Isolation, Selection, and Characterisation of Enterococcus faecalisET04Capable of Producing Bacteriocin

Received: 19-09-2022

Accepted: 29-06-2023

DOI:

Views

0

Downloads

0

Issue: Vol. 21 No. 7 (2023)

Section:

KỸ THUẬT VÀ CÔNG NGHỆ

How to Cite:

Hoan, D., Tam, D., Son, D., Truong, N., Dung, P., & Canh, N. (2024). Isolation, Selection, and Characterisation of Enterococcus faecalisET04Capable of Producing Bacteriocin. Vietnam Journal of Agricultural Sciences, 21(7), 868–867. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/1156

Isolation, Selection, and Characterisation of Enterococcus faecalisET04Capable of Producing Bacteriocin

Duong Van Hoan (*) 1 , Dang Thi Thanh Tam 1 , Dinh Truong Son 1 , Nguyen Xuan Truong 1 , Pham Thi Dung 1 , Nguyen Xuan Canh 1

  • 1 Khoa Công nghệ sinh học, Học viện Nông nghiệp Việt Nam

    • Keywords

    Bacteriocin, Enterococcus faecalis, Staphylococcus aureus, Aeromonas jandaei

    Abstract


    The study was carried out to identify Enterococcusstrains capable of producing bacteriocins, orienting the applications as a supplement to aquaculture feeds and replacing the current use of antibiotics. In this study, bacterial strain ET04 was isolated and evaluated for its ability to antagonize against Staphylococcus aureus and Aeromonas jandaei. The research results showed that the bacterial strain ET04 was determined to have the ability to produce bacteriocin that inhibited the growth of the tested microorganisms. The bacteriocin compound produced by strain ET04 was stable at pH 2.0 to 8.0 and most of the antibacterial activity was lost at pH 10; at pH 12.0 the antibacterial activity was completely lost. On MRS medium supplemented with 3% glucose and yeast extract, bacterial strain ET04 showed the best bacteriocin production ability. Combining morphological, physiological, biochemical, and sequence analysis of 16S rRNA genes, strain ET04 was identified as Enterococcusfaecalis ET04.

    References

    Abanoz H.S. & Kunduhoglu B. (2018). Antimicrobial activity of a bacteriocin produced by Enterococcus faecalisKT11 against some pathogens and antibiotic-resistant bacteria. Korean Journal for food science of animal resources.38(5): 1064.

    Ahmadova A., Todorov S.D., Choiset Y., Rabesona H., Zadi T.M., Kuliyev A., De Melo Franco B.D.G., Chobert J.M. & Haertlé T. (2013). Evaluation of antimicrobial activity, probiotic properties, and safety of wild strain Enterococcus faeciumAQ71 isolated from Azerbaijani Motal cheese. Food Control.30(2): 631-641.

    Assefa A. & Abunna F. (2018). Maintenance of fish health in aquaculture: review of epidemiological approaches for prevention and control of infectious disease of fish. Veterinary medicine international.

    Baccouri O., Boukerb A.M., Farhat L.B., Zébré A., Zimmermann K., Domann E., Cambronel M., Barreau M., Maillot O. & Rincé I. (2019). Probiotic potential and safety evaluation of Enterococcus faecalisOB14 and OB15, isolated from traditional tunisian testouri cheese and rigouta, using physiological and genomic analysis. Frontiers in Microbiology.10: 881.

    Cui G., Pan C., Xu P., Li Y., Wang L., Gong B., Li X. & Huang S. (2020). Purification and characterization of a novel bacteriocin produced by Enterococcus faecalisCG-9 from human saliva. Biotechnology & Biotechnological Equipment.34(1): 1224-1233.

    Han Z., Sun J., Lv A., Sung Y., Sun X., Shi H., Hu X., Wang A. & Xing K. (2018). A modified method for genomic DNA extraction from the fish intestinal microflora. AMB Express.8(1): 1-8.

    Hanchi H., Mottawea W., Sebei K. & Hammami R. (2018). The genus Enterococcus: between probiotic potential and safety concerns - an update. Frontiers in Microbiology.9: 1791.

    Hernandez D., Cardell E. & Zarate V. (2005). Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin‐like substance produced by Lactobacillus plantarumTF711. Journal of Applied Microbiology.99(1): 77-84.

    Hwanhlem N., Ivanova T., Biscola V., Choiset Y. & Haertlé T. (2017). Bacteriocin producing Enterococcus faecalisisolated from chicken gastrointestinal tract originating from Phitsanulok, Thailand: Isolation, screening, safety evaluation and probiotic properties. Food Control.78: 187-195.

    Indira M., Venkateswarulu T., Prabhakar K.V., Peele K.A. & Krupanidhi S. (2018). Isolation and characterization of bacteriocin producing Enterococcus casseliflavusand its antagonistic effect on Pseudomonas aeruginosa. Karbala International Journal of Modern Science.4(4): 361-368.

    Liu G., Wang Y., Li X., Hao X., Xu D., Zhou Y., Mehmood A. & Wang C. (2019). Genetic and biochemical evidence that Enterococcus faecalisGr17 produces a novel and sec-dependent bacteriocin, Enterocin Gr17. Frontiers in Microbiology.10: 1806.

    Losteinkit C., Uchiyama K., Ochi S., Takaoka T., Nagahisa K. & Shioya S. (2001). Characterization of bacteriocin N15 produced by Enterococcus faeciumN15 and cloning of the related genes. Journal of Bioscience and Bioengineering.91(4): 390-395.

    Marwa A.S., Hamdi M.A, Ekbal M.I.A., Adham M.A. & Sobhy A.El.S. (2015). Effect of pH, heat treatments and proteinase K enzyme on the activity of Lactobacillus acidophilusbacteriocin. Benha Veterinary Medical Journal. 28 (1): 210-215.

    Nguyễn Văn Thành & Nguyễn Ngọc Trai. (2012). Phân lập và tuyển chọn vi khuẩn Lactobacillussp. Có khả năng ức chế vi khuẩn gây bệnh gan thận mủ và đốm đỏ trên cá tra. Tạp chí Khoa học Trường Đại học Cần Thơ. 23a: 224-234.

    Ogunbanwo S., Sanni A. & Onilude A. (2003). Characterization of bacteriocin produced by Lactobacillus plantarumF1 and Lactobacillus brevisOG1. African Journal of Biotechnology.2(8): 219-227.

    Rajaram G., Manivasagan P., Thilagavathi B. & Saravanakumar A. (2010). Purification and characterization of a bacteriocin produced by Lactobacillus lactisisolated from marine environment. Advance Journal of Food Science and Technology.2(2): 138-144.

    Ramos M., Batista S., Pires M., Silva A., Pereira L., Saavedra M., Ozório R. & Rema P. (2017). Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia. Animal.11(8): 1259-1269.

    Sahoo T.K., Jena P.K., Nagar N., Patel A.K. & Seshadri S. (2015). In vitro evaluation of probiotic properties of lactic acid bacteria from the gut of Labeo rohitaand Catla catla. Probiotics and antimicrobial proteins.7(2): 126-136.

    Subasinghe R. (2017). Regional review on status and trends in aquaculture development in Asia-Pacific-2015. FAO Fisheries and Aquaculture Circular.(C1135/5): I.

    Toit M.D., Franz C., Dicks L. & Holzapfel W. (2000). Preliminary characterization of bacteriocins produced by Enterococcus faeciumand Enterococcus faecalisisolated from pig faeces. Journal of Applied Microbiology.88(3): 482-494.

    Tổng cục Thủy sản (2022). Kết quả sản xuất ngành thủy sản năm 2019. Truy cập từ https://tongcucthuysan.gov.vn/Tin-tức/-Tin-vắn/doc-tin/014196?2020-01-15 = Banner+002 ngày 06/09/2022.

    Vos P., Garrity G., Jones D., Krieg N.R., Ludwig W., Rainey F.A., Karl-Heinz Schleifer & Whitman W. B. (Eds.). (2011). Bergey's manual of systematic bacteriology. Springer Science & Business Media.Vol. 3: 594-611

    Xi Q., Wang J., Du R., Zhao F., Han Y. & Zhou Z. (2018). Purification and characterization of bacteriocin produced by a strain of Enterococcus faecalisTG2. Applied biochemistry and biotechnology.184(4): 1106-1119.