PROTEIN AND LIPID RECOVERY FROM TUNA HEAD USING INDUSTRIAL PROTEASE

Ngày nhận bài: 28-09-2013

Ngày duyệt đăng: 22-12-2013

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Số: Tập 11 Số 8 (2013)

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KỸ THUẬT VÀ CÔNG NGHỆ

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Huong, N. (2024). PROTEIN AND LIPID RECOVERY FROM TUNA HEAD USING INDUSTRIAL PROTEASE. Tạp Chí Khoa học Nông nghiệp Việt Nam, 11(8), 1150–1158. http://testtapchi.vnua.edu.vn/index.php/vjasvn/article/view/77

PROTEIN AND LIPID RECOVERY FROM TUNA HEAD USING INDUSTRIAL PROTEASE

Nguyen Thi My Huong (*) 1

  • 1 Faculty of Food Technology - Nha Trang University

    • Từ khóa

    Enzymatic hydrolysis, protein hydrolysate, protein and lipid recovery, tuna head

    Tóm tắt


    Protein and lipid recovery from yellowfin tuna heads by enzymatic hydrolysis was studied. Hydrolysis of tuna head was carried out using 0.5% Protamex at 45°C without pH control for 120 minutes with a water/material ratio of 1:1 (ml/g). Nitrogen recovery, amino acid composition of protein hydrolysate, lipid recovery and fatty acid composition of fish oil obtained from hydrolysis of tuna heads were determined. Results showed that after 120 minutes of hydrolysis, the nitrogen and lipid recoveries were 70.3% and 65.4% respectively. Protein hydrolysate from yellowfin tuna heads had of 80% protein, 1.3% lipid and 7.9% ash. Protein hydrolysate had high content of essential amino acids (33.67%). The fish oil obtained from hydrolysis of tuna heads was rich in omega-3 fatty acids (18.99%), especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). The content of omega-6 fatty acids was 4.37%. Fatty acids with high contents in tuna head oil were palmitic acid (29.75%), oleic acid (16.76%) and docosahexaenoic acid (14.56%).

    Tài liệu tham khảo

    Aspmo,S.I., Horn,S.J., Eijsink,V.G.H. (2005).Enzymatic hydrolysis of Atlantic cod (Gadus morhua L.) viscera. Process Biochemistry, 40: 1957-1966.

    Batista, I., Ramos, C., Mendonca, R., Nunes, M. L., (2009). Enzymatic hydrolysis of sardine (Sardina pilchardus) by-products and lipid recovery. Journal of Aquatic Food Product Technology, 18: 120-134.

    Benjakul, S., Morrissey, M. T., (1997). Protein hydrolysates from Pacific whiting solid waste. J Agric. Food Chemistry, 45:3423-30.

    Berge, G.M., Storebakken, T.,(1996). Fish protein hydrolysate in starter diets for Atlantic salmon (Salmo salar) fry. Aquaculture, 145(1-4):205-212.

    Chalamaiah, M., Dinesh kumar , B., Hemalatha, R., Jyothirmayi, T., (2012). Fish protein hydrolysates: Proximate composition, amino acid composition, antioxidant activities and applications: A review. Food Chemistry, 135 (4): 3020 -3038.

    Dauksas, E., Falch, E., Slizyté, R., Rustad, T., (2005). Composition of fatty acids and lipid classes in bulk products generated during enzymic hydrolysis of cod (Gadus morhua) by-products. Process Biochemistry, 40: 2659-2670.

    Dumay, J., Donnay-Moreno, C., Barnathan, G., Jaouen, P., Bergé, J. P., (2006). Improvement of lipid and phospholipid recoveries from sardine (Sardina pilchardus) viscera using industrial proteases, Process Biochemistry, 41: 2327-2332.

    FAO/WHO,(1990). Energy and protein requirements. Report of joint FAO/WHO/UNU Expert Consultation Technical Report. Geneva, FAO/WHO and United Nations University

    Folch,J., Lees,N., Sloan-Stanley,G.H.,(1957). A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226: 497- 509.

    Guérard, F., Guimas, L., Binet, A., (2002). Production of tuna waste hydrolysates by a commercial neutral protease preparation. J Mol Catal B-Enzym., 19-20: 489-98.

    Kechaou, E.,S., Dumay, J., Donnay-Moreno, C., Jaouen, P., Gouggou, J.P., Bergé, J. P., Amar, R.,B. (2009). Enzymatic hydrolysis of cuttlefish (Sepia officialis) and sardine (Sardina pichardus) viscera using commercial proteases: Effects on lipid distribution and amino acid composition. J Biosci Bioeng., 107(2): 158-64.

    Liaset, B., Nortvedt, R., Lied, E., Espe, M., (2002). Studies on the nitrogen recovery in enzymatic hydrolysis of Atlantic salmon (Salmo salar, L.) frames by Protamex™protease. Process Biochemistry, 37: 1263-1269.

    Mbatia, B., Adlercreutz, D., Adlercreutz, P., Mahadhy, A., (2010). Enzymatic oil extraction and positional analysis of -3 fatty acids in Nile perch and salmon heads. Process Biochemistry, 45: 815-819.

    Nguyen, H. T. M., Sylla, K. S. B., Randriamahatody, Z., Donnay-Moreno, C., Moreau, J., Tran, T. L., Bergé, J. P., (2011). Enzymatic hydrolysis of yellowfin tuna (Thunnus albacares) by-products using Protamex protease. Food Technology and Biotechnology, 49 (1): 48-55.

    Nguyen, H. T. M., Pérez-Gálvez, R., Bergé, J. P., (2012). Effect of diets containing tuna head hydrolysates on the survival and growth of shrimp Penaeus vannamei. Aquaculture. 324-325:127-134.

    Noriega-Rodríguez, J.A., Ortega-García, J., Angulo-Guerrero, O; García, H. S., Medina-Juárez, L. A., Gámez-Mezac, N., (2009). Oil production from sardine (Sardinops sagax caerulea). CyTA - Journal of Food. Vol. 7 (3): 173-179.

    Refstie,S., Olli,J.J., Standal,H., (2004). Feed intake, growth and protein utilization by post-smolt Alantic salmon (Salmo salar) in response to graded levels of fish protein hydrolysate in the diet. Aquaculture, 239: 331-349

    Sathivel, S., Bechtel, P.J., Babbitt, J., Smiley, S., Crapo, C., Reppond, K.D., Prinyawiwatkul,W.,(2003). Biochemical and functional properties of herring (Clupea harengus) byproduct hydrolysates. Food Science, 68: 2196-2200.

    Sathivel, S., Smiley, S., Prinyawiwatkul, W., Bechtel, P. J. (2005). Functional and nutritional properties of red salmon (Oncorhynchus nerka) enzymatic hydrolysates. Food Science, 70(6): 401-406.

    Shahidi, F., Han, X.,Q, Synowieck, J., 1995. Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chem, 53: 285-93.

    Shimada, Y., Maruyama, K., Sugihara, A., Moriyama, S., Tominaga, Y., (1997). Purification of docosahexaenoic acid from tuna oil by a two-step enzymatic method: hydrolysis and selective esterification. Journal of the American Oil Chemists’ Society.74:1441-1446.

    Šližyte, R., Dauksas, E., Falch, E., Storro, I., Rustad, T., 2005. Yield and composition of different fractions obtained after enzymatic hydrolysis of cod (Gadus morhua) by-products. Process Biochem., 40: 1415-1424.

    Stansby, M.E., Schlenk, H., Gruger, E.H., (1990). Fatty acid composition of fish. In Stansby, M.E, Fish oil in nutrition. 6-39. New York: Van Nostrand Reinhold.

    Tang, H.G., Wu, T.X., Zhao, Z.Y., Pan, X.D., (2008). Effects of fish protein hydrolysate on growth performance and humoral immune response in large yellow croaker (Pseudosciaena crocea R.). Zhejiang Univ Sci B., 9: 684-690.

    Yu, S.Y, Tan, L., (1990). Acceptability of crackers (‘keropok’) with fish protein hydrolysate. International J Food Sci. Tech., 25: 204-8.