Factors Affecting the Efficiency of Rapid Vitrification of in vitroBovine Embryo

Received: 17-04-2023

Accepted: 29-08-2023

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Issue: Vol. 21 No. 9 (2023)

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CHĂN NUÔI – THÚ Y – THỦY SẢN

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Anh, N., Truong, N., Trang, N., Thanh, N., & Lanh, D. (2024). Factors Affecting the Efficiency of Rapid Vitrification of in vitroBovine Embryo. Vietnam Journal of Agricultural Sciences, 21(9), 1186–1193. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1191

Factors Affecting the Efficiency of Rapid Vitrification of in vitroBovine Embryo

Nguyen Thi Ngoc Anh (*) 1 , Nguyen Duc Truong 1 , Nguyen Thi Thu Trang 1 , Nguyen Van Thanh 1 , Do Thi Kim Lanh 1

  • 1 Khoa Thú y, Học viện Nông nghiệp Việt Nam

    • Keywords

    Blastocytes, rapid vitrification, bovine in vitroembryos

    Abstract


    The research was conducted to improve the quality of in introbovine embryo vitrification. Bovine oocyteswere matured thenfertilizedwith frozen-thawed semen at1, 2or 5× 106sperm/ml for 6 hours to find the optimal conditions. Theblastocysts were then rapidly frozen in the medium using TCM199+BSA (Tissue culture medium-199+Bovine serum albumin) or DPBS+FBS (Dulbecco's phosphate-buffered saline+Fetal bovine serum). There was no significant difference in the maturation rateof oocytes matured in BO-IVM or TCM-199 medium. In vitrofertilized bovine oocyteswith frozen-thawed sperm atconcentration of 2 × 106sperm/ml for 6 hours gave the highest fertilization and blastocyst rate. The survival rate after thawing of bovine embryosfrozenin DPBS+FBS and TCM199 + BSA medium was92.96% and 82.71%, respectively, however, there was no significantdifference between the groups. Thehatching rate after thawing of embryos vitrified in TCM 199 + BSA medium reached 54%, significantly higher than that ofembryos vitrifiedin DPBS+FBS medium (28.8%). Inthe gene-edited embryo group, the number of survival embryos after thawing reached 96.03 % compared to 88.82 % in the IVF group. The source of oocytesalso affected the survival rate of embryos after thawing.

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