Megalocytivirus liên quan tới bệnh đen thân trên cá rô đồng nuôi thâm canh (Anabas testudineus) ở Việt Nam

Ngày nhận bài: 28-01-2016

Ngày duyệt đăng: 29-03-2016

DOI:

Lượt xem

0

Download

0

Số: Tập 14 Số 4 (2016)

Chuyên mục:

CHĂN NUÔI – THÚ Y – THỦY SẢN

Cách trích dẫn:

Lua, D., May, L., & Hirono, I. (2024). Megalocytivirus liên quan tới bệnh đen thân trên cá rô đồng nuôi thâm canh (Anabas testudineus) ở Việt Nam . Tạp Chí Khoa học Nông nghiệp Việt Nam, 14(4), 620–628. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/273

Megalocytivirus liên quan tới bệnh đen thân trên cá rô đồng nuôi thâm canh (Anabas testudineus) ở Việt Nam

Dang Thi Lua (*) 1, 2, 3, 4 , Le Thi May 5 , Ikuo Hirono 6

  • 1 Viện Nghiên cứu Nuôi trồng Thuỷ sản I
  • 2 Viện nghiên cứu nuôi trồng thủy sản
  • 3 TT Quan trắc môi trường và Bệnh thủy sản miền Bắc, Viện Nghiên cứu nuôi trồng thủy sản 1
  • 4 Center for Environment and Disease Monitoring in Aquaculture, Research Institute for Aquacul
  • 5 Center for Environment and Disease Monitoring in Aquaculture
  • 6 Laboratory of Genome Science, Tokyo University of marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan

    • Từ khóa

    Dark body disease, climbing perch, Anabas testudineus, Megalocytivirus, Iridovirus

    Tóm tắt


    Dark body disease has become a serious problem for climbing perch, Anabas testudineus, cultured in freshwater intensive farming systems in Vietnam. Outbreaks of the disease occur when the fish are about 20-65 days old with 40 - 100% mortality. The main clinical signs of the disease are dark body, haemorrhagic or yellowish liver, and little or no food in the intestines. The disease has recently been attributed to a virus of unknown type. Electron microscopy analysis detected the virus in liver and kidney tissues but not in brain tissues of infected fish. The virion has a symmetric shape with a size of about 150-160nm and is surrounded by a capsid layer. Using primers designed to sequence the major capsid protein (MCP) gene of red seabream iridovirus (RSIV) yielded PCR products from DNA of infected fish. In a phylogenetic analysis based on the partial MCP sequence, the virus clustered with viruses in the genus Megalocytivirus of the family Iridoviridae and it is closely related to infectious spleen and kidney necrosis virus (ISKNV). This study indicated the involvement of a Megalocytivirus in the dark body disease of climbing perch cultured in Vietnam.

    Tài liệu tham khảo

    Bayer-Garner, I. B. (2005). Monkeypox virus: histologic, immunohistochemical and electron microscopic findings. J. Cutan. Pathol.,32: 28-34.

    Biel, S. S., A. Nitsche, A. Kurth, W. Siegert, M. Ozel and H. R. Gelderblom (2004). Detection of human polyomaviruses in urine from bone marrow transplant patients: comparison of electron microscopy with PCR. Clin. Chem., 50: 306-312

    Caipang, C. M., I. Hirono and T. Aoki (2003): Development of a real-time PCR assay for the detection and quantification of red sea bream Iridovirus (RSIV), Fish Pathol., 38: 1-7.

    Chinchar, V. G., S. Essbaues, J. G. He, A. Hyatt, T. Miyazaki, V. Seligy and T. Williams (2005). Iridoviridae. In “Virus Taxonomy: 8th Report of the International Committee on the Taxonomy of Viruses” (Fauquet, C. M., M. A. Mayo, J. Maniloff, U. Desselberger, L. A. Ball, eds). Elsevier, London, pp. 163-175.

    Chua, K. B., E. M. Wong, B. C. Cropp and A. D. Hyatt (2007). Role of electron microscopy in Napah virus outbreak investigation and control. Med. J. Malaysia, 62: 139-142.

    Dang, L. T., H. Kondo, I. Hirono and T. Aoki (2008). Inhibition of red seabream iridovirus (RSIV) replication by small interfering RNA (siRNA) in a cell culture system. Antiviral Res., 77: 142-149

    Dang, L. T., T. V. Phan, T. V. Pham, T. N. T. Ngo (2013). Causative agent of dark body disease in climbing perch (Anabas testudineus) in intensive culture. J. Agri. Rural Dev., (10/2013): 38-42 (ISSN 1859-4581).

    Delhon, G., E. R. Tulman, C. L. Afonso, Z. Lu, J. J. Becnel, B. A. Moser, G. F. Kutish and D. L. Rock (2006). Genome of Invertebrate Iridescent virus type 3 (Mosquito Iridescent virus). J. Virol., 80(17): 8439-8449.

    Do, T. H., Q. T. Bui, H. D. Nguyen and T. M. Nguyen (2004). Aquatic animal pathology. Agriculture Public House, Ho Chi Minh city. (inVietnamese)

    Do, J. W., C. H. Moon, H. J. Kim, M. S. Ko, S. B. Kim, J. H. Son, J. S. Kim, E. J. An, M. K. Kim, S. K. Lee, M. S. Han, S. J. Cha, M. S. Park, M. A. Park, Y. C. Kim, J. W. Kim and J. W. Park (2004). Complete genomic DNA sequence of rock bream iridovirus. Virology, 325: 351-363.

    Doane, F. W. and N. Anderson (1987). Electron microscopy in diagnostic virology: a practical guide and atlas. Cambridge University Press, New York.

    Eaton, H. E., J. Metcalf, E. Penny, V. Tcherepanov, C. Upton and C. R. Brunetti (2007). Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes. Virology Journal 4: 11, doi: 10.1186/1743-422X-4-11.

    Go, J., M. Lancaster, K. Deece, O. Dhungyel and R. Whittington (2006). The molecular epidemiology of iridovirus in Murray cod (Maccullochella peelii peelii) and dwarf gourami (Colisa lalia) from distant biogeographical regions suggests a link between trade in ornamental fish and emerging iridoviral diseases. Mol. Cel. Probes.,20: 212-222.

    Goldsmith, C. S. and S. E. Miller (2009). Modern use of electron microscopy for detection of virues. Clin. Micro. Rev., 22(4): 552 -563.

    Green, M. R. and J. Sambrook (2012) Molecular Cloning: A laboratory manual. Fourth edition. Cold Spring Harbor Laboratogy Press.

    He, J. G., L. Lu, M. Deng, H. H. He, S. P. Weng, X. H. Wang, S. Y. Zhou, Q. X. Long, X. Z. Wang and S. M. Chan (2002). Sequence analysis of the complete genome of an iridovirus isolated from the tiger frog. Virology, 292: 185-197.

    Hyatt, A. D. and P. W. Selleck (1996). Ultrastucture of equine morbillivirus. Virus Res., 43: 1-15.

    Imajoh, M., T. Ikawa and S. Oshima (2007). Characterization of a new fibroblast cell line from a tail fin of red sea bream, Pagrus major, and phylogenetic relationships of a recent RSIV isolate in Japan. Virus Res., 126: 45–52.

    Kurita, J, K. Nakajima, I. Hirono and T. Aoki (2002). Complete genome sequencing of red sea bream Iridovirus (RSIV). Fisheries Sci., 68 (Suppl. II): 1113-1115.

    Le, V. T. (2003). Studies on culture of climping perch (Anabas testudineus) using pellets with different protein concentrations. Master thesis. (In Vietnamese).

    Lu, L., S. Y. Zhou, C. Chen, S. P. Weng, S. M. Chan and J. G. He (2005). Complete genome sequence analysis of an iridovirus isolated from the orange-spotted grouper, Epinephelus coioides. Virology, 339: 81-100.

    Subramaniam, K., M. Shariff, A.R. Omar, M. Hair-Bejo, and B. L. Ong (2014). Detection and molecular characterization of infectious spleen and kidney necrosis virus from major ornamental fish breeding states in Peninsular Malaysia. Journal of Fish Disease, 37: 609-618.

    Tan, W. G., T. J. Barkman, V. G. Chinchar and K. Essani (2004). Comparative genomic analysis of frog virus 3, type species of the genus Ranavirus (family Iridoviridae). Virology, 323: 70-84.

    Tidona, C. A. and G. Darai (1997). The complete DNA sequence of lymphocystis disease virus. Virology, 230: 207-216.

    Thompson JD, Higgins DG, Gibson TJ. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res.,22: 4673-4680.

    Thompson JD, Higgins DG, Gibson TJ. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22: 4673-4680.

    Tsai, C. T. (2005). Complete genome sequnce of the grouper iridovirus and comparison of genomic organization with those of other iridoviruses. J. Virol, 79: 2010-2023.

    Williams, T. (1996). The iridoviruses. Adv. Virus Res. 46: 347-412.

    Williams, T., V. Barbosa-Solomieu and V. G. Chinchar (2005). A decade of advances in iridovirus research. Adv. Virus Res., 65: 173-248.

    Wolf, K. (1988). Fish viruses and Fish Viral Diseases. Cornell University Press. 476pp.

    Wong, C. K., V. L. Young, T. Kleffmann and V. K. Ward (2011). Genomic and proteomic analysis of Invertebrate iridovirus type 9. J. Virol., 85(15): 7900-7911.

    Yanong, R. P. E. and T. B. Waltzek (2010). Megalocytivirus infections in fish, with emphasis on ornamental species. University of Florida, Institute of food and Agricultural sciences. Extension FA, 182: 1-7. http://edis.ifas.ufl.edu/fa182.

    Zhang, Q. Y., F. Xiao, J. Xie, Z. Q. Li and J. F. Gui(2004). Complete genome sequence of lymphocystis disease virus isolated from China. J. Vivol., 78: 6982-6994.