Received: 29-03-2023 / Accepted: 29-06-2023
This study was carried out to determine the polymorphism of growth hormone (GH), growth hormone receptor (GHR) genes and the association of the polymorphisms with body weight in Lien Minh chicken. Total 835 birds (329 males, 506 females) with body weight form 1 day old to 20 weeks of age were observed. Genomic DNA was extracted form blood and analyzed for single nucleotide polymorphisms (SNPs) by PCR-RFLP. For G662A/GH, three genotypes, AA, AG and GG were found with the frequency of allele A was 0.91 and allele G was 0.09; for G1705A/GH, also 3 genotypes: AA, AG and GG with allele A frequency of 0.18 and allele G of 0.82 were identified; for C571T/GHR polymorphism, 3 genotypes: CC, CT and TT with frequency of allele C was 0.74 and allele T was 0.26. Chickens with the GG genotype of G662A/GH polymorphism had a significantly higher body weight than the individuals with the AA genotypes from 11 to 20 weeks of age (P <0.05). For the G1705A/GH polymorphism, chickens with AA genotype had a significantly higher weight than chickens with AG genotype from 12 to 17 weeks of age (P <0.05), and significantly higher than chickens with GG genotype from 8 to 20 weeks of age (P <0.05). No association between C571T/GHR polymorphism with body weight trait in Lien Minh chickens (P >0.05) was detected in this study.