Received: 28-09-2020 / Accepted: 16-12-2020
ThemajoraimofthisworkwastoproducepolyclonalantibodiesfordiagnosisofPassifloramottlevirus(PaMoV),aputativenovelpotyvirus(thegenusPotyvirus,thefamilyPotyviridae),whichwasfirstdiscoveredfrompassionfruit(Passifloraedulis)andnamedinVietnam.Theentiregeneencodingcoatprotein(CP)ofPaMoVwasclonedonpET28avectorandtheexpressionofthisproteinwasevaluatedintheEscherichiacoli(E.coli)strainRosetta(DE3).ThedenaturedrecombinantPaMoVCPproteinwasusedasan antigenforrabbitinjection.ThetiterandspecificityoftherabbitantiserumwereevaluatedbyDot-ImmunobindingAssay(DIBA)andPlateTrappedAntigenEnzyme-LinkedImmunosorbentAssay(PTA-ELISA).Theexperimental resultsshowed: (i) Rosetta(DE3)wasasuitablehoststrainforexpressionofthePaMoVCPprotein, (ii) thePaMoV CP antiserumdidnotreactwithhealthypassionfruitsapanddetected easily cognate inpassionfruitleaves, and (iii) the antiserumalsoreactedwithtwopotyviruses,Telosmamosaicvirus(TelMV)andEastAsianpassifloravirusstrainIB(EAPV-IB),bothareinfectingpassionfruitinVietnam.