanic acid. The results of treatment experiments showed that amoxicillin combined with clavulanic acid could yielded higher treatment efficacy (91.1%) than those treated with amoxicillin alone (77.8%). This is the first study to compare and evaluate the inhibitory and therapeutic efficacy of amoxicillin with and without clavulanic acid on pathogenic bacteria in aquaculture.
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